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Quantification of single-stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary electrophoresis: part I

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Abstract

The interactions between oligonucleotides and inorganic cations have been measured by capillary zone electrophoresis. With increasing concentrations of divalent cations (Ca2+, Mg2+, Mn2+ and Ni2+) in the running buffer, the migration behavior was evaluated by calculation of the binding constants. Besides these fundamental studies of binding equilibria, different buffer components, tris(hydroxymethyl)aminomethane and 3-(N-morpholino)propanesulfonic acid, have been investigated and their effects on metal ion binding quantified.

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Acknowledgements

We would like to thank Shimadzu Corporation and the University of Basel for their kind support. We also thank Yorck-Michael Neuhold for constructive discussions. Partial funding for this project was provided by Swiss National Science Foundation, grant numbers 200021-103812/1 and 20C321-101122.

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Authors and Affiliations

  1. Department of Chemistry, University of Basel, Spitalstrasse 51, 4056, Basel, Switzerland

    Alexandra R. Stettler, Valérie Chaurin, Edwin C. Constable, Catherine E. Housecroft & Maria A. Schwarz

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  1. Alexandra R. Stettler
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  2. Valérie Chaurin
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  3. Edwin C. Constable
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  4. Catherine E. Housecroft
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  5. Maria A. Schwarz
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Correspondence to Maria A. Schwarz.

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Stettler, A.R., Chaurin, V., Constable, E.C. et al. Quantification of single-stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary electrophoresis: part I. J Biol Inorg Chem 12, 194–203 (2007). https://doi.org/10.1007/s00775-006-0180-z

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  • DOI: https://doi.org/10.1007/s00775-006-0180-z

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