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Expression of MT1-MMP during deciduous tooth resorption in odontoclasts

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Abstract

Membrane type 1-matrix metalloproteinase (MT1-MMP) is a membrane-bound matrix metalloproteinase capable of mediating pericellular proteolysis of extracellular matrix components. In osteoclasts, the localization of MT1-MMP has been reported at the tips of specialized membrane protrusions (podosomes and lamellipodia) so that osteoclasts might use MT1-MMP to perform focal proteolysis and move through the extracellular matrix to the bone surface. The objectives of this study were to investigate an association of MT1-MMP in physiological root resorption of the deciduous tooth by reverse transcriptase-polymerase chain reaction (RT-PCR) and Northern blot analysis, and to identify MT1-MMP-producing cell during deciduous tooth resorption by in situ hybridization and immunohistochemistry. RT-PCR and Northern blot analysis revealed the exclusively high expression of MT1-MMP mRNA in bovine root-resorbing tissue, which lies between the root of the deciduous tooth and its permanent successor. Expression of MT1-MMP mRNA was seen in odontoclasts aligning in the surface layer of the root-resorbing tissue at sites of root resorption. Furthermore, immmunohistochemistry also confirmed the localization of MT1-MMP protein to the odontoclasts. The present identification of MT1-MMP in odontoclasts during deciduous tooth resorption might be relevant to the migration activity that these cells have to gain access to the root surface.

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Authors and Affiliations

  1. Department of Pediatric Dentistry, Faculty of Dentistry, Chulalongkorn University, Bangkok, Thailand

    Busayarat Linsuwanont-Santiwong

  2. Developmental Oral Health Science, Department of Orofacial Development and Function, Division of Oral Health Sciences, Graduate School, Tokyo Medical and Dental University, Tokyo, Japan

    Yuzo Takagi

  3. Pharmacology, Department of Hard Tissue Engineering, Division of Bio-Matrix, Graduate School, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo, 113-8549, Japan

    Keiichi Ohya & Hitoyata Shimokawa

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  1. Busayarat Linsuwanont-Santiwong
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  2. Yuzo Takagi
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  3. Keiichi Ohya
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  4. Hitoyata Shimokawa
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Correspondence to Hitoyata Shimokawa.

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Linsuwanont-Santiwong, B., Takagi, Y., Ohya, K. et al. Expression of MT1-MMP during deciduous tooth resorption in odontoclasts. J Bone Miner Metab 24, 447–453 (2006). https://doi.org/10.1007/s00774-006-0714-z

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