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Proliferative and mineralogenic effects of insulin, IGF-1, and vanadate in fish osteoblast-like cells

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Abstract

Fish have recently been recognized as a suitable model and a promising alternative to mammalian systems to study skeletogenesis. In this regard, several fish bone-derived cell lines have been developed and are being used to investigate mechanisms associated with insulin-like action of vanadium on extracellular matrix (ECM) mineralization. Although proliferative and mineralogenic effects of vanadate, insulin-like growth factor 1 (IGF-1), and insulin have recently been evaluated in a fish prechondrocyte cell line, no data are available in fish bone-forming cells, the osteoblasts. Using fish preosteoblast cells, we showed that IGF-1, but not insulin or vanadate, stimulated cell proliferation through the mitogen-activated protein kinase (MAPK) pathway, while both IGF-1 and vanadate inhibited cell differentiation/ECM mineralization through the same mechanism. Our data also indicated that the phosphatidyl inositol-3 kinase (PI-3K) pathway stimulates differentiation/ECM mineralization in osteoblasts and could represent a way to balance MAPK pathway action. The comparison of these new data obtained in fish with those available in mammals clearly evidenced a conservation of regulatory mechanisms among vertebrate bone-derived systems, although different players are involved.

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References

  1. Nriagu JO (1998) History, occurrence, and uses of vanadium. In: Nriagu JO (ed) Vanadium in the environment: chemistry and biochemistry. Wiley, New York, pp 1–36

    Google Scholar 

  2. Rehder D (2003) Biological and medicinal aspects of vanadium. Inorg Chem Commun 6:604–617

    Article  CAS  Google Scholar 

  3. Crans DC, Smee JJ, Gaidamauskas E, Yang L (2004) The chemistry and biochemistry of vanadium and the biological activities exerted by vanadium compounds. Chem Rev 104:849–902

    Article  PubMed  CAS  Google Scholar 

  4. Shechter Y (1998) Insulin-like effects of vanadium: mechanisms of action, clinical and basic implications. Lett Pept Sci 5:319–322

    CAS  Google Scholar 

  5. Shisheva A, Shechter Y (1993) Role of cytosolic tyrosine kinase in mediating insulin-like actions of vanadate in rat adipocytes. J Biol Chem 268:6463–6469

    PubMed  CAS  Google Scholar 

  6. Anke M (2004) Vanadium—an element both essential and toxic to plants, animals and humans? Anal Real Acad Nac Farm 70:961–999

    CAS  Google Scholar 

  7. Barrio DA, Etcheverry SB (2006) Vanadium and bone development: putative signaling pathways. Can J Physiol Pharmacol 84:677–686

    Article  PubMed  CAS  Google Scholar 

  8. Canalis E (1985) Effect of sodium vanadate on deoxyribonucleic acid and protein synthesis in cultured rat calvariae. Endocrinology 116:855–862

    Article  PubMed  CAS  Google Scholar 

  9. Lau KH, Tanimoto H, Baylink DJ (1988) Vanadate stimulates bone cell proliferation and bone collagen synthesis in vitro. Endocrinology 123:2858–2867

    Article  PubMed  CAS  Google Scholar 

  10. Tiago DM, Laizé V, Aureliano M, Cancela ML (2008) Vanadate effects on bone metabolism: fish cell lines as an alternative to mammalian in vitro systems. In: Aureliano M (ed) Vanadium biochemistry. Research Signpost, Kerala, pp 269–283

    Google Scholar 

  11. McGonnell IM, Fowkes RC (2006) Fishing for gene function-endocrine modelling in the zebrafish. J Endocrinol 189:425–439

    Article  PubMed  CAS  Google Scholar 

  12. Ingham PW (2009) The power of the zebrafish for disease analysis. Hum Mol Genet 18:R107–R112

    Article  PubMed  CAS  Google Scholar 

  13. Fisher S, Jagadeeswaran P, Halpern ME (2003) Radiographic analysis of zebrafish skeletal defects. Dev Biol 264:64–76

    Article  PubMed  CAS  Google Scholar 

  14. Nissen RM, Amsterdam A, Hopkins N (2006) A zebrafish screen for craniofacial mutants identifies wdr68 as a highly conserved gene required for endothelin-1 expression. BMC Dev Biol 6:28

    Article  PubMed  Google Scholar 

  15. Rafael MS, Marques CL, Parameswaran V, Cancela ML, Laizé V (2010) Fish bone-derived cell lines: an alternative in vitro cell system to study bone biology. J Appl Ichthyol 26:230–234

