Journal of Bone and Mineral Metabolism

, Volume 25, Issue 5, pp 310–319 | Cite as

Association analysis of the polymorphisms of the VDR gene with bone mineral density and the occurrence of fractures

  • Wanda Horst-Sikorska
  • Robert Kalak
  • Anna Wawrzyniak
  • Michalina Marcinkowska
  • Liliana Celczynska-Bajew
  • Ryszard Slomski
ORIGINAL ARTICLE

Abstract

Associations of the FokI, BsmI, ApaI, and TaqI polymorphisms of the vitamin D receptor (VDR) gene with the bone mineral density (BMD) of the lumbar part of the spinal column (BMD LS) and the neck of the femur (BMD FN), and with the occurrence of fractures, were studied using the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis on DNA isolated from peripheral blood of 239 women and 40 men from the region of western Poland. Three polymorphisms of the 3′ end of the VDR gene (BsmI, ApaI, TaqI) indicated a strong linkage disequilibrium. Association analysis of the VDR gene FokI polymorphism with BMD LS showed a dose effect of allele f. The association of the bAT haplotype of the BsmI, ApaI, and TaqI polymorphisms of the VDR gene with BMD FN was statistically significant. The association of the ApaI polymorphism with the occurrence of fractures was observed. Associations were also observed between the occurrence of fractures and the baT haplotypes of the VDR gene.

Key words

osteoporosis vitamin D receptor (VDR) gene association haplotype bone mineral density 

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Copyright information

© Springer-Verlag Tokyo 2007

Authors and Affiliations

  • Wanda Horst-Sikorska
    • 1
  • Robert Kalak
    • 2
    • 3
  • Anna Wawrzyniak
    • 1
  • Michalina Marcinkowska
    • 1
  • Liliana Celczynska-Bajew
    • 1
  • Ryszard Slomski
    • 3
  1. 1.Department of Family MedicineUniversity of Medical SciencesPoznanPoland
  2. 2.Department of Biochemistry and BiotechnologyAgricultural UniversityPoznanPoland
  3. 3.Institute of Human GeneticsPolish Academy of SciencesPoznanPoland

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