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Repair of critical long bone defects using frozen bone allografts coated with an rhBMP-2-retaining paste

  • Original Article
  • Published:
Journal of Orthopaedic Science

Abstract

Background

Massive frozen stocked allogeneic bone grafts are often used to reconstruct large bone defects caused by trauma or tumor resections. However, the long-term failure rate of such massive allografts was reported to be 25% because of infection, fracture, and nonunion. In this study, we evaluated the ability of a recombinant human bone morphogenetic protein (rhBMP)-2-retaining paste to promote the osteogenic potential of frozen stocked allogeneic bone grafts to repair intercalated femoral shaft defects in a rat model.

Methods

After confirming the transplantation intolerance between two rat strains (Wistar and Lewis) by skin transplantation from Lewis rats to Wistar rats, an 8-mm-long bone segment was removed from the Wistar rats, and a frozen stocked allograft coated with the rhBMP-2-retaining paste from the Lewis rats was placed into the defect and subjected to intramedullary fixation with an 18-gauge injection needle pin. The allografted femurs were evaluated by radiographic, histologic, and biomechanical examinations at specified time points.

Results

The results revealed successful repair of critical-size cortical bone defects by implanting frozen stocked allografts coated with the rhBMP-2-retaining synthetic biodegradable carrier paste from an immunologically intolerant host.

Conclusions

This experimental study suggest that allogeneic bone grafting in combination with rhBMP-2 and its local delivery system may represent an innovative approach to the reconstruction of bone defects.

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Acknowledgments

The authors would like to thank Ms K. Hata for her technical assistance.

Conflict of interest

The authors declare that they have no conflict of interest. The authors did not receive and will not receive any benefits or funding from any commercial party related directly or indirectly to the subject of this article.

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Authors and Affiliations

  1. Department of Orthopedic Surgery, Osaka City University Graduate School of Medicine, 1-4-3 Asahi-Machi, Abeno-Ku, Osaka, 545-8585, Japan

    Hiroyuki Yasuda, Koichi Yano, Shigeyuki Wakitani, Tomiya Matsumoto, Hiroaki Nakamura & Kunio Takaoka

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  1. Hiroyuki Yasuda
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  2. Koichi Yano
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  3. Shigeyuki Wakitani
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  4. Tomiya Matsumoto
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  5. Hiroaki Nakamura
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  6. Kunio Takaoka
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Corresponding author

Correspondence to Hiroyuki Yasuda.

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Yasuda, H., Yano, K., Wakitani, S. et al. Repair of critical long bone defects using frozen bone allografts coated with an rhBMP-2-retaining paste. J Orthop Sci 17, 299–307 (2012). https://doi.org/10.1007/s00776-012-0196-x

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  • DOI: https://doi.org/10.1007/s00776-012-0196-x

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