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Proliferation and osteogenic differentiation of osteoblast-like cells obtained from two techniques for harvesting intraoral bone grafts

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Abstract

Objectives

The aims of our study were to verify the presence of viable osteoblasts in samples of bone tissue obtained by drilling or from cortico-cancellous bone blocks and to assess their growth and differentiation capacities.

Materials and methods

Bone tissue samples were processed independently and cultured in Dulbecco’s modified Eagle medium, in a CO2 incubator at 37 °C. The proliferative capacity of osteoblasts was determined by spectrophotometry (MTT) at 24 and 48 h of culture. Cell cycle was analysed by flow cytometry. Cell differentiation was studied by red alizarin staining of nodules formed in mineralisation medium and by analysis of alkaline phosphatase activity.

Results

In comparison to bone block-derived osteoblasts, the proliferative capacity was greater at 24 and 48 h of culture (P < 0.001) in the drilling-derived osteoblasts, which showed significantly increased G2/M (P = 0.014) and S (P < 0.001) phases in the cell cycle study. The number of mineralised nodules was proportional to the incubation time, with no differences between the two types of sample, which also did not significantly differ in alkaline phosphatase activity.

Conclusion

Superior autograft material is obtained by harvesting particulate bone from low-speed drilling fragments than from a cortico-cancellous bone block.

Clinical relevance

These results suggest that bone obtained from low-speed drilling is a simple and effective alternative to the classic procedure for obtaining bone tissue.

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Acknowledgements

This study was supported by research group BIO277 (Junta de Andalucía) and by the Department of Nursing of the Health Sciences School of the University of Granada.

Conflicts of interest statement

The authors declare that they have no conflict of interest.

Author information

Authors and Affiliations

  1. Department of Stomatology, School of Dentistry, University of Granada, Colegio Máximo s/n, Campus Universitario de Cartuja, 18071, Granada, Spain

    Francisco Javier Manzano-Moreno, Juan Bautista Rodríguez-Martínez, Manuel Francisco Vallecillo-Capilla & Candela Reyes-Botella

  2. Biomedical Group (BIO277), Department of Nursing, Faculty of Health Sciences, University of Granada, Granada, Spain

    Francisco Javier Manzano-Moreno, Javier Ramos-Torrecillas, Concepción Ruiz, Olga García-Martínez & Candela Reyes-Botella

  3. Institute of Neuroscience, Parque Tecnológico Ciencias de la Salud, Armilla (Granada), University of Granada, Granada, Spain

    Concepción Ruiz

Authors
  1. Francisco Javier Manzano-Moreno
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  2. Juan Bautista Rodríguez-Martínez
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  3. Javier Ramos-Torrecillas
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  4. Manuel Francisco Vallecillo-Capilla
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  6. Olga García-Martínez
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  7. Candela Reyes-Botella
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Correspondence to Francisco Javier Manzano-Moreno.

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Manzano-Moreno, F.J., Rodríguez-Martínez, J.B., Ramos-Torrecillas, J. et al. Proliferation and osteogenic differentiation of osteoblast-like cells obtained from two techniques for harvesting intraoral bone grafts. Clin Oral Invest 17, 1349–1356 (2013). https://doi.org/10.1007/s00784-012-0811-4

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  • DOI: https://doi.org/10.1007/s00784-012-0811-4

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