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Inorganic polyphosphate differentiates human mesenchymal stem cells into osteoblastic cells

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Abstract

The existence of inorganic polyphosphates [poly(P)] in human cells has been demonstrated. In osteoblasts, it is suggested that the concentration of cellular poly(P) is relatively high. In this study, we examined whether poly(P) accelerates the differentiation of human mesenchymal stem cells (hMSCs) from patients with osteoarthritis (OA) and rheumatoid arthritis (RA) into osteoblastic cells. Alkaline phosphatase (ALP) activity was induced by poly(P) in hMSCs from both OA and RA. In Alizarin Red S and osteocalcin EIA, there was a significant difference between the control and poly(P) group. In real-time PCR, there was a significant difference in ALP, collagen type 1A, osteocalcin, and bone sialoprotein between the control and poly(P) group. Our findings suggest that poly(P) have the potent role of differentiating hMSCs into osteoblastic cells at the early and later stages of osteoblastic differentiation.

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References

  1. Kornberg A (1995) Inorganic polyphosphate: toward making a forgotten polymer unforgettable. J Bacteriol 177:491–496

    CAS  PubMed  Google Scholar 

  2. Shiba T, Tsutsumi K, Ishige K, Noguchi T (2000) Inorganic polyphosphate and polyphosphate kinase: their novel biological functions and applications. Biochemistry (Mosc) 65:315–323

    CAS  Google Scholar 

  3. Brown MR, Kornberg A (2004) Inorganic polyphosphate in the origin and survival of species. Proc Natl Acad Sci USA 101:16085–16087

    Article  CAS  PubMed  Google Scholar 

  4. Wang L, Fraley CD, Faridi J, Kornberg A, Roth RA (2003) Inorganic polyphosphate stimulates mammalian TOR, a kinase involved in the proliferation of mammary cancer cells. Proc Natl Acad Sci USA 100:11249–11254

    Article  CAS  PubMed  Google Scholar 

  5. Smith SA, Mutch NJ, Baskar D, Rohloff P, Docampo R, Morrissey JH (2006) Polyphosphate modulates blood coagulation and fibrinolysis. Proc Natl Acad Sci USA 103:903–908

    Article  CAS  PubMed  Google Scholar 

  6. Han KY, Hong BS, Yoon YJ, Yoon CM, Kim YK, Kwon YG, Gho YS (2007) Polyphosphate blocks tumour metastasis via anti-angiogenic activity. Biochem J 406:49–55

    Article  CAS  PubMed  Google Scholar 

  7. Yamaoka M, Uematsu T, Shiba T, Matsuura T, Ono Y, Ishizuka M, Naramoto H, Takahashi M, Sugiura-Tomita M, Iguchi K, Yamashita S, Furusawa K (2008) Effect of inorganic polyphosphate in periodontitis in the elderly. Gerodontology 25:10–17

    Article  PubMed  Google Scholar 

  8. Leyhausen G, Lorenz B, Zhu H, Geurtsen W, Bohnensack R, Muller WE, Schroder HC (1998) Inorganic polyphosphate in human osteoblast-like cells. J Bone Miner Res 13:803–812

    Article  CAS  PubMed  Google Scholar 

  9. Schroder HC, Kurz L, Muller WE, Lorenz B (2000) Polyphosphate in bone. Biochemistry (Mosc) 65:296–303

    CAS  Google Scholar 

  10. Kawazoe Y, Shiba T, Nakamura R, Mizuno A, Tsutsumi K, Uematsu T, Yamaoka M, Shindoh M, Kohgo T (2004) Induction of calcification in MC3T3–E1 cells by inorganic polyphosphate. J Dent Res 83:613–618

    Article  CAS  PubMed  Google Scholar 

  11. Hacchou Y, Uematsu T, Ueda O, Usui Y, Uematsu S, Takahashi M, Uchihashi T, Kawazoe Y, Shiba T, Kurihara S, Yamaoka M, Furusawa K (2007) Inorganic polyphosphate: a possible stimulant of bone formation. J Dent Res 86:893–897

