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The effect of aging on the nanostructure of murine alveolar bone and dentin

Abstract

Introduction

Alveolar bone, dentin, and cementum provide a striking example of structurally different collagen-based mineralized tissues separated only by periodontal ligament. While alveolar bone is strongly remodeled, this does not hold for dentin and cementum. However, additional dentin can be deposited on the inner surface of the pulp chamber also in older age. By investigating alveolar bone and molar of mice, the aim of our study is to detect changes in the mineral nanostructure with aging.

Materials and methods

Buccal-lingual sections of the mandible and first molar from C57BL/6 mice of three different age groups (young 5 weeks, adult 22 weeks and old 23 months) were characterized using synchrotron small and wide-angle X-ray scattering. Local average thickness and length of the apatite particles were mapped with several line scans covering the alveolar bone and the tooth.

Results

In alveolar bone, a spatial gradient was seen to develop with age with the thickest and longest particles in the distal part of the bone. The mineral particles in dentin were found to be become thicker, but then decrease of average length from adult to old animals. The mineral particle characteristics of dentin close to the pulp chamber were not only different to the rest of the tooth, but also when comparing the different age groups and even between individual animals in the same age group.

Conclusions

These results indicated that mineral particle characteristics were found to evolve differently between molar and alveolar bone as a function of age.

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Acknowledgements

The authors want to thank Birgit Schonert and Gabriele Wienskol for technical support and Stefan Siegel and Chenghao Li for their help at the µ-Spot Beamline, BESSY II (Helmholtz-Zentrum Berlin, Germany). The support of Dr. Michiaki Murakoshi, Mr. Shinsuke Kataoka, Mr. Mitsuo Kimura, Ms. Tsuguno Yamaguchi, Mr. Yasushi Koike, Ms. Yuki Ogawa and Mr. Kenji Egashira is acknowledged.

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Correspondence to Chika Akabane.

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CA, NT, and TO are employees of Lion Corporation. All other authors declare that they have no conflict of interest.

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All experimental procedures were approved by the Animal Care and Use Committee of LION Corporation (Protocol Number: 2014-246, 2015-045).

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Akabane, C., Pabisch, S., Wagermaier, W. et al. The effect of aging on the nanostructure of murine alveolar bone and dentin. J Bone Miner Metab 39, 757–768 (2021). https://doi.org/10.1007/s00774-021-01227-0

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Keywords

  • Alveolar bone
  • Dentin
  • Aging
  • X-ray scattering
  • Synchrotron radiation