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In-house validation of a UV spectrometric method for measurement of nitrate concentration in natural groundwater samples

Abstract

This paper presents the validation and evaluation of measurement uncertainty of a method for quantitative measurement of nitrate concentration in various groundwater samples by means of molecular UV absorption spectrometry. A calibration study was conducted for the working range from 0.10 mg/L to 6.00 mg/L NO3 , being accordingly determined detection and quantification limits of 0.07 mg/L NO3 and 0.10 mg/L NO3 , respectively. It was also proven that the method is accurate and precise. Selectivity and applicability were discussed. Measurement uncertainty was evaluated based on the validation data.

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References

  1. 1.

    Spalding RF, Exner ME (1993) Occurrence of nitrate in groundwater—a review. J Environ Qual 22:392–402

    CAS  Article  Google Scholar 

  2. 2.

    Fan AM, Willhite CC, Book SA (1987) Evaluation of the nitrate drinking water standard with reference to infant methemoglobinemia and potential reproductive toxicity. Regul Toxicol Pharm 7:135–148

    CAS  Article  Google Scholar 

  3. 3.

    Moorcroft MJ, Davis J, Compton RG (2001) Detection and determination of nitrate and nitrite: a review. Talanta 54:785–803

    CAS  Article  Google Scholar 

  4. 4.

    Ito K, Takayama Y, Makabe N, Mitsui R, Hirokawa T (2005) Ion chromatography for measurement of nitrite and nitrate in seawater using monolithic ODS columns. J Chromatogr A 1083:63–67

    CAS  Article  Google Scholar 

  5. 5.

    Zhang J-Z, Fischer CJ (2006) A simplified resorcinol method for direct spectrophotometric measurement of nitrate in seawater. Mar Chem 99:220–226

    CAS  Article  Google Scholar 

  6. 6.

    ISO 7890-3 (1988) Water quality—measurement of nitrate—part 3: spectrometric method using sulfosalicylic acid. International Organization for Standardization (ISO), Geneva

    Google Scholar 

  7. 7.

    APHA Method 4500-NO3-E (1992) Standard methods for the examination of water and wastewater. American public Health Association, Washington, D.C

    Google Scholar 

  8. 8.

    APHA Method 4500-NO3-F (1992) Standard methods for the examination of water and wastewater. American public Health Association, Washington, D.C

    Google Scholar 

  9. 9.

    Brown L, Bellinger EG (1978) Nitrate measurement in fresh and some estuarine waters by ultraviolet light absorption: a new proposed method. Water Res 12:223–229

    CAS  Article  Google Scholar 

  10. 10.

    Drolc A, Vrtovšek J (2010) Nitrate and nitrite nitrogen measurement in waste water using on-line UV spectrometric method. Bioresour Technol 101:4228–4233

    CAS  Article  Google Scholar 

  11. 11.

    Rennie PJ, Sumner AM (1979) Measurement of nitrate in raw, potable and waste waters by ultraviolet spectrophotometry. Analyst 104:837–845

    Article  Google Scholar 

  12. 12.

    Edwards AC, Hooda PS, Cook Y (2001) Measurement of nitrate in water containing dissolved organic carbon by ultraviolet spectroscopy. Int J Environ Anal Chem 80:49–59

    CAS  Article  Google Scholar 

  13. 13.

    Sah RN (1994) Nitrate–nitrogen determination—a critical review. Commun Soil Sci Plant Anal 25:2841–2869

    Article  Google Scholar 

  14. 14.

    Greenberg AE, Clesceri LS, Eaton AD (1992) Standard methods for the examination of water and wastewater. American Public Health Association, Washington

    Google Scholar 

  15. 15.

    Pham HN, Benitez A, Hommet F, Bombe D, Schoefs O, Pauss A (2008) A new quantitative and low-cost determination method of nitrate in vegetables, based on deconvolution of UV spectra. Talanta 76:936–940

    Article  Google Scholar 

  16. 16.

    Hibbert B (1999) Method validation of modern analytical techniques. Accred Qual Assur 4:352–356

    Article  Google Scholar 

  17. 17.

    ISO/IEC 17025 (2005) General requirements for the competence of testing and calibration laboratories. International Organization for Standardization (ISO), Geneva

  18. 18.

    Stefan RI, Baiulescu GE, Aboul-Enein HY, van Staden JF (1999) Estimation of uncertainties in clinical analysis. Accred Qual Assur 4:225–229

    Article  Google Scholar 

  19. 19.

    Huang H (2014) Uncertainty-based measurement quality control. Accred Qual Assur 19:65–73

    Article  Google Scholar 

  20. 20.

    Joint Committee for Guides in Metrology (JCGM) (2008) Evaluation of measurement data-guide to the expression of uncertainty in measurement (GUM 1995 with minor corrections) Sèvres, France. http://www.bipm.org/utils/common/documents/jcgm/JCGM_100_2008_E.pdf

  21. 21.

    ISO 11352 (2012) Water quality-estimation of measurement uncertainty based on validation and quality control data. International Organization for Standardization (ISO), Geneva

    Google Scholar 

  22. 22.

    GBC Scientific Application Notes-UV visible (1998) Fully automated water nitrate analysis. http://www.gbcscientific.com/appnotes/uv_app_note_007.pdf

  23. 23.

    ICH Q2 (2005) Validation of analytical procedures: text and methodology. http://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Quality/Q2_R1/Step4/Q2_R1__Guideline.pdf

  24. 24.

    Council Directive 98/83/EC (1998) On the quality of water intended for human consumption, The Council of the European Union

  25. 25.

    Senila M, Drolc A, Pintar A, Senila L, Levei E (2014) Validation and measurement uncertainty evaluation of the ICP-OES method for the multi-elemental measurement of essential and nonessential elements from medicinal plants and their aqueous extracts. J Anal Sci Technol 5:1–9

    Article  Google Scholar 

  26. 26.

    Tanase IGH, Popa DE, David IG, Buleandra M (2010) Single laboratory validation of a method for the measurement of total inorganic arsenic by hydride generation atomic absorption spectrometry. Anal Lett 43:1172–1189

    CAS  Article  Google Scholar 

  27. 27.

    Horwitz W, Kamps LR, Boyer RW (1980) Quality assurance in the analysis of foods for trace constituents. J Assoc Off Anal Chem 63:1344–1354

    CAS  Google Scholar 

  28. 28.

    Diaconu R, Vasilov M, Mancas G (2001) Colorimetric assay of salivary nitrite content. J de Med Prev 9:74–78

    Google Scholar 

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Acknowledgements

This work was supported by a Grant of the Romanian National Authority for Scientific Research, CNCS–UEFISCDI, project number PN–RU–PD–2011–3–0081.

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Correspondence to Alin Tudorache.

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Tudorache, A., Ioniţă, D.E., Marin, N.M. et al. In-house validation of a UV spectrometric method for measurement of nitrate concentration in natural groundwater samples. Accred Qual Assur 22, 29–35 (2017). https://doi.org/10.1007/s00769-016-1244-6

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Keywords

  • Nitrate measurement
  • UV spectrometry
  • Groundwater
  • Validation
  • Measurement uncertainty