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Immunohistochemical characteristics of regenerated bone after surgical therapy of advanced ligature-induced peri-implantitis defects

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Abstract

Objectives

This study seeks to assess and compare immunohistochemical characteristics of regenerated and pristine bone areas following surgical therapy of advanced peri-implantitis.

Methods

At ligature-induced peri-implantitis defects, the intrabony component was filled with a natural bone mineral (NBM), and the supracrestal component was treated by either an equine bone block (EB) or implantoplasty. NBM and EB were soak-loaded with rhBMP-2 or sterile saline. Membrane (i.e., native collagen) protected sites were submerged for 12 weeks. Osteocalcin (OC) and transglutaminase 2 (TG2; angiogenesis) antigen reactivity was assessed within the augmented- (AA) and pristine bone (PB) areas at non-exposed sites (n = 39 defects).

Results

In all groups investigated, mean OC (AA, 0.5 ± 0.4 to 1.9 ± 2.9 %/PB, 1.7 ± 2.6 to 3.5 ± 6.5 %) and TG2 (AA, 0.6 ± 0.5 to 1.3 ± 1.5 %/PB, 0.5 ± 0.5 to 1.6 ± 1.9 %) values within AA did not significantly differ from those values assessed within PB (P > 0.05, respectively).

Conclusions

AA formed in different treatment groups may not be considered as qualitatively (i.e., OC and TG2) compromised bone.

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Acknowledgments

We kindly appreciate the skills and commitment of Mrs Hartig, Mr Kaiser, and Mr Lommen (Department of Oral Surgery, Heinrich Heine University, Düsseldorf, Germany) in the preparation of the histological specimens.

Source of funding

The study was funded by the Osteology Foundation, Lucerne, Switzerland and the Camlog Foundation, Basel, Switzerland. The study materials and devices were kindly provided by Camlog Biotechnologies AG, Basel, Switzerland. Geistlich Biomaterials, Wolhusen, Switzerland.

Conflict of interest statement

The authors declare that they have no conflict of interests related to this study.

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Authors and Affiliations

  1. Department of Oral Surgery, Westdeutsche Kieferklinik, Heinrich Heine University, 40225, Düsseldorf, Germany

    Frank Schwarz, Ilja Mihatovic, Vladimir Golubovic & Jürgen Becker

  2. Animal Research Institute, Heinrich Heine University, Düsseldorf, Germany

    Martin Sager

Authors
  1. Frank Schwarz
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  2. Ilja Mihatovic
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  3. Vladimir Golubovic
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  4. Jürgen Becker
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  5. Martin Sager
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Corresponding author

Correspondence to Frank Schwarz.

Additional information

Frank Schwarz and Ilja Mihatovic equally contributed to the study and are considered as joint first authors.

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Schwarz, F., Mihatovic, I., Golubovic, V. et al. Immunohistochemical characteristics of regenerated bone after surgical therapy of advanced ligature-induced peri-implantitis defects. Clin Oral Invest 18, 1679–1686 (2014). https://doi.org/10.1007/s00784-013-1138-5

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  • DOI: https://doi.org/10.1007/s00784-013-1138-5

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