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The influence of ghrelin, adiponectin, and leptin on bone mineral density in healthy postmenopausal women

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Abstract

The association of body fat mass (FM) with bone mineral mass (BMC) and bone mineral density (BMD) has been attributed to a mechanical load exerted on the skeleton by FM and by the effect of different hormones. The aim of the present study was to determine whether there is a relationship between ghrelin, adiponectin, and leptin with BMC and BMD in healthy postmenopausal women (n = 88; age, 68.9 ± 6.8 years; body mass index, 27.4 ± 3.6 kg/m2). Body composition, BMC, and BMD were derived by dualenergy X-ray absorptiometry. Waist-to-hip (WHR) and waist-to-thigh (WTR) ratios were also obtained. Ghrelin was associated with total BMC (β = −0.945; P = 0.0001), total BMD (β = −0.959; P = 0.0001), lumbar spine BMD (β = −0.945; P = 0.0001), and femoral neck BMD (β = −0.957; P = 0.0001), and remained associated (P < 0.041) in different analyses that controlled for measured body composition and hormonal and insulin resistance values. However, the associations between ghrelin and measured bone mineral values were no longer significant (P > 0.149) when adjusted for body fat distribution values (WHR, WTR). Adiponectin was significantly related to total BMC (β = −0.931; P = 0.0001), total BMD (β = −0.940; P = 0.0001), lumbar spine BMD (β = −0.937; P = 0.0001), and femoral neck BMD (β = −0.940; P = 0.0001) values, and these relationships remained significant (P < 0.019) after adjusting for measured body fat, hormonal, and insulin resistance values but not when adjusted for fat-free mass (FFM; P > 0.106). In addition, significant associations of leptin with total BMC (β = 0.912; P = 0.0001), total BMD (β = 0.907; P = 0.0001), lumbar spine BMD (β = 0.899; P = 0.0001), and femoral neck BMD (β = 0.906; P = 0.0001) were found. These associations remained significant (P < 0.010) in different analyses that controlled for hormonal and insulin resistance values, but the associations between leptin and bone mineral values were no longer significant (P > 0.145) when adjusted for specific body composition values (WHR, WTR, FM, and FFM). In conclusion, it appears that the influence of plasma ghrelin, adiponectin, and leptin levels on BMC and BMD values is mediated or confounded by the specific body composition parameters in healthy postmenopausal women.

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References

  1. Felson DT, Zhang Y, Hannan MT, Anderson JJ (1993) Effects of weight and body mass index on bone mineral density in men and women: The Framingham study. J Bone Miner Res 8: 567–573

    Article  PubMed  CAS  Google Scholar 

  2. Jürimäe J, Jürimäe T (2006) Influence of insulin-like growth factor-1 and leptin on bone mineral content in healthy premenopausal women. Exp Biol Med 231:1673–1677

    Google Scholar 

  3. Harris S, Dallal GE, Dawson-Hughes B (1992) Influence of body weight on rates of change in bone density of the spine, hip, and radius in postmenopausal women. Calcif Tissue Int 50:19–23

    Article  PubMed  CAS  Google Scholar 

  4. Ravn P, Cizza G, Bjarmason NH, Thompson D, Daley M, Wasnich RD, McClung M, Hosking D, Yates AJ, Christiansen C (1999) Low body mass index is important risk factor for low bone mass and increased bone loss in early postmenopausal women. J Bone Miner Res 14:1622–1627

    Article  PubMed  CAS  Google Scholar 

  5. Reid IR (2002) Relationships among body mass, its compartments, and bone. Bone (NY) 31:547–555

    CAS  Google Scholar 

  6. Douchi T, Yamamoto S, Kuwahata R, Oki T, Yamasaki H, Nagata Y (2000) Effects of non-weight-bearing fat on bone mineral density in normal postmenopausal women: a key role for fat mass. Obstetr Gynecol 96:13–17

    Article  CAS  Google Scholar 

  7. Schott AM, Cormier C, Hans D, Favier F, Hausherr E, Dargent-Molina P, Delmas PD, Ribot C, Sebert JL, Breart G, Meunier PJ (1998) How hip and whole-body bone mineral density predict hip fracture in elderly women: The EPIDOS Prospective Study. Osteopor Int 8:247–254

