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Nickel induces oxidative burst, NF-κB activation and interleukin-8 production in human neutrophils

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Abstract

Many lines of evidence have suggested that oxidative stress and inflammation play a pivotal role in the toxicity of nickel salts. Considering that neutrophils are active participants in inflammatory processes, namely by producing high amounts of reactive oxygen species, the aim of the present study was to evaluate the putative activation of human neutrophils’ oxidative burst by nickel. Subsequently, the influence of nickel in the pathways leading to NADPH oxidation in neutrophils was evaluated by measuring protein kinase C (PKC) activation. The effects of nickel on neutrophils’ nuclear factor κB (NF-κB) activation and on the production of the proinflammatory mediators interleukin (IL)-1β, IL-6, IL-8 and tumour necrosis factor α were also evaluated. The results obtained showed that nickel, at concentrations that may be attained in vivo, stimulates the production of superoxide radical (O2 ·−), hydrogen peroxide (H2O2), and hypochlorous acid (HOCl) in human neutrophils in vitro, via activation of PKC. In addition, nickel was shown to activate NF-κB and to induce the production of IL-8 in these cells. These observations indicate that the sustained activation of human neutrophils by nickel may contribute for the long-term adverse effects on human health mediated by this metal.

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Abbreviations

ABAH:

4-Aminobenzoyl hydrazide

APF:

2-[6-(4′-Amino)phenoxy-3H-xanthen-3-on-9-yl]benzoic acid

DPI:

Diphenyleneiodonium chloride

EGTA:

Ethylene glycol bis(2-aminoethyl ether)-N,N,N′,N′-tetraacetic acid

Gö6983:

3-[1-[3-(Dimethylamino)propyl]-5-methoxy-1H-indol-3-yl]-4-(1H-indol-3-yl)-1H-pyrrole-2,5-dione

HEPES:

N-(2-Hydroxyethyl)piperazine-N′-ethanesulfonic acid

HRP:

Horseradish peroxidase

IKK:

IκB kinase

IL:

Interleukin

l-NAME:

N-Nitro-l-arginine methyl ester

MAPK:

Mitogen-activated protein kinase

MPO:

Myeloperoxidase

NF-κB:

Nuclear factor κB

NOS:

Nitric oxide synthase

PKC:

Protein kinase C

PMSF:

Phenylmethanesulfonyl fluoride

RNS:

Reactive nitrogen species

ROS:

Reactive oxygen species

TNF-α:

Tumour necrosis factor α

Tris:

Tris(hydroxymethyl)aminomethane

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Acknowledgments

A.G. and M.F. acknowledge the Portuguese Foundation for Science and Technology (FCT) for financial support for postdoctoral (SFRH/BPD/63179/2009) and Ph.D. (SFRH/BD/28502/2006) grants, respectively.

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Authors and Affiliations

  1. REQUIMTE, Departamento de Química, Faculdade de Farmácia, Universidade do Porto, Rua Aníbal Cunha, 164, 4099-030, Porto, Portugal

    Marisa Freitas, Ana Gomes & Eduarda Fernandes

  2. Serviço de Hematologia Clínica, Hospital Geral de Santo António, Largo Professor Abel Salazar, 4099-001, Porto, Portugal

    Graça Porto

Authors
  1. Marisa Freitas
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  2. Ana Gomes
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  3. Graça Porto
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  4. Eduarda Fernandes
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Correspondence to Eduarda Fernandes.

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Freitas, M., Gomes, A., Porto, G. et al. Nickel induces oxidative burst, NF-κB activation and interleukin-8 production in human neutrophils. J Biol Inorg Chem 15, 1275–1283 (2010). https://doi.org/10.1007/s00775-010-0685-3

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  • DOI: https://doi.org/10.1007/s00775-010-0685-3

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