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In-house validation of a UV spectrometric method for measurement of nitrate concentration in natural groundwater samples


This paper presents the validation and evaluation of measurement uncertainty of a method for quantitative measurement of nitrate concentration in various groundwater samples by means of molecular UV absorption spectrometry. A calibration study was conducted for the working range from 0.10 mg/L to 6.00 mg/L NO3 , being accordingly determined detection and quantification limits of 0.07 mg/L NO3 and 0.10 mg/L NO3 , respectively. It was also proven that the method is accurate and precise. Selectivity and applicability were discussed. Measurement uncertainty was evaluated based on the validation data.

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This work was supported by a Grant of the Romanian National Authority for Scientific Research, CNCS–UEFISCDI, project number PN–RU–PD–2011–3–0081.

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Correspondence to Alin Tudorache.

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Tudorache, A., Ioniţă, D.E., Marin, N.M. et al. In-house validation of a UV spectrometric method for measurement of nitrate concentration in natural groundwater samples. Accred Qual Assur 22, 29–35 (2017).

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  • Nitrate measurement
  • UV spectrometry
  • Groundwater
  • Validation
  • Measurement uncertainty