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Delayed fracture healing in tetranectin-deficient mice

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Abstract

Tetranectin is a plasminogen-binding protein that enhances plasminogen activation, which has been suggested to play a role in tissue remodeling. Recently, we showed that tetranectin has a role in the wound-healing process. In this study, we investigated whether tetranectin plays a role in fracture healing. The fracture-healing process was studied using a femoral osteotomy model in tetranectin-null mice, previously generated by the authors. Radiographic imaging, micro-computed tomography (μCT), and histological analysis were used to evaluate osteotomy healing. In wild-type mice, a callus was apparent from 7 days, and most samples showed marked callus formation and rebridging of the cortices at the osteotomy site at 21 days. In contrast, in the tetranectin-null mice there was no callus formation at 7 days and much less callus formation and no bridging of cortices were observed at 21 days. At 35 days, all osteotomy sites showed clear rebridging, and secondary bone formation was achieved in wild-type mice by 42 days. In contrast, no clear rebridging or secondary bone formation was observed at 42 days in the tetranectin-null mice. Analysis using μCT at 21 days after osteotomy revealed that the callus area in tetranectin-null mice was smaller than that in wild-type mice. Histological analysis also showed that soft tissue and callus formation were smaller in the tetranectin-null mice at the early stage of the healing process after drill-hole injury. These results suggested that tetranectin could have a role in the positive regulation at the early stage of the fracture-healing process, which was reflected in the delayed fracture healing in tetranectin-deficient mice.

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Acknowledgments

The authors thank Mr. Chida and Mr. Murayama (Kureha Special Laboratory Co., Ltd., Iwaki, Japan) for their contributions and advice on μCT analysis. This work was partially supported by grants to K.I. from the Collaboration Center for Community and Industry, Sapporo Medical University.

Conflict of interest

The authors report no conflicting interests and have nothing to disclose. The authors alone are responsible for the content and writing of the paper.

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Authors and Affiliations

  1. Department of Orthopaedic Surgery, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ku, Sapporo, 060-8543, Japan

    Kousuke Iba, Yasuhisa Abe, Kumiko Kanaya, Hironori Chiba, Koichi Sasaki, Takayuki Dohke, Takuro Wada & Toshihiko Yamashita

  2. Department of Occupational Therapy, Sapporo Medical University School of Health Science, South-1, West-16, Chuo-ku, Sapporo, 060-8543, Japan

    Takako Chikenji

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  1. Kousuke Iba
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  2. Yasuhisa Abe
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  3. Takako Chikenji
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  4. Kumiko Kanaya
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  5. Hironori Chiba
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  7. Takayuki Dohke
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  8. Takuro Wada
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  9. Toshihiko Yamashita
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Correspondence to Kousuke Iba.

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Iba, K., Abe, Y., Chikenji, T. et al. Delayed fracture healing in tetranectin-deficient mice. J Bone Miner Metab 31, 399–408 (2013). https://doi.org/10.1007/s00774-013-0436-y

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  • DOI: https://doi.org/10.1007/s00774-013-0436-y

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