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The crosstalk between LINC01089 and hippo pathway inhibits osteosarcoma progression

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Journal of Bone and Mineral Metabolism Aims and scope Submit manuscript

Abstract

Introduction

Osteosarcoma is the most common malignancy in children, with high morbidity worldwide. Researches indicated that long non-coding RNAs (lncRNAs) played crucial roles in various cancers. Nevertheless, study investigating lncRNA long intergenic non-protein coding RNA 1089 (LINC01089) in osteosarcoma is extremely rare. Thus, the research of LINC01089 is of great significance.

Materials and methods

qRT-PCR and western blot were done to test the expression of RNAs and proteins in osteosarcoma cells. Functional assays were carried out to evaluate biological behaviors of hFOB1.19 and osteosarcoma cells with or without LINC01089 knockdown and overexpression. In vitro and in vivo experiments in a rescue manner were performed to reveal the influences of LINC01089 and Hippo pathway on osteosarcoma cell phenotype and tumor growth.

Results

LINC01089 was down-regulated in osteosarcoma cells and overexpressing LINC01089 was validated to restrain cell growth in vitro and tumor growth in vivo. Additionally, silencing LINC01089 could exacerbate cell malignant behaviors. Correlation of LINC01089 and Hippo pathway was proved. Overexpressing LINC01089 could activate Hippo pathway to exert antitumor effects.

Conclusion

LINC01089 could restrain the progression of osteosarcoma through activating Hippo pathway.

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Acknowledgements

We appreciate the support of our experimenters.

Funding

The study was supported by the National Natural Science Foundation of China (81973871); Supported by Shanghai Key Clinical Specialty “Orthopedics and Trauma of Traditional Chinese Medicine” (shslczdzk03901).

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Author notes

  1. Hao Zhang and Lin Zhou contributed equally to this work.

Authors and Affiliations

  1. Department of Orthopedics, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai, 200000, China

    Hao Zhang, Lin Zhou, Wei Gu, Zhiqiang Li, Jun Sun & Xiaoen Wei

  2. Shi’s Center of Orthopedics and Traumatology, Shuguang Hospital Affiliated to Shanghai University of TCM, Shanghai, 200120, China

    Hao Zhang & Lin Zhou

  3. Institute of Traumatology and Orthopedics, Shanghai Academy of Traditional Chinese Medicine, Shanghai, 200120, China

    Hao Zhang

  4. Department of Oncology, Dongfang Hospital Affiliated to Beijing University of TCM, Beijing, China

    Shaopu Hu

  5. Shanghai University of TCM, Shanghai, 200032, China

    Yongjun Wang

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  1. Hao Zhang
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  2. Lin Zhou
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  4. Wei Gu
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Correspondence to Xiaoen Wei or Yongjun Wang.

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Zhang, H., Zhou, L., Hu, S. et al. The crosstalk between LINC01089 and hippo pathway inhibits osteosarcoma progression. J Bone Miner Metab 40, 890–899 (2022). https://doi.org/10.1007/s00774-022-01377-9

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  • DOI: https://doi.org/10.1007/s00774-022-01377-9

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