    Article  CAS  Google Scholar 

  8. Rubin CT, Lanyon LE (1985) Regulation of bone mass by mechanical strain magnitude. Calcif Tissue Int 37:411–417

    Article  PubMed  CAS  Google Scholar 

  9. Blain H, Vuillemin A, Guillemin F, Durant R, Hanesse B, De Talance N, Doucet B, Jeandel C (2002) Serum leptin level is a predictor of bone mineral density in postmenopausal women. J Clin Endocrinol Metab 87:1030–1035

    Article  PubMed  CAS  Google Scholar 

  10. Jürimäe J, Rembel K, Jürimäe T, Rehand M (2005) Adiponectin is associated with bone mineral density in perimenopausal women. Horm Metab Res 37:297–302

    Article  PubMed  Google Scholar 

  11. Kontogianni MD, Dafni UG, Routsias JG, Skopouli FN (2004) Blood leptin and adiponectin as possible mediators of the relation between fat mass and BMD in perimenopausal women. J Bone Miner Res 19:546–544

    Article  PubMed  CAS  Google Scholar 

  12. Thomas T, Burguera B (2002) Is leptin the link between fat and bone mass? J Bone Miner Res 17:1563–1569

    Article  PubMed  CAS  Google Scholar 

  13. Whipple T, Sharkey N, Demers L, Williams N (2002) Leptin and the skeleton. Clin Endocrinol 57:701–711

    Article  CAS  Google Scholar 

  14. Martini G, Valenti R, Giovani S, Franci B, Campagna S, Nuti R (2001) Influence of insulin-like growth factor-1 and leptin on bone mass in healthy postmenopausal women. Bone 28:113–117

    Article  PubMed  CAS  Google Scholar 

  15. Scherer PE, Williams S, Fogliano M, Baldini C, Lodish HF (1995) A novel serum protein similar to C1q, produced exclusively in adipocytes. J Biol Chem 270:26746–26749

    Article  PubMed  CAS  Google Scholar 

  16. Weyer C, Funahashi T, Tanaka S, Hotta K, Matsuzawa Y, Pratley RE, Tataranni PA (2001) Hypoadiponectemia in obesity and type 2 diabetes: close association with insulin resistance and hyperinsulinemia. J Clin Endocrinol Metab 86:1930–1935

    Article  Google Scholar 

  17. Dietz JJ, Iglesias P (2003) The role of the novel adipocyte-derived hormone adiponectin in human disease. Eur J Endocrinol 148: 293–300

    Article  Google Scholar 

  18. Matsubara M, Maruoka S, Katayose S (2002) Inverse relationship between plasma adiponectin and leptin concentrations in normalweight and obese women. Eur J Endocrinol 147:173–180

    Article  PubMed  CAS  Google Scholar 

  19. Oh KW, Lee WY, Rhee EJ, Baek KH, Yoon KH, Kang MI, Yun EJ, Park CY, Ihm SH, Choi MG, Yoo HJ, Park SW (2005) The relationship between serum resistin, leptin, adiponectin, ghrelin levels and bone mineral density in middle-aged men. Clin Endocrinol 63:131–138

    Article  CAS  Google Scholar 

  20. Jürimäe J, Jürimäe T (2007) Adiponectin is a predictor of bone mineral density in middle-aged premenopausal women. Osteoporos Int 18:1253–1259

    Article  PubMed  CAS  Google Scholar 

  21. Misra M, Miller KK, Stewart V, Hunter E, Kuo K, Herzog DB, Klibanski A (2005) Ghrelin and bone metabolism in adolescent girls with anorexia nervosa and healthy adolescents. J Clin Endocrinol Metab 90:5082–5087

    Article  PubMed  CAS  Google Scholar 

  22. Kojima M, Hosoda H, Date Y, Nakazato M, Matsuo H, Kangawa K (1999) Ghrelin is a growth-hormone-releasing acylated peptide from stomach. Nature (Lond) 402:656–660

    Article  CAS  Google Scholar 

  23. Wren AM, Small CJ, Ward HJ, Murphy KG, Dakin CL, Taheri S, Kennedy AR, Roberts GH, Morgan DG, Ghatei MA, Bloom SR (2000) The novel hypothalamic peptide ghrelin stimulates food intake and growth hormone secretion. Endocrinology 141:4325–4328

