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Iron–sulfur repair YtfE protein from Escherichia coli: structural characterization of the di-iron center

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Abstract

YtfE was recently shown to be a newly discovered protein required for the recovery of the activity of iron–sulfur-containing enzymes damaged by oxidative and nitrosative stress conditions. The Escherichia coli YtfE purified protein is a dimer with two iron atoms per monomer and the type and properties of the iron center were investigated by using a combination of resonance Raman and extended X-ray absorption fine structure spectroscopies. The results demonstrate that YtfE contains a non-heme dinuclear iron center having μ-oxo and μ-carboxylate bridging ligands and six histidine residues coordinating the iron ions. This is the first example of a protein from this important class of di-iron proteins to be shown to be involved in the repair of iron–sulfur centers.

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Acknowledgments

Work in the laboratory of L.M.S. was funded by FCT project POCI/SAU-IMI/56088/2004 and a FCT SFRH/BD/13756/2003 studentship to M.C.J. We thank Miguel Teixeira for helpful discussions.

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Authors and Affiliations

  1. Instituto de Tecnologia Química e Biológica, Universidade Nova de Lisboa, Av. da República (EAN), 2780-157, Oeiras, Portugal

    Smilja Todorovic, Marta C. Justino & Lígia M. Saraiva

  2. EMBL Outstation c/o DESY, Notkestrasse 85, 22603, Hamburg, Germany

    Gerd Wellenreuther & Wolfram Meyer-Klaucke

  3. Sekr. PC 14, Max-Volmer-Laboratorium für Biophysikalische Chemie, Institut für Chemie, Technische Universität Berlin, Straße des 17. Juni 135, 10623, Berlin, Germany

    Peter Hildebrandt & Daniel H. Murgida

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  1. Smilja Todorovic
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  2. Marta C. Justino
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  3. Gerd Wellenreuther
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  4. Peter Hildebrandt
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  5. Daniel H. Murgida
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  6. Wolfram Meyer-Klaucke
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  7. Lígia M. Saraiva
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Corresponding author

Correspondence to Lígia M. Saraiva.

Additional information

Smilja Todorovic and Marta C. Justino contributed equally to this work.

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Todorovic, S., Justino, M.C., Wellenreuther, G. et al. Iron–sulfur repair YtfE protein from Escherichia coli: structural characterization of the di-iron center. J Biol Inorg Chem 13, 765–770 (2008). https://doi.org/10.1007/s00775-008-0362-y

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  • DOI: https://doi.org/10.1007/s00775-008-0362-y

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