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Insights on PRAME and osteosarcoma by means of gene expression profiling

  • Original Article
  • Published:
Journal of Orthopaedic Science

Abstract

Background

Osteosarcoma (OS) is the most frequent bone tumor in children and adolescents. Tumor antigens are encoded by genes that are expressed in many types of solid tumors but are silent in normal tissues, with the exception of placenta and male germ-line cells. It has been proposed that antigen tumors are potential tumor markers.

Objectives

The premise of this study is that the identification of novel OS-associated transcripts will lead to a better understanding of the events involved in OS pathogenesis and biology.

Methods

We analyzed the expression of a panel of seven tumor antigens in OS samples to identify possible tumor markers. After selecting the tumor antigen expressed in most samples of the panel, gene expression profiling was used to identify osteosarcoma-associated molecular alterations. A microarray was employed because of its ability to accurately produce comprehensive expression profiles.

Results

PRAME was identified as the tumor antigen expressed in most OS samples; it was detected in 68% of the cases. Microarray results showed differences in expression for genes functioning in cell signaling and adhesion as well as extracellular matrix-related genes, implying that such tumors could indeed differ in regard to distinct patterns of tumorigenesis.

Conclusions

The hypothesis inferred in this study was gathered mostly from available data concerning other kinds of tumors. There is circumstantial evidence that PRAME expression might be related to distinct patterns of tumorigenesis. Further investigation is needed to validate the differential expression of genes belonging to tumorigenesis-related pathways in PRAME-positive and PRAME-negative tumors.

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Acknowledgments

This work was supported by awards from the FAPESP (The State of São Paulo Research Foundation: 04/12150-8, 07/53869-3) and GRAACC (Grupo de Apoio ao Adolescente e Criança com Câncer).

Conflict of interest

The authors report no conflicts of interest. The authors alone are responsible for the content and the writing of this paper.

Author information

Authors and Affiliations

  1. Department of Pediatrics, Pediatric Oncology Institute-GRAACC (Grupo de Apoio ao Adolescente e à Criança com Câncer)/UNIFESP (Federal University of São Paulo), Rua Botucatu no. 743, Floor 8, Genetics Laboratory, Vila Clementino, São Paulo, SP, 04023-062, Brazil

    Sílvia Regina Caminada Toledo, Indhira Dias Oliveira, Francine Tesser Gamba, Cristiane A. Dalla Torre, Maria Tereza Seixas Alves, Reynaldo J. Garcia-Filho & Antonio Sergio Petrilli

  2. Department of Morphology and Genetics, Federal University of São Paulo, São Paulo, SP, Brazil

    Sílvia Regina Caminada Toledo, Indhira Dias Oliveira, Francine Tesser Gamba & Cristiane A. Dalla Torre

  3. Department of Clinical Medicine and Center of Cell-based Therapy, Faculty of Medicine of Ribeirão Preto-University of São Paulo, Ribeirão Preto, SP, Brazil

    Marco Antonio Zago, Rodrigo Proto-Siqueira & Wilson A. Silva

  4. Department of Genetics and Evolutionary Biology, University of São Paulo, São Paulo, SP, Brazil

    Oswaldo K. Okamoto

  5. Albert Einstein Research and Education Institute, São Paulo, SP, Brazil

    Patricia Severino

  6. Genetics Department, University of São Paulo, Ribeirão Preto, SP, Brazil

    Ricardo Z. N. Vêncio & Wilson A. Silva

  7. Pediatrics Department, University of São Paulo, São Paulo, SP, Brazil

    Carlos A. Moreira-Filho

  8. Department of Pathology, Federal University of São Paulo, São Paulo, SP, Brazil

    Maria Tereza Seixas Alves

  9. Department of Orthopedic Surgery and Traumatology, Federal University of São Paulo, São Paulo, SP, Brazil

    Reynaldo J. Garcia-Filho

  10. Ludwig Institute, New York, NY, USA

    Andrew J. G. Simpson

Authors
  1. Sílvia Regina Caminada Toledo
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  2. Marco Antonio Zago
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  11. Cristiane A. Dalla Torre
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  14. Andrew J. G. Simpson
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Corresponding author

Correspondence to Sílvia Regina Caminada Toledo.

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Toledo, S.R.C., Zago, M.A., Oliveira, I.D. et al. Insights on PRAME and osteosarcoma by means of gene expression profiling. J Orthop Sci 16, 458–466 (2011). https://doi.org/10.1007/s00776-011-0106-7

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  • DOI: https://doi.org/10.1007/s00776-011-0106-7

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