    Article  PubMed  CAS  Google Scholar 

  24. Nakazato M, Murakami N, Date Y, Kojima M, Matsuo H, Kangawa K, Matsukura S (2001) A role for ghrelin in the central regulation of feeding. Nature (Lond) 409:194–198

    Article  CAS  Google Scholar 

  25. Tschop M, Wawarta R, Riepl RL, Friedrich S, Biedlingmaier M, Landgraf R, Folwaczny C (2001) Post-prandial decrease of circulating human ghrelin levels. J Endocrinol Invest 24:RC19–RC21

    PubMed  CAS  Google Scholar 

  26. Haqq AM, Farooqi IS, O’Rahilly S, Stadler DD, Rosenfeld RG, Pratt KL, LaFranchi SH, Purnell JQ (2003) Serum ghrelin levels are inversely correlated with body mass index, age, and insulin concentrations in normal children and are markedly increased in Prader-Willi syndrome. J Clin Endocrinol Metab 88: 174–178

    Article  PubMed  CAS  Google Scholar 

  27. Vilarrasa N, Vendrell J, Maravall J, Broch M, Estepa A, Megia A, Soler J, Simon I, Richart C, Gomez JM (2005) Distribution and determinants of adiponectin, resistin and ghrelin in a randomly selected healthy population. Clin Endocrinol 63:329–335

    Article  CAS  Google Scholar 

  28. Bouchard C, Tremblay A, Leblanc C, Lortie G, Savard R, Theriault G (1983) A method to assess energy expenditure in children and adults. Am J Clin Nutr 37:461–467

    PubMed  CAS  Google Scholar 

  29. Lenchik L, Register TC, Hsu FC, Lohman K, Nicklas BJ, Freidman BI, Langefeld CD, Carr JJ, Bowden DW (2003) Adiponectin as a novel determinant of bone mineral density and visceral fat. Bone (NY) 33:646–651

    CAS  Google Scholar 

  30. Garnett SP, Högler W, Blades B, Baur LA, Peat J, Lee J, Cowell CT (2004) Relation between hormones and body composition, including bone, in prepubertal children. Am J Clin Nutr 80: 966–972

    PubMed  CAS  Google Scholar 

  31. Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF, Turner RC (1985) Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in men. Diabetologia 28:412–419

    Article  PubMed  CAS  Google Scholar 

  32. Morberg CM, Tetens I, Black E, Toubro S, Sorensen TI, Pedersen O, Astrup A (2003) Leptin and bone mineral density: a crosssectional study in obese and nonobese men. J Clin Endocrinol Metab 88:5795–5800

    Article  PubMed  CAS  Google Scholar 

  33. Zhong N, Wu XP, Xu ZR, Wang AH, Luo XH, Cao XZ, Xie H, Shan PF, Liao EY (2005) Relationship of serum leptin with age, body weight, body mass index, and bone mineral density in healthy mainland Chinese women. Clin Chim Acta 351:161–168

    Article  PubMed  CAS  Google Scholar 

  34. Sahin G, Polat G, Baethis S, Milcan A, Baethdatoethlu O, Erdoethan C, Camdeviren H (2003) Body composition, bone mineral density, and circulating leptin levels in postmenopausal Turkish women. Rheumatol Int 23:87–91

    PubMed  CAS  Google Scholar 

  35. Yilmazi M, Keles I, Aydin G, Orkun S, Bayram M, Sevine FC, Kisa U, Yetkin I (2005) Plasma leptin concentrations in postmenopausal women with osteoporosis. Endocrinol Res 31:133–138

    Article  CAS  Google Scholar 

  36. Kirchengast S, Peterson B, Hauser G, Knogler W (2001) Body composition characteristics are associated with the bone density of the proximal femur end in middle-and old-aged women and men. Maturitas 39:133–145

    Article  PubMed  CAS  Google Scholar 

  37. Jürimäe J, Jürimäe T (2007) Plasma adiponectin concentration in healthy pre-and postmenopausal women: relationship with body composition, bone mineral, and metabolic variables. Am J Physiol Endocrinol Metab 293:E42–E47

