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Visualization of metallodrugs in single cells by secondary ion mass spectrometry imaging

  • Kui Wu
  • Feifei Jia
  • Wei Zheng
  • Qun Luo
  • Yao Zhao
  • Fuyi WangEmail author
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Part of the following topical collections:
  1. AsBIC8: 8th Asian Biological Inorganic Chemistry Special Issue

Abstract

Secondary ion mass spectrometry, including nanoscale secondary ion mass spectrometry (NanoSIMS) and time-of-flight secondary ion mass spectrometry (ToF–SIMS), has emerged as a powerful tool for biological imaging, especially for single cell imaging. SIMS imaging can provide information on subcellular distribution of endogenous and exogenous chemicals, including metallodrugs, from membrane through to cytoplasm and nucleus without labeling, and with high spatial resolution and chemical specificity. In this mini-review, we summarize recent progress in the field of SIMS imaging, particularly in the characterization of the subcellular distribution of metallodrugs. We anticipate that the SIMS imaging method will be widely applied to visualize subcellular distributions of drugs and drug candidates in single cells, exerting significant influence on early drug evaluation and metabolism in medicinal and pharmaceutical chemistry.

Graphical abstract

Recent progress of SIMS applications in characterizing the subcellular distributions of metallodrugs was summarized.

Keywords

Secondary ion mass spectrometry Metallodrug Visualization Single cell Mass spectrometry imaging 

Notes

Acknowledgements

We thank NSFC (Grant Nos: 21127901, 21135006, 21505141, 21575145, 91543101 and 21621062), and the 973 Program of MOST (2013CB531805) for support.

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Copyright information

© SBIC 2017

Authors and Affiliations

  • Kui Wu
    • 1
    • 2
    • 3
  • Feifei Jia
    • 1
    • 2
    • 3
  • Wei Zheng
    • 1
    • 2
    • 3
  • Qun Luo
    • 1
    • 2
    • 3
    • 4
  • Yao Zhao
    • 1
    • 2
    • 3
  • Fuyi Wang
    • 1
    • 2
    • 3
    • 4
    Email author
  1. 1.Beijing National Laboratory for Molecular SciencesBeijingPeople’s Republic of China
  2. 2.National Centre for Mass Spectrometry in BeijingBeijingPeople’s Republic of China
  3. 3.CAS Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of ChemistryChinese Academy of SciencesBeijingPeople’s Republic of China
  4. 4.University of Chinese Academy of SciencesBeijingPeople’s Republic of China

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