Abstract
In this paper, shock sterilization using the motions of microbubbles induced by underwater shock waves is verified experimentally. A bio-experiment is carried out using marine Vibrio sp.. Underwater shock waves are produced by electric discharge in a semi-ellipsoidal discharge reflector. The shock waves are focused to increase the pressure that the generated microbubbles are exposed to. The microbubbles are generated independently. The microbubble generator can produce microbubbles of around 50 μm diameter by means of Kelvin–Helmholz instability and Venturi effect. Propagation behavior of shock waves and generation process of microbubbles are captured by high-speed camera. The experimental results show that the supply of microbubbles increases the potential of shock sterilization. In addition, it is found that shock waves without microbubbles also have the capacity of sterilization, and this means that cavitation bubbles generated behind converging shock waves contribute to inactivating marine bacteria.
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References
International Maritime Organization (2004) Adoption of international convention for the control and management of ships’ ballast water and sediments. In: International Conference on Ballast Water Management for Ships, Agenda Item 8, 16 February 2004
Tsolaki E, Aiamadopoulos E (2010) Technologies for ballast water treatment: a review. J Chem Technol Biotechnol 85:19–32
Abe A, Mimura H, Ishida H, Yoshida K (2007) The effect of shock pressures on the inactivation of a marine Vibrio sp. Shock Waves 17:143–151
Abe A (2010) Pressure generation from micro-bubble collapse at shock wave loading. J Fluid Sci Technol 6:235–246
Wang J, Abe A (2015) A hybrid analytical model of sterilization effect on marine bacteria using microbubbles interacting with shock wave. J Mar Sci Technol. doi:10.1007/s00773-015-0360-z
Xu Q, Nakajima M, Liu Z, Shiina T (2011) Biosurfactants for microbubble preparation and application. Int J Mol Sci 12:462–475
Takahashi M, Chiba K, Li P (2006) Free-Radical generation from collapsing microbubbles in the absence of a dynamic stimulus. J Phys Chem B 111:1343–1347
Kaufmann Beat A, Lindner Jonathan R (2007) Molecular imaging with targeted contrast ultrasound. Curr Option Biotechnol 18:11–16
Morawski Anne M, Lanza Gregory A, Wickline Samuel A (2005) Targeted contrast agents for magnetic resonance imaging and ultrasound. Curr Option Biotechnol 16:89–92
Chu Libing, Xing Xinhui, Anfeng Yu, Zhou Yunan, Sun Xulin, Jurcik Benjamin (2005) Enhanced ozonation of simulated dyestuff wastewater by microbubbles. Curr Option Biotechnol 16:89–92
Nobuhito Tsujii, Biyu Wan, Haruo Mimara, Akihisa Abe (2012) Experimental study on inactivation of marine bacteria using electrodischarge shock wave. In: 28th International Symposium on Shock Waves vol 2, pp 915–921
Hasegawa Hiroaki, Masaki Yasuhiro, Matsuuchi Kazuo, Yoshida Yusuke (2006) Microbubble generation by using pipe with slits. Jpn Soc Mech Eng 72:160–166
Miles JW (1959) On the Generation of surface waves by shear flows part 3. kelvin-helmhotz instability. J Fluid Mech 6:583–598
Abe A, Wang J, Shioda M, Maeno A (2014) Observation and analysis of interactive phenomena between microbubble and underwater shock wave. J Visual. doi:10.1007/s12650-014-0257-7
Koita Taketashi, Sun Mingyu (2014) Visualization of underwater shock wave and bubble phenomena induced by electric discharge in a narrow water tank. In: Proceedings of Shock Waves Symposium pp 134–135
Acknowledgments
This work was supported by JSPS KAKENHI Grant Number 25630405. The authors would like to express their thanks to Prof. H. Mimura of Kobe University for his assistance with the bio-experiment.
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Wang, J., Abe, A. Experimental verification of shock sterilization for marine Vibrio sp. using microbubbles interacting with underwater shock waves. J Mar Sci Technol 21, 679–688 (2016). https://doi.org/10.1007/s00773-016-0384-z
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DOI: https://doi.org/10.1007/s00773-016-0384-z