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Inhibition of the activity of matrix metalloproteinase 2 by triethylene glycol dimethacrylate

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Abstract

The aim of this study was to evaluate the effect of different concentrations of triethylene glycol dimethacrylate (TEGDMA) on the inhibition of matrix metalloproteinase 2 (MMP-2). Mouse gingival explants were cultured overnight in DMEM and the expression of secreted enzymes was analyzed by gelatin zymography in buffers containing 5 mM CaCl2 (Tris-CaCl2) in 50 mM Tris-HCl buffer with the addition of TEGDMA at different concentrations (0.62%, 1.25%, 2.5%, or 5.0% (v/v)). The gelatinolytic proteinase present in the conditioned media was characterized as matrix metalloproteinase by means of specific chemical inhibition. The matrix metalloproteinases present in the conditioned media were characterized as MMP-2 by immunoprecipitation. The eletrophoretic bands were scanned and the transmittance values were analyzed. Data was plotted and submitted to linear regression to investigate MMP-2 inhibition as a function of TEGDMA concentration. Three major bands were detected in the zymographic assays. These bands were characterized as MMP-2. Zymogene (72 kDa), intermediate (66 kDa) and active forms of MMP-2 (62 kDa) were inhibited by TEGDMA in a dose-dependent way. These findings suggest that TEGDMA could inhibit MMP-2 expression even at small concentrations.

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Acknowledgements

The authors are grateful to CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior) for the scholarship, to Esstech Inc., for the donation of the reagents, and to Angelus Science and Technology for partial financial support for the research project.

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The authors declare that they have no conflict of interest.

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Correspondence to Rodrigo Varella de Carvalho.

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de Carvalho, R.V., Ogliari, F.A., Marques, M.R. et al. Inhibition of the activity of matrix metalloproteinase 2 by triethylene glycol dimethacrylate. Clin Oral Invest 15, 643–648 (2011). https://doi.org/10.1007/s00784-010-0443-5

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  • DOI: https://doi.org/10.1007/s00784-010-0443-5

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