Abstract
The study describes the suitability of a colorimetric method (malachite green procedure) for detection of minimal amounts of phosphate (7.3–29.1 μmol/L) in different acidic solutions (hydrochloric acid, oxalic acid, maleic acid, perchloric acid, tartaric acid, citric acid, lactic acid and acetic acid) adjusted to pH 2.0. A mixture of the respective phosphate concentrations with distilled water served as control. The experiments were run with ten repeats in series. Assessment of intra- and interassay coefficient of variation and lower limit of quantification revealed that depending on the acid used, the applied method is a reliable and suitable tool to detect and quantify minimal phosphate contents in small samples of acidic solutions that have the potential to cause erosive dental lesions.
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Attin, T., Becker, K., Hannig, C. et al. Suitability of a malachite green procedure to detect minimal amounts of phosphate dissolved in acidic solutions. Clin Oral Invest 9, 203–207 (2005). https://doi.org/10.1007/s00784-005-0313-8
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DOI: https://doi.org/10.1007/s00784-005-0313-8