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Antenna effect and phosphorescence spectra to find the location of drug tetracycline in bovine β-lactoglobulin A

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Abstract

A ternary system comprising of a Eu(III) complex of the drug Tetracycline hydrochloride (Eu3TC) bound to bovine β-lactoglobulin variant A (BLGA) in aqueous buffer at physiological pH (pH = 7.4) has been investigated to exploit the enhanced “antenna effect” to locate the bound drug and find the microenvironment of the binding site. Steady-state and time-resolved emission studies at room temperature as well as at 77 K have been carried out to evaluate the binding parameters in the binary system consisting of BLGA and tetracycline hydrochloride (TC). Low-temperature phosphorescence studies at 77 K of pure BLGA confirm Trp 19 to be the emitting residue, while Trp 61 is silent. Enhancement of BLGA phosphorescence emission in the ternary system at 77 K indicates that Trp 19 is very close to Eu(III) in the Eu3TC complex. The molecular docking results further confirm that TC binds close to Trp 19 in a hydrophobic domain. The results thus obtained can provide guidelines to design and synthesize target-oriented drugs as well as suitable bio-probes.

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Acknowledgements

S. G. gratefully acknowledges the Department of Science and Technology (DST), Government of India (Grant no.: SB/S1/PC-003/2013), and MBR gratefully acknowledges UGC [Minor Research Project No.F.PS-146/15-16 (ERO)] for financially supporting this work.

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Author notes

  1. Moumita Mukherjee

    Present address: Sri Aurobindo Vidyamandir, Hoogly, Chandannagar, West Bengal, India

Authors and Affiliations

  1. The Department of Chemistry, The Bhawanipur Education Society College, Kolkata, 700020, West Bengal, India

    Pinki Saha Sardar

  2. Department of Chemistry, Indian Institute of Technology, Kharagpur, 721302, India

    Pritam Roy & Swagata Dasgupta

  3. Department of Chemistry, Vidyasagar College for Women, Kolkata, 700006, West Bengal, India

    Maitrayee Basu Roy

  4. Department of Chemistry, Adamas University, Barasat, West Bengal, India

    Sanjib Ghosh

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  1. Moumita Mukherjee
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Correspondence to Maitrayee Basu Roy or Sanjib Ghosh.

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Mukherjee, M., Saha Sardar, P., Roy, P. et al. Antenna effect and phosphorescence spectra to find the location of drug tetracycline in bovine β-lactoglobulin A. J Biol Inorg Chem 23, 917–927 (2018). https://doi.org/10.1007/s00775-018-1591-3

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  • DOI: https://doi.org/10.1007/s00775-018-1591-3

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