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LC–MS/MS quantification of ractopamine in bovine and swine muscle: stability of matrix-matched calibration solutions

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Abstract

Ractopamine is used as an animal feed additive, but its adverse effects led to the necessity of the current monitoring programs of ractopamine residues in food products, so its reliable quantification is indispensable. This work aims to demonstrate the stability of solutions of ractopamine used for calibration of a liquid chromatography–tandem mass spectrometry method. Naturally contaminated samples with ractopamine were quantified using solutions prepared on different days. The stability study was based on an empirical model that considered a straight line (slope = 1) to describe the degradation mechanism. No significant change in the slope was detected. It could be concluded that solutions underpinning calibration curves at two different working intervals were stable for at least 11 months and 5 months, respectively.

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Authors and Affiliations

  1. Laboratório de Resíduos de Medicamentos Veterinários, Laboratório Nacional Agropecuário (LANAGRO), Rômulo Joviano Avenue, Pedro Leopoldo, MG, 33600-000, Brazil

    Leila Rodrigues Caldeira, Josefa Abucáter Lima, Flávio Alves Santos, Mary Ane Gonçalves Lana & Ana Cláudia dos Santos Rossi Ribeiro

  2. Post-Graduation Metrology Programme, Pontifical Catholic University of Rio de Janeiro, Rio de Janeiro, RJ, 22453-900, Brazil

    Elcio Cruz de Oliveira

  3. Petrobras Transporte S.A. – Transpetro, Technology Management, São Bento Street, 29, Rio de Janeiro, RJ, 20090-010, Brazil

    Elcio Cruz de Oliveira

Authors
  1. Leila Rodrigues Caldeira
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  2. Josefa Abucáter Lima
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  3. Flávio Alves Santos
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  4. Mary Ane Gonçalves Lana
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  5. Ana Cláudia dos Santos Rossi Ribeiro
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  6. Elcio Cruz de Oliveira
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Correspondence to Elcio Cruz de Oliveira.

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Caldeira, L.R., Lima, J.A., Santos, F.A. et al. LC–MS/MS quantification of ractopamine in bovine and swine muscle: stability of matrix-matched calibration solutions. Accred Qual Assur 22, 91–96 (2017). https://doi.org/10.1007/s00769-017-1255-y

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  • DOI: https://doi.org/10.1007/s00769-017-1255-y

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