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Inhibition of enamel lesion formation by fluoridated milk assessed by laser fluorescence—an in vitro study

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Abstract

The aim of this study was to investigate the effect of fluoridated milk on enamel lesion formation as assessed by laser fluorescence (LF). The material consisted of 18 extracted premolar teeth that were cut in mesial–distal direction and pairwise assigned to either test or control samples in an experimental caries model. The teeth were exposed to a low-pH 5% cellulose gel for 4 h, 5 days per week immediately followed by a 4-h period in either fluoridated (5 ppm, test) or nonfluoridated milk (control). In the meantime, the specimens were stored in pooled human-stimulated whole saliva in room temperature. All teeth were examined by visual inspection with a magnifying glass and by LF readings (DIAGNOdent) at baseline and after 2 and 4 weeks. The baseline LF readings ranged from 3 to 7 with a mean value of 5.6±0.9. The mean values increased with time in both groups but the increase was more marked in the control teeth, 8.7±2.3 vs 12.8±3.3 after 4 weeks, this difference being statistically significant (p<0.01). The visual examination could not distinguish between the test or control samples after 2 and 4 weeks, respectively. The findings indicated that fluoride added to milk may to some extent counteract enamel lesion formation as assessed by LF in an experimental caries model.

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References

  1. Andersson A, Sköld-Larsson K, Hallgren A, Petersson LG, Twetman S (2004) Measurement of enamel lesion regression with a laser fluorescence device (DIAGNOdent): a pilot study. Orthodontics 1:201–205

    Google Scholar 

  2. Arnold WH, Cerman M, Neuhaus K, Gaengler P (2003) Volumetric assessment and quantitative element analysis of the effect of fluoridated milk on enamel demineralisation. Arch Oral Biol 48:467–473

    Article  PubMed  Google Scholar 

  3. Bánóczy J, Stephen KW, Pakhomov GN (1996) Milk fluoridation for the prevention of dental caries. In: Stephen KW, Banoczy J, Pakhomov GN (eds) Milk fluoridation for the prevention of dental caries. World Health Organization, Geneva, pp 46–71

    Google Scholar 

  4. Bowen WH, Pearson SK (1993) Effect of milk on cariogenesis. Caries Res 27:461–466

    PubMed  Google Scholar 

  5. Engström K, Petersson LG, Twetman S (2002) Fluoride concentration in supragingival dental plaque after a single intake or habitual consumption of fluoridated milk. Acta Odontol Scand 60:311–314

    Article  PubMed  Google Scholar 

  6. Engström K, Petersson LG, Sjöström I, Twetman S (2004) Composition of the salivary microflora during habitual consumption of fluoridated milk. Acta Odontol Scand 62:143–146

    Article  PubMed  Google Scholar 

  7. Engström K, Sjöström I, Petersson LG, Twetman S (2004) Lactic acid formation in supragingival dental plaque after schoolchildren’s intake of fluoridated milk. Oral Health Prev Dent 2:13–17

    PubMed  Google Scholar 

  8. Hibst R, Paulus R, Lussi A (2001) Detection of occlusal caries by laser fluorescence: Basic and clinical investigations. Med Laser Appl 16:205–213

    Article  Google Scholar 

  9. Iijiama Y, Tagaki O, Ruben J, Arends J (1999) In vitro remineralisation of in vivo and in vitro formed enamel lesions. Caries Res 33:206–213

    Article  Google Scholar 

  10. Ivancakova R, Hogan MM, Harless JD, Wefel JS (2003) Effect of fluoridated milk on progression of root surface lesions in vitro under pH cycling conditions. Caries Res 37:166–171

    Article  PubMed  Google Scholar 

  11. Ivancakova R, Harless JD, Hogan MM, Wefel JS (2005) Effect of 2% plain and fluoridated milk on root surface caries in vitro. Spec Care Dentist 25:118–123

    Article  PubMed  Google Scholar 

  12. Kahama RW, Damen JJ, ten Cate JM (1998) The effect of intrinsic fluoride in cow’s milk on in vitro enamel demineralisation. Caries Res 32:200–203

    Article  PubMed  Google Scholar 

  13. Lennon MA, Bian JY, Kolesnik AG, Kuzmina EM, Pakhomov GN, Phillips PC, Marthaler T, Villa A, Möller I (1996) Legislation and community based aspects of the implementation of a milk fluoridation programme. In: Stephen KW, Banoczy J, Pakhomov GN (eds) Milk fluoridation for the prevention of dental caries. World Health Organization, Geneva, pp 72–96

    Google Scholar 

  14. Levine RS (2001) Milk, milk flavoured products and caries. Br Dent J 191:20

    Article  PubMed  Google Scholar 

  15. Lussi A, Hibst R, Paulus R (2004) DIAGNOdent: an optical method for caries detection. J Dent Res 83:C80–C83

    Article  PubMed  Google Scholar 

  16. McDougall WA (1977) Effect of milk on enamel demineralisation and remineralisation in vitro. Caries Res 11:166–172

    PubMed  Google Scholar 

  17. Petersson LG, Derand T (1981) Development of artificial carious lesions in enamel after F-varnish (Duraphat) and F–Fe–Al-solution treatment. Swed Dent J 5:219–233

    PubMed  Google Scholar 

  18. Petersson LG, Arvidsson I, Lynch E, Engström K, Twetman S (2002) Fluoride concentration in saliva and dental plaque in young children after intake of fluoridated milk. Caries Res 36:40–43

    Article  PubMed  Google Scholar 

  19. Rugg-Gunn AJ, Boteva ES (2000) Protein mediated fluoridation and enamel remineralisation: an in vitro approach. Stoma 28:287–291

    Google Scholar 

  20. Shi XO, Traneus S, Angmar-Månson B (2001) Validation of DIAGNOdent for quantification of smooth-surface caries: an in vitro study. Acta Odontol Scand 59:74–78

    Article  PubMed  Google Scholar 

  21. Shi XO, Traneus S, Angmar-Månson B (2001) Comparison of QLF and DIAGNOdent for quantification of smooth surface caries. Caries Res 35:21–26

    Article  PubMed  Google Scholar 

  22. Sköld-Larsson K, Fornell A-C, Lussi A, Twetman S (2004) Effect of topical applications of a chlorhexidine/thymol-containing dental varnish assessed by laser fluorescence. Acta Odontol Scand 62:339–342

    Article  PubMed  Google Scholar 

  23. Tóth Z, Gintner Z, Bánóczy J, Phillips PC (1997) The effect of fluoridated milk on human dental enamel in an in vitro demineralisation model. Caries Res 31:212–215

    Article  PubMed  Google Scholar 

  24. Twetman S, Nederfors T, Petersson LG (1998) Fluoride concentration in whole saliva and separate gland secretions in schoolchildren after intake of fluoridated milk. Caries Res 32:412–416

    Article  PubMed  Google Scholar 

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Acknowledgement

This study was supported by a grant from the Borrow Foundation, Cowplain, England.

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Correspondence to L. G. Petersson.

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Engström, K., Petersson, L.G. & Twetman, S. Inhibition of enamel lesion formation by fluoridated milk assessed by laser fluorescence—an in vitro study. Clin Oral Invest 10, 249–252 (2006). https://doi.org/10.1007/s00784-006-0053-4

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  • DOI: https://doi.org/10.1007/s00784-006-0053-4

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