    Article  PubMed  CAS  Google Scholar 

  38. Lee WY, Rhee EJ, Oh KW, Kim SY, Jung CH, Yun EJ, Bae KH, Kang MI, Kim SW (2006) Identification of adiponectin and its receptors in human osteoplast-like cells and association of T45G polymorphism in exon 2 of adiponectin gene with lumbar spine bone mineral density in Korean women. Clin Endocrinol 65:631–637

    Article  CAS  Google Scholar 

  39. Berner HS, Lyngstadaas SP, Spahr A, Monjo M, Thommesen L, Drevon CA, Syversen U, Reseland JE (2004) Adiponectin and its receptors are expressed in bone-forming cells. Bone (NY) 35: 842–849

    CAS  Google Scholar 

  40. Shinoda Y, Yamaguchi M, Ogata N, Akune T, Kubota N, Yamauchi T, Terauchi Y, Kadowaki T, Takeuchi Y, Fukumoto S, Ikeda T, Hoshi K, Chung UI, Nakamura K, Kawaguchi H (2006) Regulation of bone formation by adiponectin through autocrine/ paracrine and endocrine pathways. J Cell Biochem 99:196–208

    Article  PubMed  CAS  Google Scholar 

  41. Oshima K, Nampei A, Matsuda M, Iwaki M, Fukuhara A, Hashimoto J, Yoshikawa H, Shimomura I (2005) Adiponectin increases bone mass by suppressing osteoclast and activating osteoblast. Biochem Biophys Res Commun 331:520–526

    Article  PubMed  CAS  Google Scholar 

  42. Weiss LA, Langenberg C, Barret-Connor E (2006) Ghrelin and bone: is there an association for older adults?: the Rancho Bernardo study. J Bone Miner Res 21:752–757

    Article  PubMed  CAS  Google Scholar 

  43. Thompson NM, Gill DA, Davies R, Loveridge N, Houston PA, Robinson IC, Wells T (2004) Ghrelin and des-octynol ghrelin promote adipogenesis directly in vivo by a mechanism independent of the type 1a growth hormone secretagogue receptor. Endocrinology 145:234–242

    Article  PubMed  CAS  Google Scholar 

  44. Caminos J, Gualillo O, Lago F, Otero M, Blanco M, Gallego R, Garcia-Caballero T, Goldring M, Casanueva F, Gomez-Reino J, Dieguez C (2005) The endogeneous growth hormone secretagogue (ghrelin) is synthesized and secreted by chondrocytes. Endocrinology 146:1285–1292

    Article  PubMed  CAS  Google Scholar 

  45. Maccarinelli G, Sibilia V, Torsello A, Raimondo F, Pitto M, Giustina A, Netti C, Cocchi D (2005) Ghrelin regulates proliferation and differentation of osteoblastic cells. J Endocrinol 184: 249–256

    Article  PubMed  CAS  Google Scholar 

  46. Fukushima N, Hanada R, Teranishi H, Fukue Y, Tachibana T, Ishikawa H, Takeda S, Takeuchi Y, Fukumoto S, Kangawa K, Nagata K, Kojima M (2005) Ghrelin directly regulates bone formation. J Bone Miner Res 20:790–798

    Article  PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Institute of Sport Pedagogy and Coaching Sciences, Centre of Behavioural and Health Sciences, University of Tartu, 18. Ülikooli St., Tartu, 50090, Estonia

    Jaak Jürimäe, Toivo Jürimäe & Aire Leppik

  2. Institute of Exercise Physiology and Physiotherapy, Centre of Behavioural and Health Sciences, University of Tartu, Tartu, Estonia

    Tatjana Kums

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  1. Jaak Jürimäe
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  2. Toivo Jürimäe
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Correspondence to Jaak Jürimäe.

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Jürimäe, J., Jürimäe, T., Leppik, A. et al. The influence of ghrelin, adiponectin, and leptin on bone mineral density in healthy postmenopausal women. J Bone Miner Metab 26, 618–623 (2008). https://doi.org/10.1007/s00774-008-0861-5

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  • DOI: https://doi.org/10.1007/s00774-008-0861-5

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