    Article  CAS  PubMed  Google Scholar 

  12. Shiba T, Nishimura D, Kawazoe Y, Onodera Y, Tsutsumi K, Nakamura R, Ohshiro M (2003) Modulation of mitogenic activity of fibroblast growth factors by inorganic polyphosphate. J Biol Chem 278:26788–26792

    Article  CAS  PubMed  Google Scholar 

  13. Morimoto D, Kuroda S, Kizawa T, Nomura K, Higuchi C, Yoshikawa H, Tomita T (2009) Equivalent osteoblastic differentiation function of human mesenchymal stem cells from rheumatoid arthritis in comparison with osteoarthritis. Rheumatology 48:643–649

    Article  CAS  PubMed  Google Scholar 

  14. Stein GS, Lian JB (1993) Molecular mechanisms mediating proliferation/differentiation interrelationships during progressive development of the osteoblast phenotype. Endocr Rev 14:424–442

    CAS  PubMed  Google Scholar 

  15. Siggelkow H, Rebenstorff K, Kurre W, Niedhart C, Engel I, Schulz H, Atkinson MJ, Hufner M (1999) Development of the osteoblast phenotype in primary human osteoblasts in culture: comparison with rat calvarial cells in osteoblast differentiation. J Cell Biochem 75:22–35

    Article  CAS  PubMed  Google Scholar 

  16. Kawazoe Y, Katoh S, Onodera Y, Kohgo T, Shindoh M, Shiba T (2008) Activation of the FGF signaling pathway and subsequent induction of mesenchymal stem cell differentiation by inorganic polyphosphate. Int J Biol Sci 4:37–47

    CAS  PubMed  Google Scholar 

  17. Zaidi M (2007) Skeletal remodeling in health and disease. Nat Med 13:791–801

    Article  CAS  PubMed  Google Scholar 

  18. Feldmann M, Brennan FM, Maini RN (1996) Rheumatoid arthritis. Cell 85:307–310

    Article  CAS  PubMed  Google Scholar 

  19. Goldring SR (2003) Pathogenesis of bone and cartilage destruction in rheumatoid arthritis. Rheumatology (Oxf) 42:ii11–ii16

    CAS  Google Scholar 

  20. Boyle WJ, Simonet WS, Lacey DL (2003) Osteoclast differentiation and activation. Nature (Lond) 423:337–342

    Article  CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Orthopedics, Osaka University Graduate School of Medicine, Yamadaoka 2-2, Suita, Osaka, 565-0871, Japan

    Daiki Morimoto, Tetsuya Tomita, Chikahisa Higuchi & Hideki Yoshikawa

  2. Regenetiss Inc., Tokyo Laboratory, Koganei, Tokyo, Japan

    Shinichi Kato & Toshikazu Shiba

  3. Division of Clinical Gene Therapy, Osaka University Graduate School of Medicine, Yamadaoka 2-2, Suita, Osaka, 565-0871, Japan

    Shoko Kuroda, Hironori Nakagami & Ryuichi Morishita

Authors
  1. Daiki Morimoto
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  2. Tetsuya Tomita
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  3. Shoko Kuroda
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  4. Chikahisa Higuchi
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  5. Shinichi Kato
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  6. Toshikazu Shiba
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  7. Hironori Nakagami
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  8. Ryuichi Morishita
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  9. Hideki Yoshikawa
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Correspondence to Tetsuya Tomita.

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Morimoto, D., Tomita, T., Kuroda, S. et al. Inorganic polyphosphate differentiates human mesenchymal stem cells into osteoblastic cells. J Bone Miner Metab 28, 418–423 (2010). https://doi.org/10.1007/s00774-010-0157-4

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  • DOI: https://doi.org/10.1007/s00774-010-0157-4

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