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Extension of C. elegans lifespan using the ·NO-delivery dinitrosyl iron complexes

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Abstract

The ubiquitous and emerging physiology function of endogenous nitric oxide in vascular, myocardial, immune, and neuronal systems prompts chemists to develop a prodrug for the controlled delivery of ·NO in vivo and for the translational biomedical application. Inspired by the discovery of natural [Fe(NO)2] motif, herein, we develop the synthetic dinitrosyl iron complexes (DNICs) [Fe2(μ-SR)2(NO)4] (1) as a universal platform for the O2-triggered release of ·NO, for the regulation of ·NO-release kinetics (half-life = 0.6–27.4 h), and for the activation of physiological function of ·NO. Using C. elegans as a model organism, the ·NO-delivery DNIC 1 regulates IIS signaling pathway, AMPK signaling pathway, and mitochondrial function pathway to extend the lifespan and to delay the aging process based on the lifespan analysis, SA-βgal activity assay, and next-generation RNA sequencing analysis. This study unveils the anti-aging effect of ·NO and develops DNICs as a chemical biology probe for the continued discovery of unprecedented NO physiology.

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Acknowledgments

We gratefully acknowledge the support on confocal microscope from the Department of Bioscience Technology at Chung Yuan Christian University (Mr. Jhe-Jhih Lin) and the Department of Biological Science and Technology at National Chiao Tung University, and on ICP-MS from the Department of Biomedical Engineering and Environmental Science at National Tsing Hua University. We are indebted to the facilities of the C. elegans Core Facility Taiwan. We also thank the Ministry of Science and Technology (Taiwan) for financial support by Grant MOST 102-2113-M-033-009-MY2, MOST 103-2632-M-033-001-MY3, MOST 104-2113-M-033-005-MY2, MOST 106-2113-M-007-028-MY2. We also thank Dr. Lawrence Yu-Min Liu for valuable discussion.

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Authors and Affiliations

  1. Department of Chemistry, National Tsing Hua University, Hsinchu, 30013, Taiwan

    Hsiao-Wen Huang & Wen-Feng Liaw

  2. Institute of Biotechnology, National Tsing Hua University, Hsinchu, 30013, Taiwan

    Yen-Hung Lin & Horng-Dar Wang

  3. Department of Medical Science and Institute of Bioinformatics and Structural Biology, National Tsing Hua University, Hsinchu, 30013, Taiwan

    Min-Hsuan Lin & Yung-Jen Chuang

  4. Institute of Bioinformatics and Systems Biology, National Chiao Tung University, Hsinchu, 30013, Taiwan

    Ya-Rong Huang, Chih-Hung Chou, Hsiao-Chin Hong & Hsien-Da Huang

  5. Department of Chemistry, Chung Yuan Christian University, Taoyuan, 32023, Taiwan

    Mei-Ren Wang, Yu-Ting Tseng, Po-Chun Liao, Min-Chuan Chung, Yu-Jie Ma & Tsai-Te Lu

  6. Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, 30013, Taiwan

    Shou-Cheng Wu & Yun-Ming Wang

  7. Institute of Biomedical Engineering, National Tsing Hua University, Hsinchu, 30013, Taiwan

    Tsai-Te Lu

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Correspondence to Yung-Jen Chuang, Horng-Dar Wang, Yun-Ming Wang, Hsien-Da Huang, Tsai-Te Lu or Wen-Feng Liaw.

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Huang, HW., Lin, YH., Lin, MH. et al. Extension of C. elegans lifespan using the ·NO-delivery dinitrosyl iron complexes. J Biol Inorg Chem 23, 775–784 (2018). https://doi.org/10.1007/s00775-018-1569-1

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  • DOI: https://doi.org/10.1007/s00775-018-1569-1

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