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Cellular death but not genetic damage in oral mucosa cells after exposure to digital lateral radiography

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Abstract

The aim of the present study was to evaluate DNA damage (micronucleus) and cellular death (pyknosis, karyolysis, and karyorrhexis) in exfoliated buccal mucosa cells from individuals following digital lateral radiography. A total of 30 healthy patients (15 men and 15 women) indicated to the orthodontic therapy were submitted to digital lateral X-ray. Exfoliated oral mucosa cells were collected immediately before the X-ray exposure and after 10 days. The results pointed out no significant statistically differences (p > 0.05) of micronucleated oral mucosa cells. On the other hand, X-ray was able to increase other nuclear alterations closely related to cytotoxicity such as karyorrhexis, pyknosis, and karyolysis. In summary, these data indicate that exposure to digital lateral radiography may not be a factor that induced chromosomal damage, but it is able to promote cytotoxicity.

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Acknowledgments

This work was supported by grants from Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, grant number: 07/01228-4). Daniel A. Ribeiro is a recipient of the CNPq fellowship.

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Authors and Affiliations

  1. Departamento de Patologia, Universidade Federal de Sao Paulo, São Paulo, Brazil

    Daniel A. Ribeiro & Sandra R. Miranda

  2. Departamento de Biociências, Universidade Federal de São Paulo—UNIFESP, Av. Ana Costa 95, 11060-001, Santos, São Paulo, Brazil

    Daniel A. Ribeiro & Renan Pozzi

  3. Department of Dentomaxillofacial Radiology, São Paulo Methodist University, UMESP, Sao Bernardo do Campo, São Paulo, Brazil

    Eduardo K. Sannomiya

  4. Department of Orthodontics, São Paulo Methodist University, UMESP, Sao Bernardo do Campo, São Paulo, Brazil

    Eduardo K. Sannomiya & Fernanda Angelieri

Authors
  1. Daniel A. Ribeiro
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  2. Eduardo K. Sannomiya
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  3. Renan Pozzi
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  4. Sandra R. Miranda
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  5. Fernanda Angelieri
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Correspondence to Daniel A. Ribeiro.

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Ribeiro, D.A., Sannomiya, E.K., Pozzi, R. et al. Cellular death but not genetic damage in oral mucosa cells after exposure to digital lateral radiography. Clin Oral Invest 15, 357–360 (2011). https://doi.org/10.1007/s00784-010-0402-1

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  • DOI: https://doi.org/10.1007/s00784-010-0402-1

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