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Evaluation of biodistribution by local versus systemic administration of 99mTc-labeled pamidronate

  • Original article
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Journal of Orthopaedic Science

Abstract

Background

There is an emerging interest in utilizing local and systemic administration of bisphosphonates in orthopedics. The primary objective of this study was to use 99mTc-pamidronate (99mTc-PAM) as a tool and compare bone and tissue uptake by local versus systemic administration.

Methods

99mTc-PAM was administered intravenously (i.v.), subcutaneously (s.c.) and by direct application (d.a.) on a surgically exposed and fractured femur (d.a.#f). The animals were imaged at 2 h and 24 h after administration and then killed. Organs were harvested, and their radioactivity was estimated. Specific uptake in the right femur was compared between groups, as was systemic exposure to 99mTc PAM.

Results

Bone uptake of 99mTc-PAM in the i.v. and s.c. groups was 2.2 ± 0.15 and 0.65 ± 0.07% ID/g, respectively, at the 2 h time point. Uptake by surgically exposed right femur (d.a) was 5.15 ± 0.26% ID/g, 134% higher than the femoral uptake by the i.v. method (P < 0.05). In the presence of exposed bone when the femur was fractured (d.a.#f), the uptake was 7.89 ± 0.46% ID/g, a further 50% increase (P < 0.05). The uptake of 99mTc-PAM increased after 24 h of application to 2.4 ± 0.15, 1.53 ± 0.09, 7.94 ± 0.99, and 13.2 ± 0.80% ID/g) for i.v., s.c., d.a., and d.a.#f methods, respectively. The increases in uptake for the d.a. methods were significantly higher than for the local methods at the 24-h time point (P < 0.05). Although renal uptake was comparable with the i.v. and s.c. methods (0.22 ± 0.03 and 0.22 ± 0.04% ID/g), it was significantly lower with the d.a. methods (0.05 ± 0.07 and 0.16 ± 0.07% ID/g) (P < 0.05). The corresponding urinary excretion was 55%, 45%, 36%, and 35% of the injected dose at 24 h.

Conclusions

The results indicate that the bone uptake of 99mTc-PAM was significantly higher (P = 0.001) and the kidney uptake significantly lower (P = 0.004) with the d.a. methods than with the i.v. or s.c. method. The findings indicate the need for further study into the potential of local administration of bisphosphonates in the presence of orthopedic indications.

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Authors and Affiliations

  1. Department of Nuclear Medicine, University of Macquarie, Department of Biological Science, The Children’s Hospital at Westmead and the Westmead Hospital, Westmead, Australia

    Divesh Kumar

  2. Department of Nuclear Medicine, University of Sydney, Disciple of Paediatrics and Child Health, The Children’s Hospital at Westmead and the Westmead Hospital, Westmead, NSW, 2145, Australia

    Vijay Kumar, Robert B. Howman-Giles & Effie Wong

  3. Orthopaedic Research and Biotechnology, University of Sydney, Disciple of Paediatrics and Child Health, The Children’s Hospital at Westmead, Westmead, Australia

    David G. Little

  4. Department of Biological Science, University of Macquarie Sydney, Sydney, Australia

    Sinan O. Ali

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Kumar, D., Kumar, V., Little, D. et al. Evaluation of biodistribution by local versus systemic administration of 99mTc-labeled pamidronate. J Orthop Sci 11, 512–520 (2006). https://doi.org/10.1007/s00776-006-1046-5

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  • DOI: https://doi.org/10.1007/s00776-006-1046-5

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