Skip to main content

Advertisement

Log in

Synergistic effect of genistein and zinc on bone components in the femoral-metaphyseal tissues of female rats

Journal of Bone and Mineral Metabolism Aims and scope Submit manuscript

Abstract:

The effect of genistein and zinc on bone components in rats was investigated. Femoral-metaphyseal tissues obtained from female rats (4 weeks old) were cultured for 24 h in a medium containing either vehicle or genistein (10−7–10−5 M) in the absence or presence of zinc sulfate (10−6–10−4 M) in vitro. The presence of genistein (10−6 and 10−5 M) or zinc (10−6 and 10−5 M) caused a significant increase in alkaline phosphatase activity, deoxyribonucleic acid (DNA), and calcium content in the metaphyseal tissues. These increases were significantly enhanced by the combination of each compound. The synergistic effect on bone components was seen in the combination with genistein (10−6 and 10−5 M) plus zinc (10−5 M). Such an effect was completely blocked by the presence of cycloheximide (10−6 M), an inhibitor of protein synthesis. Moreover, the oral administration of genistein (100 and 300 μg/kg body weight) or zinc sulfate (1 and 5 mg Zn/kg) to rats for 3 days caused a significant elevation of alkaline phosphatase activity, DNA, and calcium content in the femoral-metaphyseal tissues. These increases were significantly enhanced by the combination of each compound. The synergistic effect was seen in the case of genistein (100 and 300 μg/kg) plus zinc (5 mg/kg). These results demonstrate that the anabolic effect of genistein on bone components is synergistically enhanced by zinc in vitro and in vivo. This study further supports the view that the combination of nutritional factors has a potent anabolic effect on bone metabolism.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Author information

Authors and Affiliations

Authors

Additional information

Received: July 7, 1999 / Accepted: Sept. 6, 1999

About this article

Cite this article

Yamaguchi, M., Gao, Y. & Ma, Z. Synergistic effect of genistein and zinc on bone components in the femoral-metaphyseal tissues of female rats. J Bone Miner Metab 18, 77–83 (2000). https://doi.org/10.1007/s007740050015

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s007740050015

Navigation