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Expression of growth factors during the healing process of alveolar ridge augmentation procedures using autogenous bone grafts in combination with GTR and an anorganic bovine bone substitute

An immunohistochemical study in the sheep

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Abstract

Objectives

This study aims to evaluate the expression of various immunohistochemical growth factors and vascularization markers in augmentation on the mandible comparing onlay bone grafts and Guided Bone Regeneration (GBR).

Materials and methods

Using a sheep in vivo model, autogenous bone grafts were harvested from the iliac crest. A combination of a resorbable collagen membrane (CM) and a Deproteinized Bovine Bone Material (DBBM) was performed. This modification of the host side was compared with an onlay bone graft control group. Expression of different vascularization markers was compared between these groups.

Results

The expression of revascularization markers was significantly higher within the modification of the host side using GBR and DBBM. Regarding different graft regions, a significantly higher expression within the bone graft using GBR and DBBM could be observed in staining on bone morphogenetic protein-2 (BMP-2) (5.75 vs. 3.55), vascular endothelial growth factor (VEGF) (3.08 vs. 1.64), VEGF Receptor 1 (VEGFR-1) and VEGF Receptor 2 (VEGFR-2) (4.88 vs. 2.24 and 5.06 vs. 2.74), and endothelial nitric oxide synthase (eNOS) and inducible nitric oxide synthase (iNOS) (5.29 vs. 3.28 and 5.22 vs. 3.09; p = 0.000, all others p < 0.05), whereas the control group showed a higher rate of resorption during the surveillance period until euthanasia of sheep after 16 weeks.

Conclusion

The use of GBR and DBBM in the transplantation process of autogenous bone grafts compared with the therapeutical use of certain growth factors may enhance vascularization and lower atrophy and resorption.

Clinical relevance

The use of a combination of GBR and DBBM in augmentation procedures on the mandible shows less resorption than simple onlay bone grafts and seems to be superior in a clinical use.

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Acknowledgments

This work was supported by a grant of Deutsche Forschungsgemeinschaft, Germany.

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Authors and Affiliations

  1. Department of Oral and Maxillofacial Plastic Surgery, University of Wuerzburg, Pleicherwall 2, D-97070, Wuerzburg, Germany

    Steffen Koerdt, Andreas Wannhoff, Alexander C. Kübler & Tobias Reuther

  2. Medicine & Aesthetics, Clinic for Oral, Maxillofacial and Plastical Surgery, Lenbachplatz 2a, D-80333, Munich, Germany

    Oliver Ristow

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  1. Steffen Koerdt
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Correspondence to Steffen Koerdt.

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Koerdt, S., Ristow, O., Wannhoff, A. et al. Expression of growth factors during the healing process of alveolar ridge augmentation procedures using autogenous bone grafts in combination with GTR and an anorganic bovine bone substitute. Clin Oral Invest 18, 179–188 (2014). https://doi.org/10.1007/s00784-013-0938-y

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  • DOI: https://doi.org/10.1007/s00784-013-0938-y

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