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Clinical and microbiological effects of ozone nano-bubble water irrigation as an adjunct to mechanical subgingival debridement in periodontitis patients in a randomized controlled trial

Clinical Oral Investigations volume 17, pages 379–388 (2013)Cite this article

Abstract

Aim

Ozone nano-bubble water (NBW3) seems to be suitable as an adjunct to periodontal treatment owing to its potent antimicrobial effects, high level of safety, and long storage stability. The aim of the present study was to evaluate the clinical and microbiological effects of NBW3 irrigation as an adjunct to subgingival debridement for periodontal treatment.

Methods

Twenty-two subjects were randomly assigned to one of the two treatment groups: full-mouth mechanical debridement with tap water (WATER) or full-mouth mechanical debridement with NBW3 (NBW3). Clinical examination was performed at baseline and 4 and 8 weeks after treatment. Microbiological examination was carried out just before and after treatment and at 1 and 8 weeks posttreatment.

Results

There were significant improvements in all clinical parameters after 4 weeks in both groups. The reduction in the probing pocket depth and the clinical attachment gain after 4 and 8 weeks in the NBW3 group were significantly greater than those in the WATER group. Moreover, only the NBW3 group showed statistically significant reductions in the mean total number of bacteria in subgingival plaque over the study period.

Conclusions

The present study suggests that subgingival irrigation with NBW3 may be a valuable adjunct to periodontal treatment.

Clinical relevance

This study verified the potential of new antimicrobial agent, MNW3, as an adjunct to periodontal treatment.

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Acknowledgments

This work was supported by a grant-in-aid for scientific research (B) #18390561 to Y.I. and a grant-in-aid for scientific research (C) (2) #21592620 to S.A. from the Ministry of Education, Culture, Sports, Science, and Technology.

Conflict of interest

The authors declare that they have no conflict of interest.

Author information

Affiliations

  1. Section of Periodontology, Department of Hard Tissue Engineering, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan

    Sae Hayakumo, Shinichi Arakawa & Yuichi Izumi

  2. Hyperbaric Medical Center, Hospital of Medicine, Tokyo Medical and Dental University, Tokyo, Japan

    Yoshihiro Mano

  3. Global Center of Excellence (GCOE) Program, Tokyo Medical and Dental University, Tokyo, Japan

    Yuichi Izumi

Authors
  1. Sae Hayakumo
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  2. Shinichi Arakawa
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  3. Yoshihiro Mano
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  4. Yuichi Izumi
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Correspondence to Shinichi Arakawa.

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Hayakumo, S., Arakawa, S., Mano, Y. et al. Clinical and microbiological effects of ozone nano-bubble water irrigation as an adjunct to mechanical subgingival debridement in periodontitis patients in a randomized controlled trial. Clin Oral Invest 17, 379–388 (2013). https://doi.org/10.1007/s00784-012-0711-7

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  • DOI: https://doi.org/10.1007/s00784-012-0711-7

Keywords

  • Adjunctive antimicrobial agents
  • Irrigation
  • Ozone nano-bubble water
  • Periodontitis
  • Subgingival debridement