Clinical Oral Investigations

, Volume 17, Issue 3, pp 805–814 | Cite as

Influence of a mouthwash containing hydroxyapatite microclusters on bacterial adherence in situ

  • C. HannigEmail author
  • S. Basche
  • T. Burghardt
  • A. Al-Ahmad
  • M. Hannig
Original Article



The aim of the present study was to investigate the efficacy of a new preparation in dental prophylaxis containing zinc-carbonate hydroxyapatite microclusters (Biorepair) for oral biofilm management.

Methods and materials

Initial biofilm formation was carried out in situ with bovine enamel slabs fixed to individual upper jaw splints worn by six subjects. Rinses with the customary preparation as well as with subfractions (hydroxyapatite microclusters in saline solution; liquid phase without particles) were adopted for 1 min in situ after 1 min of pellicle formation, and the bacterial colonization was recorded after 6 h and 12 h, respectively. Rinses with chlorhexidine served as a reference. The adherent microorganisms were quantified and visualized using DAPI staining and live–dead staining (BacLight). Furthermore, the effects on Streptococcus mutans bacteria were tested in vitro (BacLight).


Application of the customary preparation and of the separate components distinctly reduced the initial bacterial colonization of the enamel surface in situ as visualized and quantified with all techniques. After 12 h, 1.3 × 107 ± 2.0 × 107 bacteria/cm² were detected on unrinsed control samples with DAPI staining; 2.4 × 106 ± 3.3 × 106 after application of Biorepair (12 h after CHX-rinse; 1.3 × 105 ± 9.2 × 104). Also, pure hydroxyapatite microclusters in saline solution (2.1 × 106 ± 3.0 × 106) as well as the liquid phase without particles (5.1 × 105 ± 3.3 × 105) reduced the amount of adherent bacteria. Furthermore, antimicrobial effects on S. mutans were observed in vitro.


The preparation is an effective compound for biofilm management in the oral cavity due to antiadherent and antibacterial effects.

Clinical relevance

The tested mouthrinse seems to be a reasonable amendment for dental prophylaxis.


Nanomaterial Mouthwash Hydroxyapatite Biofilm Pellicle Bacterial adherence 



We would like to thank Bettina Spitzmüller, University of Freiburg, for the excellent support in the laboratory.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer-Verlag 2012

Authors and Affiliations

  • C. Hannig
    • 1
    Email author
  • S. Basche
    • 1
  • T. Burghardt
    • 1
  • A. Al-Ahmad
    • 2
  • M. Hannig
    • 3
  1. 1.Clinic of Operative Dentistry, Faculty of Medicine ‘Carl Gustav Carus’Technical University of DresdenDresdenGermany
  2. 2.Department of Operative Dentistry and PeriodontologyUniversity of FreiburgFreiburgGermany
  3. 3.Clinic of Operative Dentistry, Periodontology and Preventive Dentistry, University HospitalSaarland UniversityHomburgGermany

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