Abstract
This review summarizes the in vivo experiments carried out by our group after implantation of bioactive molecules (matricellular molecules) into the exposed pulp of the first maxillary molar of the rat or the mandibular incisor of rats and mice. We describe the cascade of recruitment, proliferation and terminal differentiation of cells involved in the formation of reparative dentin. Cloned immortalized odontoblast progenitors were also implanted in the incisors and in vitro studies aimed at revealing the signaling pathways leading from undifferentiated progenitors to fully differentiated polarized cells. Together, these experimental approaches pave the way for controlled dentin regenerative processes and repair.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Decup F, Six N, Palmier B, Buch D, Lasfargues J-J, Salih E, Goldberg M (2000) Bone sialoprotein-induced reparative dentinogenesis in the pulp of rat’s molar. Clin Oral Investig 4:110–119
Deutch D, Haze-Filderman A, Blumenfeld A, Dafni L, Leiser Y, Shay B, Gruenbaum-Cohen Y, Rosenfeld E, Fermon E, Zimmermann B, Haegewald S, Bernimoulin J-P, Taylor AL (2006) Amelogenin, a major structural protein in mineralizing enamel, is also expressed in soft tissues: brain and cells of the hematopoietic system. Eur J Oral Sci 114(Suppl 1):189–189
Goldberg M, Six N, Decup F, Buch D, Soheili Majd E, Lasfargues J-J, Salih E, Stanislawski L (2001) Application of bioactive molecules in pulp capping situations. Adv Dent Research 15:91–95
Goldberg M, Six N, Decup F, Lasfargues J-J, Salih E, Tompkins K, Veis A (2003) Bioactive molecules and the future of pulp therapy. Am J Dent 16:66–76
Goldberg M, Smith A (2004) Cells and extracellular matrices of dentin and pulp. A biological basis for repair and tissue engineering. Crit Rev Oral Biol Med 15:13–27
Goldberg M, Lacerda-Pinheiro S, Jegat N, Six N, Septier D, Priam F, Bonnefoix M, Tompkins K, Chardin H, Denbesten P, Veis A, Poliard A (2006) The impact of bioactive molecules to stimulate tooth repair and regeneration as part of restorative dentistry. Dent Clin North Am—(Special Issue on Tissue Engineering) 50:277–298
Nakashima M (1990) The induction of reparative dentine in the amputated dental pulp of the dog by bone morphogenetic protein. Arch Oral Biol 35:493–497
Nakashima M (1994) Induction of dentine in amputated pulp of dogs by recombinant human bone morphogenetic proteins-2 and -4 with collagen matrix. Arch Oral Biol 39:1085–1089
Priam F, Ronco V, Locker M, Bourd K, Bonnefoix M, Duchêne T, Bitard J, Wurtz T, Kellermann O, Goldberg M, Poliard A (2005) New cellular models for tracking the odontoblast phenotype. Arch Oral Biol 50:271–277
Priam F, Lacerda S, Bonnefoix M, Veis A, Goldberg M, Poliard A (2008) The phenotype of immortalized odontoblast progenitors. Adv Dent Res in press
Rutherford B, Fitzgerald M (1995) A new biological approach to vital pulp therapy. Crit Rev Oral Biol Med 6:218–229
Rutherford RB, Wahle J, Tucker M, Rueger D, Charette M (1993) Induction of reparative dentine formation in monkey by recombinant human osteogenic protein-1. Arch Oral Biol 38:571–576
Rutherford RB, Spängberg L, Tucker M, Rueger D, Charette M (1994) The time-course of the induction of reparative dentine formation in monkeys by recombinant human osteogenic protein-1. Arch Oral Biol 39:833–838
Six N, Lasfargues J-J, Goldberg M (2000) In vivo study of the pulp reaction to Fuji IX, a glass ionomer cement. J Dent 28:413–422
Six N, Decup F, Lasfargues J-J, Salih E, Goldberg M (2002) Osteogenic proteins (bone sialoprotéin and bone morphogenetic protein-7) and dental pulp mineralization. J Material Sciences-Material in Medicine 13:225–232
Six N, Lasfargues J-J, Goldberg M (2002) Recombinant human bone morphogenetic protein-7 (osteogenic protein-1) induces differential repair responses in the coronal and radicular areas of the exposed rat molar pulp. Arch Oral Biol 47:177–187
Six N, Tompkins K, Lasfargues J-J, Veis A, Goldberg M (2002) Bioengineered reparative dentin and pulp mineralization. Proceedings Dentin–Pulp Complex. Chiba 2001, Tokyo, Quintessence pp 52–59
Six N, Tompkins K, Septier D, Veis A, Goldberg M (2004) Recruitment and characterization of the cells involved in reparative dentin formation in the exposed rat molar pulp after implantation of amelogenin gene splice products A+4 and A−4. Oral Biosciences & Medicine 1:35–44
Six N, Septier D, Chaussain-Miller C, Blacher R, DenBesten P, Goldberg M (2007) Dentonin, a MEPE fragment, initiates pulp healing response to injury. J Dent Res 86:780–785
Veis A, Tompkins K, Alvares K, Wei K, Wang L, Wang XS (2000) Specific amelogenin gene splice products have signaling effects on cells in culture and in implants in vivo. J Biol Chem 275:41263–41272
Veis A, Tompkins K, Goldberg M (2002) Amelogenin peptides have unique milieu-dependent roles in morphogenic path determination. In: Hascall VC, Kuettner KE (eds) The many faces of osteoarthritis. Birkhäuser, Basel, Switzerland, pp 101–105
Acknowledgements
These investigations were carried out with the support of a CNRS/INSERM grant (Ingénierie tissulaire 2000), INSERM grant Cellules souches adultes 2003 and Fondation de l'Avenir ETS-407). This article is dedicated to more than 25 years of friendship between the first author and Prof. Dr. Gottfried Schmalz.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Goldberg, M., Lacerda-Pinheiro, S., Priam, F. et al. Matricellular molecules and odontoblast progenitors as tools for dentin repair and regeneration. Clin Oral Invest 12, 109–112 (2008). https://doi.org/10.1007/s00784-007-0172-6
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00784-007-0172-6