Skip to main content

Advertisement

SpringerLink
Log in

Preparation of chitosan–tannic acid coating and its anti-osteoclast and antibacterial activities in titanium implants

  • Original Article
  • Published:
Journal of Bone and Mineral Metabolism Aims and scope Submit manuscript

Abstract

Introduction

Bacterial infection and aseptic loosening caused by bone resorption at the implant interface are major clinical complications during bone defect implantation surgery, and surface modification of the implant to address the aforementioned problems has long been a research focus.

Materials and methods

In this paper, a chitosan (CTS)–tannic acid (TA) colloid coating with a negative charge and excellent hydrophilicity was prepared on a Ti6Al4V (TC4) surface using a layer-by-layer assembly method. The physical properties, anti-osteoclast activity, and antimicrobial activity of the coatings were investigated.

Results

The findings showed that when the pH value was 5 and the ratio of CTS:TA was 0.8, the carrying rate of TA was the best. Furthermore, the CTS–TA coating had no cytotoxicity on the morphology and proliferation of BMSCs cells and effectively inhibited the differentiation of RAW264.7 cells into osteoclasts and the proliferation of Staphylococcus aureus and Escherichia coli. With the increase in the immersion time of TC4 in CTS–TA colloid solution, the inhibitory effects will also enhance.

Conclusion

Therefore, the preparation of the CTS–TA coating provides a revolutionary technique for implant surface modification to avoid postoperative bacterial infection and aseptic loosening.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9
Fig. 10
Fig. 11
Fig. 12
Fig. 13
Fig. 14

Similar content being viewed by others

References

  1. Kobbe P, Laubach M, Hutmacher DW, Alabdulrahman H, Sellei RM, Hildebrand F (2020) Convergence of scaffold-guided bone regeneration and RIA bone grafting for the treatment of a critical-sized bone defect of the femoral shaft. Eur J Med Res 25:70

    Article  CAS  Google Scholar 

  2. Ibrahim M, Sarhan A, Yusof F, Hamdi M (2017) Biomedical materials and techniques to improve the tribological, mechanical and biomedical properties of orthopedic implants—a review article. J Alloy Compd 714:636–667

    Article  CAS  Google Scholar 

  3. Boden S (2000) Biology of lumbar spine fusion and use of bone graft substitutes: present, future, and next generation. Tissue Eng 6:383–399

    Article  CAS  Google Scholar 

  4. Agarwal S (2004) Osteolysis-basic science, incidence and diagnosis. Curr Orthop 18:220–231

    Article  Google Scholar 

  5. Florencio-Silva R, Sasso G, Cerri E, Simoes M, Cerri P (2015) Biology of bone tissue: structure, function, and factors that influence bone cells. BioMed Res Int 2015:421746

    Article  Google Scholar 

  6. Chung M, Song J, Lee J, Shin EJ, Park JH, Lee SH, Hwang JT, Choi HK (2019) Tannic acid, a novel histone acetyltransferase inhibitor, prevents non-alcoholic fatty liver disease both in vivo and in vitro model. Mol Metab 19:112–116

    Article  Google Scholar 

  7. Trisi P, Rao W, Rebaudi A (1999) A histometric comparison of smooth and rough titanium implants in human low-density jawbone. Int J Oral Maxillofac Implants 14:689–698

    CAS  PubMed  Google Scholar 

  8. Heinrich A, Dengler K, Koerner T, Haczek C, Deppe H, Stritzker B (2008) Laser-modified titanium implants for improved cell adhesion. Lasers Med Sci 23:55–58

    Article  Google Scholar 

  9. Uezono M, Takakuda K, Kikuchi M, Suzuki S, Moriyama K (2013) Hydroxyapatite/collagen nanocomposite-coated titanium rod for achieving rapid osseointegration onto bone surface. J Biomed Mater Res B Appl Biomater 101:1031–1038

    Article  Google Scholar 

  10. Schliephake H, Scharnweber D, Dard M, Rößler S, Sewing A (2002) Effect of RGD peptide coating of titanium implants on periimplant bone formation in the alveolar crest : an experimental pilot study in dogs. Clin Oral Implants Res 42:312–319

    Article  Google Scholar 

  11. Wu Y, Zhong L, Yu Z, Qi J (2019) Anti-neuroinflammatory effects of tannic acid against lipopolysaccharide-induced BV2 microglial cells via inhibition of NF-KB activation. Drug Dev Res 80:715–718

    Article  Google Scholar 

  12. Reddy D, Lee SM (2013) Application of magnetic chitosan composites for the removal of toxic metal and dyes from aqueous solutions. Adv Coll Interface Sci 201:68–93

    Article  Google Scholar 

  13. Steffi C, Shi Z, Kong CH, Wang W (2019) Bioinspired polydopamine and polyphenol tannic acid functionalized titanium suppress osteoclast differentiation: a facile and efficient strategy to regulate osteoclast activity at bone-implant interface. J R Soc Interf 16:2018–2019

    Article  Google Scholar 

  14. Yang X, Hu M, Chen S, Fang C, Cheng W, Yong T, Du C, Wang X, Hao Z, Shen M (2018) Tannic acid attenuated irradiation-induced apoptosis in megakaryocytes. Exp Cell Res 370:409–416

    Article  Google Scholar 

  15. Steffi C, Shi ZL, Kong CH, Chong SW, Wang D, Wang W (2019) Use of polyphenol tannic acid to functionalize titanium with strontium for enhancement of osteoblast differentiation and reduction of osteoclast activity. Polymers (Basel) 11:1256–1259

