Skip to main content

Advertisement

SpringerLink
Log in

Histologic evaluation of effect of thiocyanate topical application on hamster cheek pouch

  • Original Article
  • Published:
Clinical Oral Investigations Aims and scope Submit manuscript

Abstract

The present study was conducted to investigate the effect of topical thiocyanate (SCN−) application on hamster cheek pouch mucosa. Sixty-six hamsters were divided into two groups. The test substance containing potassium thiocyanate was applied on the hamsters’ cheek pouches at 55 mg/kg dosages. Cheek pouches were grossly examined after 12 weeks. Histometric evaluation included height measurements from the epithelium without stratum corneum, stratum corneum, and full epithelium. Clear cells frequency was compared between groups. Results were analyzed using Student’s t test. The experimental group showed a lower height of the stratum corneum (p=0.035) and higher frequency of clear cells (p<0.001). There was no height difference for the epithelium without stratum corneum (p=0.677) and full epithelium (p=0.904) between groups. SCN− promotes no gross alterations on hamsters’ cheek pouch and does not induce dysplastic features at the epithelium or inflammation at the connective tissue. The ion promotes histologic alterations on mucosa that need to be investigated in further studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  1. Aydin HH, Çelik HA, Ersoz B (2002) Role of thiocyanate ion in metallothionein induction and in endogenous distribution of essential elements in the rat liver. Biol Trace Elem Res 90:187–202

    Article  PubMed  Google Scholar 

  2. Bánóczy J, Csiba A (1976) Occurrence of epithelial dysplasia in oral leukoplakia. Analysis and follow-up study of 12 cases. Oral Surg Oral Med Oral Pathol 42:766–774

    Article  PubMed  Google Scholar 

  3. Cherubini K (1998) Análise comparativa do ambiente bucal entre indivíduos portadores de ulceração aftosa recorrente, indivíduos fumantes e indivíduos não-fumantes. Ph.D. (MmedSci) thesis, Faculdade de Odontologia, PUCRS

  4. Glatz Z, Nováková S, Sterbová H (2001) Analysis of thiocyanate in biological fluids by capillary zone electrophoresis. J Chromatogr A 916:1–2

    Article  Google Scholar 

  5. Hoogendoorn H, Piessens JP (1987) Treatment of aphthous patients by enhancement of the salivary peroxidase system. J Oral Pathol 16:425–427

    Article  PubMed  Google Scholar 

  6. Kramer A, Weuffen W (1990) Protektive Wirkung von thiocianat am Modell der septisch infizierten Maus*. Zentralbl Hyg Umweltmed 190:293–296

    PubMed  Google Scholar 

  7. Kramer A, Weuffen W, Minnich S et al (1990) Förderung der Haarentwicklung durch Thiocyanat beim Meerschweinchen. Dermatol Monatsschr 176:417–420

    PubMed  Google Scholar 

  8. Linsdell P (2001) Thiocyanate as a probe of the cystic fibrosis transmembrane conductance regulator chloride channel pore. Can J Physiol Pharmacol 79:573–579

    Article  PubMed  Google Scholar 

  9. Luepker RV, Pechacek TF, Murray DM, Johnson A, Hund F, Jacobs DR (1981) Saliva thiocyanate: a chemical indicator of cigarette smoking in adolescents. Am J Public Health 71:1320–1324

    Article  PubMed  Google Scholar 

  10. Okafor PN, Okorowkwo CO, Maduagwu EN (2002) Occupational and dietary exposures of humans to cyanide poisoning from large-scale cassava processing and ingestion of cassava foods. Food Chem Toxicol 40:1001–1005

    Article  PubMed  Google Scholar 

  11. Rosin M, Kocher T, Kramer A (2001) Effects of SCN−/H2O2 combinations in dentifrices on plaque and gingivitis. J Clin Periodontol 28:270–276

    Article  PubMed  Google Scholar 

  12. Rosin M, Kramer A, Bradtke D, Ritcher G, Kocher T (2002) The effect of a SCN−/H2O2 toothpaste compared to a commercially available triclosan-containing toothpaste on oral hygiene and gingival health—a 6-month home-use study. J Clin Periodontol 29:1086–1091

    Article  PubMed  Google Scholar 

  13. Saji S, Morioka T, Matsumura T (1968) Salivary excretion of injected thiocyanate in hamster and its relation to the salivary peroxidase antibacterial activity. J Osaka Univ Dent Sch 8:33–37

    PubMed  Google Scholar 

  14. Sawant SS, Kandarkar SV (2000) Role of vitamins C and E as chemopreventive agents in the hamster cheek pouch treated with the oral carcinogen-DMBA. Oral Dis 6:241–247

    Article  PubMed  Google Scholar 

  15. Tommasi AF (1977) Ulceração aftosa recorrente–variação na ocorrência em função do hábito de fumar e sua intensidade. Rev Fac Odontol São Paulo 15:1–8

    PubMed  Google Scholar 

  16. Weingärtner J, Martens A, Bienengräber, Fanghänel J, Kundt G (2004) Do folic acid and thiocyanate have a preventive effect on exogenously induced disturbances of embryonic cranial development? Clin Oral Investig 8:161–164

    Article  PubMed  Google Scholar 

  17. Weuffen W, Kramer A, Thürkow B, Winetzka H (1994) Einfluss einer alimentären Thiocyanatergänzung auf Felleigenschaften beim Nerz (Mustela vision). Berl Munch Tierarztl Wochenschr 107:299–302

    PubMed  Google Scholar 

  18. World Health Organization (1997) International histological classification of tumors. Histological typing of cancer and precancer of the oral mucosa, 2nd edn. Springer, Berlin Heidelberg New York

    Google Scholar 

  19. Wood JL (1975). Biochemistry. In: Newman AA (ed) Chemistry and biochemistry of thiocyanate acid and its derivatives. Academic, New York, USA, pp 156–221

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Stomatology, São Lucas Hospital PUCRS, Porto Alegre, Brazil

    Fernanda Gonçalves Salum, Karen Cherubini, Maria Antonia Zancanaro de Figueiredo & Liliane Soares Yurgel

  2. Department of Veterinary Medicine, Universidade Federal de Pelotas, Pelotas, Brazil

    Milton de Oliveira Amado

  3. Department of Pharmacology, São Lucas Hospital PUCRS, Porto Alegre, Brazil

    Eloni Terezinha Rodrigues

  4. Hospital São Lucas, PUCRS, Avenida Ipiranga 6690, Sala 231 90610-000, Porto Alegre, Rio Grande do Sul, Brazil

    Fernanda Gonçalves Salum

  5. Stomatology and Buco-Maxilo-Facial Cancer Prevention Unit, São Lucas Hospital, Pontifícia Universidade Católica do Rio Grande do Sul (PUCRS), Porto Alegre, Brazil

    Fernanda Gonçalves Salum, Karen Cherubini, Maria Antonia Zancanaro de Figueiredo, Milton de Oliveira Amado & Eloni Terezinha Rodrigues

Authors
  1. Fernanda Gonçalves Salum
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Karen Cherubini
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Maria Antonia Zancanaro de Figueiredo
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Milton de Oliveira Amado
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Eloni Terezinha Rodrigues
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Liliane Soares Yurgel
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Fernanda Gonçalves Salum.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Salum, F.G., Cherubini, K., de Figueiredo, M.A.Z. et al. Histologic evaluation of effect of thiocyanate topical application on hamster cheek pouch. Clin Oral Invest 10, 211–215 (2006). https://doi.org/10.1007/s00784-006-0054-3

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00784-006-0054-3

Keywords

Search

Navigation