Journal of Bone and Mineral Metabolism

, Volume 29, Issue 3, pp 377–382 | Cite as

Proliferative and mineralogenic effects of insulin, IGF-1, and vanadate in fish osteoblast-like cells

  • Daniel M. Tiago
  • M. Leonor Cancela
  • Vincent LaizéEmail author
Short Communication


Fish have recently been recognized as a suitable model and a promising alternative to mammalian systems to study skeletogenesis. In this regard, several fish bone-derived cell lines have been developed and are being used to investigate mechanisms associated with insulin-like action of vanadium on extracellular matrix (ECM) mineralization. Although proliferative and mineralogenic effects of vanadate, insulin-like growth factor 1 (IGF-1), and insulin have recently been evaluated in a fish prechondrocyte cell line, no data are available in fish bone-forming cells, the osteoblasts. Using fish preosteoblast cells, we showed that IGF-1, but not insulin or vanadate, stimulated cell proliferation through the mitogen-activated protein kinase (MAPK) pathway, while both IGF-1 and vanadate inhibited cell differentiation/ECM mineralization through the same mechanism. Our data also indicated that the phosphatidyl inositol-3 kinase (PI-3K) pathway stimulates differentiation/ECM mineralization in osteoblasts and could represent a way to balance MAPK pathway action. The comparison of these new data obtained in fish with those available in mammals clearly evidenced a conservation of regulatory mechanisms among vertebrate bone-derived systems, although different players are involved.


Vanadium Insulin-like activity Intracellular signaling pathway In vitro mineralization Bony fish osteoblast 



D.M.T. was the recipient of a postdoctoral fellowship (BPD/45034/2008) from the Portuguese Science and Technology Foundation (Fundação para a Ciência e Tecnologia—FCT). This work was partially supported by CCMAR funds.

Supplementary material

774_2010_243_MOESM1_ESM.pdf (137 kb)
Supplementary material 1 (PDF 136 kb)
774_2010_243_MOESM2_ESM.pdf (125 kb)
Supplementary material 2 (PDF 124 kb)


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Copyright information

© The Japanese Society for Bone and Mineral Research and Springer 2010

Authors and Affiliations

  • Daniel M. Tiago
    • 1
  • M. Leonor Cancela
    • 1
  • Vincent Laizé
    • 1
    Email author
  1. 1.Centre of Marine Sciences (CCMAR)University of AlgarveFaroPortugal

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