Screening organometallic thiophene containing thiosemicarbazone ruthenium (II/III) complexes as potential anti-tumour agents
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The new ruthenium (III) complex has been synthesized and characterized by elemental analysis, FT-IR, UV–Vis, EI-Mass, EPR spectroscopy, and magnetic susceptibility measurement. Cytotoxic effects of organoruthenium (II/III) complexes 1a, 1b, and 2a, and their ligands (TSC1 and TSC2) in cultured human ovarian (A2780, SKOV-3, and OVCAR-3) and colon (DLD, CCD18Co, and Caco-2) cells have been investigated comparing reactivity of the Ru (II/III) complexes and their free TSC ligands. The complexes exhibit higher cytotoxicity in three cancer cell lines than in normal cells. The binding with CT-DNA and BSA of the all complexes were weak compared with their ligand in spite of the cellular uptake of these complexes into the cytoplasm and then nucleus while their cytotoxic effects were vice versa. All the results showed that Complex 1b has more efficient cytotoxicity on the colon cancer cells than ovarian cancer cells. However, Complex 2a is a better drug candidate especially for antitumor therapy of metastasized ovarian cancer.
KeywordsRuthenium (II/III) thiosemicarbazone Synthesis Cytotoxicity Antitumor Colon cancer
This study was supported by project number 215Z663 TUBITAK (The Scientific and Technological Research Council of Turkey), sincerely. We gratefully thank the Graduate School of Natural and Applied Sciences, Dokuz Eylül University, İzmir Biomedicine and Genome Center, Dokuz Eylül University and EBILTEM, Ege University. We also thank to Technician Birgül Kehlibar who made MTT analysis.
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