Clinical Oral Investigations

, Volume 11, Issue 2, pp 143–147 | Cite as

The effect of bee propolis on recurrent aphthous stomatitis: a pilot study

  • Nachum SametEmail author
  • Caroline Laurent
  • Srinivas M. Susarla
  • Naama Samet-Rubinsteen
Original Article


Recurrent aphthous stomatitis (RAS) is a common, painful, and ulcerative disorder of the oral cavity of unknown etiology. No cure exists and medications aim to reduce pain associated with ulcers through topical applications or reduce outbreak frequency with systemic medications, many having serious side effects. The purpose of this pilot study was to evaluate the potential of a product to reduce the number of outbreaks of RAS ulcers. Propolis is a bee product used in some cultures as treatment for mouth ulcers. In this randomized, double-blind, placebo-controlled study, patients were assigned to take 500 mg of propolis or a placebo capsule daily. Subjects reported a baseline ulcer frequency and were contacted biweekly to record recurrences. Data were analyzed to determine if subjects had a decrease of 50% in outbreak frequency. The data indicated a statistically significant reduction of outbreaks in the propolis group (Fisher’s exact test, one sided, p = 0.04). Patients in the propolis group also self-reported a significant improvement in their quality of life (p = 0.03). This study has shown propolis to be effective in decreasing the number of recurrences and improve the quality of life in patients who suffer from RAS. Propolis should be evaluated further in a larger sample clinical trial.


Propolis Recurrent aphthous stomatitis Aphthous ulcers Clinical trial Evaluation study 


  1. 1.
    Banskota AH et al (2001) Recent progress in pharmacological research of propolis. Phytother Res 15:561–571PubMedCrossRefGoogle Scholar
  2. 2.
    Boyanova L et al (2003) Inhibition of Helicobacter pylori growth in vitro by Bulgarian propolis: preliminary report. J Med Microbiol 52:417–419PubMedCrossRefGoogle Scholar
  3. 3.
    Femiamo F, Gombos F, Scully C (2003) Recurrent aphthous stomatitis unresponsive to topical corticosteroids: a study of the comparative therapeutic effects of systemic prednisone and systemic sulodexide. Int J Dermatol 42:394–397CrossRefGoogle Scholar
  4. 4.
    Goadsby PJ et al (2004) Migraine—current understanding and treatment. N Engl J Med 346(4):257–270CrossRefGoogle Scholar
  5. 5.
    Harborne JB, Williams CA (2000) Advances in flavonoid research since 1992. Phytochemistry 55:481–504PubMedCrossRefGoogle Scholar
  6. 6.
    Havsteen BH (2002) The biochemistry and medical significance of the flavonoids. Pharmacol Ther 96:67–202PubMedCrossRefGoogle Scholar
  7. 7.
    Henschel R, Agathos M, Breit R (2002) Occupational contact dermatitis from propolis. Contact Dermatitis 47–52Google Scholar
  8. 8.
    Huleihel M, Ishano V (2001) Effect of propolis extract on malignant cell transformation by moloney murine sarcoma virus. Arch Virol 146:1517–1526CrossRefGoogle Scholar
  9. 9.
    Kerr AR, Ship JA (2003) Management strategies for HIV-associated aphthous stomatitis. Am J Clin Dermatol 4:669–680PubMedCrossRefGoogle Scholar
  10. 10.
    Khayyal MT (2003) A clinical pharmacological study of the potential beneficial effects of a propolis food products as an adjuvant in asthmatic patients. Fundam Clin Pharmacol 17:93–102PubMedCrossRefGoogle Scholar
  11. 11.
    Koo H et al (2002) Effects of compounds found in propolis on Streptococcus mutans growth and on glucosyltransferase activity. Antimicrob Agents Chemother 46:1302–1309PubMedCrossRefGoogle Scholar
  12. 12.
    Kujumgiev A et al (1999) Antibacterial, antifungal and antiviral activity of propolis of different geographical origin. J Ethnopharmacol 64:235–240PubMedCrossRefGoogle Scholar
  13. 13.
    LaCasa C et al (2000) Evidence for protective and antioxidant properties of rutin, a natural flavone, against ethanol Induced gastric lesions. J Ethnopharmacol 71:45–53CrossRefGoogle Scholar
  14. 14.
    Lewis DA, Shaw GP (2001) A natural flavonoid and synthetic analogues protect the gastric mucosa from aspirin-induced erosions. J Nutr Biochem 12:95–100PubMedCrossRefGoogle Scholar
  15. 15.
    Lozada-Nur F, Miranda C, Maliksi R (1994) Double blind clinical trial of 0.05% clobetasol propionate (corrected form proprionate) ointment in orabase and 0.05% flucinonide ointment in orabase in the treatment of patients with oral vesiculoerosive diseases. Oral Surg Oral Med Oral Pathol 77:598–604PubMedCrossRefGoogle Scholar
  16. 16.
    Middleton E, Kandaswami C, Theoharides TC (2000) The effect of plant flavonoids on mammalian cells: implications for inflammation, heart disease, and cancer. Pharmacol Rev 52:673–751PubMedGoogle Scholar
  17. 17.
    Natah SS et al (2004) Recurrent aphthous ulcers today: a review of the growing knowledge. Int J Oral Maxillofac Surg 33:221–234PubMedCrossRefGoogle Scholar
  18. 18.
    Revuz J, Guillaume JC, Janier M et al (1990) Crossover study of thalidomide vs. placebo in severe recurrent aphthous stomatitis. Arch Dermatol 126:923–927PubMedCrossRefGoogle Scholar
  19. 19.
    Russo A, Longo R, Vanella A (2002) Antioxidant activity of propolis: role of caffeic acid phenethyl ester and galangin. Fitoterapia 73(1):S21–S29PubMedCrossRefGoogle Scholar
  20. 20.
    Santos FA et al (2002) Antibacterial activity of Brazialian propolis and fractions against oral anaerobic bacteria. J Ethnopharmacol 80:1–7PubMedCrossRefGoogle Scholar
  21. 21.
    Scully C et al (2003) The diagnosis and management of recurrent aphthous stomatitis: a consensus approach. J Am Dent Assoc 134:200–206PubMedGoogle Scholar
  22. 22.
    Stoopler ET, Sollectio TP (2003) Recurrent aphthous stomatitis update for the general practitioner. N Y State Dent J:27–29Google Scholar

Copyright information

© Springer-Verlag 2007

Authors and Affiliations

  • Nachum Samet
    • 1
    Email author
  • Caroline Laurent
    • 1
  • Srinivas M. Susarla
    • 1
  • Naama Samet-Rubinsteen
    • 1
  1. 1.Harvard School of Dental MedicineBostonUSA

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