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Reduction potentials of blue and purple copper proteins in their unfolded states: a closer look at rack-induced coordination

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Abstract

 Cyclic voltammetry has been used to determine the reduction potentials of blue (Pseudomonas aeruginosa azurin) and purple (Thermus thermophilus CuA domain) copper proteins unfolded by guanidine hydrochloride. These Cu(II/I) potentials [456 (azurin); 453 (CuA) mV vs., NHE] are higher than those of the folded proteins. The downshift of the potential in the folded state can be accounted for by assuming that rack-induced axial coordination stabilizes Cu(II) relative to Cu(I) in a protein-encapsulated active site.

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Authors and Affiliations

  1. Beckman Institute, California Institute of Technology, Pasadena, CA 91125, USA, , , , , , US

    Pernilla Wittung-Stafshede, A. J. Di Bilio, J. R. Winkler & Harry B. Gray

  2. Department of Chemistry, Occidental College, Los Angeles, CA 90041, USA, , , , , , US

    Michael G. Hill & Ester Gomez

  3. Department of Chemistry, Biochemistry and Biophysics, Göteborg University, . P. Box 462, SE-405 30 Göteborg, Sweden e-mail: bgm@bcbp.gu.se, Fax: +46-31-7733910, , , , , , SE

    B. Göran Karlsson, Johan Leckner & B. G. Malmström

Authors
  1. Pernilla Wittung-Stafshede
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  2. Michael G. Hill
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  3. Ester Gomez
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  4. A. J. Di Bilio
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  5. B. Göran Karlsson
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  6. Johan Leckner
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  7. J. R. Winkler
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  8. Harry B. Gray
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  9. B. G. Malmström
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Additional information

Received: 3 March 1998 / Accepted: 6 April 1998

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Wittung-Stafshede, P., Hill, M., Gomez, E. et al. Reduction potentials of blue and purple copper proteins in their unfolded states: a closer look at rack-induced coordination. JBIC 3, 367–370 (1998). https://doi.org/10.1007/s007750050246

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  • DOI: https://doi.org/10.1007/s007750050246

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