    Article  CAS  Google Scholar 

  16. Lee D, Lee SJ, Moon J-H, Kim J, Heo DN, Bang J, Lim H-N, Kwon IK (2018) Preparation of antibacterial chitosan membranes containing silver nanoparticles for dental barrier membrane applications. J Ind Eng Chem 66:196–202

    Article  CAS  Google Scholar 

  17. Adeli H, Khorasani M, Parvazinia M (2019) Wound dressing based on electrospun PVA/chitosan/starch nanofibrous mats: fabrication, antibacterial and cytocompatibility evaluation and in vitro healing assay. Int J Biol Macromol 122:238–254

    Article  CAS  Google Scholar 

  18. Gopalakrishnan L, Ramana LN, Sethuraman S (2014) Ellagic acid encapsulated chitosan nanoparticles as anti-hemorrhagic agent. Carbohydr Polym 111:215–221

    Article  CAS  Google Scholar 

  19. Ferris C, Casas M, Lucero MJ, Paz MD, Jiménez-Castellanos MR (2014) Synthesis and characterization of a novel chitosan-N-acetyl-homocysteine thiolactone polymer using MES buffer. Carbohyd Polym 111:125–132

    Article  CAS  Google Scholar 

  20. Fu JH, Ji J, Fan DZ, Shen JC (2010) Construction of antibacterial multilayer films containing nanosilver via layer-by-layer assembly of heparin and chitosan-silver ions complex. J Biomed Mater Res Part A 79:665–674

    Google Scholar 

  21. Costa E, Coelho M, Ilharco LM, Aguiar-Ricardo A, Hammond PT (2011) Tannic acid mediated suppression of PNIPAAm microgels thermoresponsive behavior. Macromolecules 44:612–621

    Article  CAS  Google Scholar 

  22. Aelenei N, Popa I, Novac O, Lisa G, Balaita L (2009) Tannic acid incorporation in chitosan-based microparticles and in vitro controlled release. J Mater Sci Mater Med 20:1095–1102

    Article  CAS  Google Scholar 

  23. Donlan R (2001) Biofilms and device-associated infections. Emerg Infect Dis 7:277–281

    Article  CAS  Google Scholar 

  24. Piyapakorn P, Akagi T, Hachisuka M, Onishi T, Matsuoka H, Akashi M (2013) Structural analysis of unimer nanoparticles composed of hydrophobized polys (amino acid). Macromolecules 46:6187–6194

    Article  CAS  Google Scholar 

  25. Jung BO, Kim CH, Choi KS, Lee YM, Kim JJ (1999) Preparation of amphiphilic chitosan and their antimicrobial activities. J Appl Polym Sci 72:1713–1719

    Article  CAS  Google Scholar 

  26. M. Kamachi, A. Nakamura, New macromolecular architecture and functions: proceedings of the OUMS’95 Toyonaka, Osaka, Japan, 2–5 June, 1995[M]. 1996.

  27. Ming K, Xi GC, Cheng SL, Chen GL, Xiang HM, Le JY (2008) Antibacterial mechanism of chitosan microspheres in a solid dispersing system against E. coli. Coll Surf B 65:197–202

    Article  Google Scholar 

  28. Sudarshan NR, Hoover DG (1992) Antibacterial action of chitosan. Food Biotechnol 6:257–272

    Article  CAS  Google Scholar 

  29. Guo ZY, Xing RG, Liu S, Zhong ZM, Wang XJL, Li PC (2007) The influence of the cationic of quaternized chitosan on antifungal activity. Int J Food Microbiol 118:214–217

    Article  CAS  Google Scholar 

  30. Mellegård H, Strand S, Christensen B, Granum P, Hardy S (2011) Antibacterial activity of chemically defined chitosans: influence of molecular weight, degree of acetylation and test organism. Int J Food Microbiol 148:48–54

    Article  Google Scholar 

  31. Guo ZY, Xing RG, Liu S, Zhong ZM, Wang XJL, Li PC (2008) The influence of molecular weight of quaternized chitosan on antifungal activity. Carbohydr Polym 71:694–697

    Article  CAS  Google Scholar 

  32. Li MQ, Chen XG, Liu JM, Tang WFXX (2010) Molecular weight-dependent antifungal activity and action mode of chitosan against Fulvia fulva (cooke) ciffrri. J Appl Polym Sci 119:3127–3135

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by the Key R&D Project of Sichuan Province (2018JY0552) and the National Natural Science Foundation of China (No. 51675447).

Author information

Author notes

  1. Bo Liao and Cheng Xu contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory for Advanced Technology of Materials of Ministry of Education, Tribology Research Institute, Southwest Jiaotong University, Chengdu, 610031, China

    Bo Liao, Cheng Xu, Zhenglun Wang & Wei Li

  2. Chengdu Advanced Metal Materials Industry Technology Research Institute, Chengdu, 610300, China

    Xinyu Liu & Dong Lu

Authors
  1. Bo Liao
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Cheng Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zhenglun Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Wei Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xinyu Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Dong Lu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Wei Li.

Ethics declarations

Conflict of interest

The authors declare that there is no conflict of interest.

Ethical approval

In our study, the cells used were derived from ATCC, no live animal experiments were performed, so there was no relevant ethical approval.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liao, B., Xu, C., Wang, Z. et al. Preparation of chitosan–tannic acid coating and its anti-osteoclast and antibacterial activities in titanium implants. J Bone Miner Metab 40, 402–414 (2022). https://doi.org/10.1007/s00774-022-01309-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00774-022-01309-7

Keywords

Search

Navigation