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Morphological difference of Escherichia coli non-heme ferritin iron cores reconstituted in the presence and absence of inorganic phosphate

JBIC Journal of Biological Inorganic Chemistry volume 27, pages 583–594 (2022)Cite this article

Abstract

The iron core of Escherichia coli ferritin was reconstituted in the presence and absence of phosphate. The core formed in the presence of phosphate contained phosphate in amounts comparable to the iron content. The size distribution of the core was analyzed by analytical ultracentrifugation. A continuous size distribution was observed in the presence of phosphate, whereas a multimodal distribution was found in the absence of phosphate. In the presence of phosphate, the core size observed by electron microscopy was consistent with the inner diameter of ferritin. In contrast to this, clusters of several smaller particles were observed in the absence of phosphate. The small-angle X-ray scattering was measured under contrast matching conditions to obtain information on the iron core shape. A fringe was observed in the scattering profile in the presence of phosphate, but it was not observed in the absence of phosphate. Combining all results, we conclude that a hollow spherical core was formed in the presence of phosphate, while several small particles were formed within the inner cavity in the absence of phosphate.

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Abbreviations

AUC:

Analytical ultracentrifugation

CBB:

Coomassie brilliant blue

HEPES:

2-[4-(2-Hydroxyethyl)piperazin-1-yl]ethanesulfonic acid

ICP:

Inductively coupled plasma

MES:

2-(N-morpholino)ethanesulfonic acid

PAGE:

Polyacrylamide gel electrophoresis

PVDF:

Poly-vinylidene difluoride

SAXS:

Small-angle X-ray scattering

SDS:

Sodium dodecyl sulfate

TEM:

Transmission electron microscopy

UV:

Ultraviolet

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Acknowledgements

The synchrotron radiation SAXS experiments were performed at BL6A or BL10C of Photon Factory (Tsukuba, Japan) under the approval of the Photon Factory Program Advisory Committee (Proposal Nos. 2017G069, 2019G073, and 2021G089).

Funding

This work was supported in part by the Sasakawa Scientific Research Grant from The Japan Science Society to TK and JSPS KAKENHI Grant No. 21K06116 to MI.

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Authors and Affiliations

  1. Department of Biosciences, Soka University, 1-236 Tangi-machi, Hachioji, Tokyo, 192-8577, Japan

    Takumi Kuwata, Yuki Yanagida, Kazuo Fujiwara & Masamichi Ikeguchi

  2. Department of Bioinformatics, Soka University, 1-236 Tangi-machi, Hachioji, Tokyo, 192-8577, Japan

    Daisuke Sato, Kazuo Fujiwara & Masamichi Ikeguchi

  3. Glycan & Life Systems Integration Center, Soka University, 1-236 Tangi-machi, Hachioji, Tokyo, 192-8577, Japan

    Eriko Aoki

  4. Department of Physics, Meiji University, 1-1-1 Higashimita, Tama-ku, Kawasaki, 214-8571, Japan

    Hideyuki Yoshimura

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  1. Takumi Kuwata
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Correspondence to Masamichi Ikeguchi.

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Kuwata, T., Sato, D., Yanagida, Y. et al. Morphological difference of Escherichia coli non-heme ferritin iron cores reconstituted in the presence and absence of inorganic phosphate. J Biol Inorg Chem 27, 583–594 (2022). https://doi.org/10.1007/s00775-022-01952-5

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