Abstract
Adhesion of bacteria to epithelial cells might be influenced by the degree of cell differentiation, as observed in the multi-layering process of epithelial cells. In the present study, the adhesion of a large group of clinical Porphyromonas gingivalis strains (n=11) to in vitro cultured mono- and multi-layers of epithelial cells was examined and compared. The tissue samples originated from 6 patients with chronic adult periodontitis. Porphyromonas gingivalis bacteria adhered more to mono-layers as opposed to the more differentiated multi-layers. Differences between the clinical P. gingivalis strains, however, became obvious only on multi-layers. These partially differentiated cells may also better represent the individual subject variations. Mono-layer cultures, which are simpler to obtain, seem to be less precise. The importance of cell differentiation on bacterial adhesion needs more attention.
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Papaioannou, W., van Steenberghe, D., Cassiman, JJ. et al. Adhesion of Porphyromonas gingivalis to cultured pocket epithelium: mono- and multi-layered. Clin Oral Invest 7, 162–166 (2003). https://doi.org/10.1007/s00784-003-0217-4
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DOI: https://doi.org/10.1007/s00784-003-0217-4