    Article  Google Scholar 

  16. Tiago DM, Cancela ML, Aureliano M, Laizé V (2008) Vanadate proliferative and anti-mineralogenic effects are mediated by MAPK and PI-3K/Ras/Erk pathways in a fish chondrocyte cell line. FEBS Lett 582:1381–1385

    Article  PubMed  CAS  Google Scholar 

  17. Tiago DM, Laizé V, Cancela ML, Aureliano M (2008) Impairment of mineralization by metavanadate and decavanadate solutions in a fish bone-derived cell line. Cell Biol Toxicol 24:253–263

    Article  PubMed  CAS  Google Scholar 

  18. Pombinho AR, Laizé V, Molha DM, Marques SM, Cancela ML (2004) Development of two bone-derived cell lines from the marine teleost Sparus aurata; evidence for extracellular matrix mineralization and cell-type-specific expression of matrix Gla protein and osteocalcin. Cell Tissue Res 315:393–406

    Article  PubMed  CAS  Google Scholar 

  19. Chomczynski P, Sacchi N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate–phenol–chloroform extraction. Anal Biochem 162:156–159

    Article  PubMed  CAS  Google Scholar 

  20. Grey A, Chen Q, Xu X, Callon K, Cornish J (2003) Parallel phosphatidylinositol-3 kinase and p42/44 mitogen-activated protein kinase signaling pathways subserve the mitogenic and antiapoptotic actions of insulin-like growth factor I in osteoblastic cells. Endocrinology 144:4886–4893

    Article  PubMed  CAS  Google Scholar 

  21. Zhang W, Lee JC, Kumar S, Gowen M (1999) ERK pathway mediates the activation of Cdk2 in IGF-1-induced proliferation of human osteosarcoma MG-63 cells. J Bone Miner Res 14:528–535

    Article  PubMed  CAS  Google Scholar 

  22. Li SH, Guo DZ, Li B, Yin HB, Li JK, Xiang JM, Deng GZ (2008) The stimulatory effect of insulin-like growth factor-1 on the proliferation, differentiation, and mineralisation of osteoblastic cells from Holstein cattle. Vet J 179:430–436

    Article  PubMed  Google Scholar 

  23. Phornphutkul C, Wu KY, Gruppuso PA (2006) The role of insulin in chondrogenesis. Mol Cell Endocrinol 249:107–115

    PubMed  CAS  Google Scholar 

  24. Phornphutkul C, Wu KY, Yang X, Chen Q, Gruppuso PA (2004) Insulin-like growth factor-I signaling is modified during chondrocyte differentiation. J Endocrinol 183:477–486

    Article  PubMed  CAS  Google Scholar 

  25. Raucci A, Bellosta P, Grassi R, Basilico C, Mansukhani A (2008) Osteoblast proliferation or differentiation is regulated by relative strengths of opposing signaling pathways. J Cell Physiol 215:442–451

    Article  PubMed  CAS  Google Scholar 

  26. Osyczka AM, Leboy PS (2005) Bone morphogenetic protein regulation of early osteoblast genes in human marrow stromal cells is mediated by extracellular signal-regulated kinase and phosphatidylinositol 3-kinase signaling. Endocrinology 146:3428–3437

    Article  PubMed  CAS  Google Scholar 

  27. Reilly GC, Golden EB, Grasso-Knight G, Leboy PS (2005) Differential effects of ERK and p38 signaling in BMP-2 stimulated hypertrophy of cultured chick sternal chondrocytes. Cell Commun Signal 3:3

    Article  PubMed  Google Scholar 

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Acknowledgments

D.M.T. was the recipient of a postdoctoral fellowship (BPD/45034/2008) from the Portuguese Science and Technology Foundation (Fundação para a Ciência e Tecnologia—FCT). This work was partially supported by CCMAR funds.

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Authors and Affiliations

  1. Centre of Marine Sciences (CCMAR), University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal

    Daniel M. Tiago, M. Leonor Cancela & Vincent Laizé

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  1. Daniel M. Tiago
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  2. M. Leonor Cancela
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  3. Vincent Laizé
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Correspondence to Vincent Laizé.

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Tiago, D.M., Cancela, M.L. & Laizé, V. Proliferative and mineralogenic effects of insulin, IGF-1, and vanadate in fish osteoblast-like cells. J Bone Miner Metab 29, 377–382 (2011). https://doi.org/10.1007/s00774-010-0243-7

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  • DOI: https://doi.org/10.1007/s00774-010-0243-7

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