This paper presents the validation and evaluation of measurement uncertainty of a method for quantitative measurement of nitrate concentration in various groundwater samples by means of molecular UV absorption spectrometry. A calibration study was conducted for the working range from 0.10 mg/L to 6.00 mg/L NO3 −, being accordingly determined detection and quantification limits of 0.07 mg/L NO3 − and 0.10 mg/L NO3 −, respectively. It was also proven that the method is accurate and precise. Selectivity and applicability were discussed. Measurement uncertainty was evaluated based on the validation data.
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Spalding RF, Exner ME (1993) Occurrence of nitrate in groundwater—a review. J Environ Qual 22:392–402
Fan AM, Willhite CC, Book SA (1987) Evaluation of the nitrate drinking water standard with reference to infant methemoglobinemia and potential reproductive toxicity. Regul Toxicol Pharm 7:135–148
Moorcroft MJ, Davis J, Compton RG (2001) Detection and determination of nitrate and nitrite: a review. Talanta 54:785–803
Ito K, Takayama Y, Makabe N, Mitsui R, Hirokawa T (2005) Ion chromatography for measurement of nitrite and nitrate in seawater using monolithic ODS columns. J Chromatogr A 1083:63–67
Zhang J-Z, Fischer CJ (2006) A simplified resorcinol method for direct spectrophotometric measurement of nitrate in seawater. Mar Chem 99:220–226
ISO 7890-3 (1988) Water quality—measurement of nitrate—part 3: spectrometric method using sulfosalicylic acid. International Organization for Standardization (ISO), Geneva
APHA Method 4500-NO3-E (1992) Standard methods for the examination of water and wastewater. American public Health Association, Washington, D.C
APHA Method 4500-NO3-F (1992) Standard methods for the examination of water and wastewater. American public Health Association, Washington, D.C
Brown L, Bellinger EG (1978) Nitrate measurement in fresh and some estuarine waters by ultraviolet light absorption: a new proposed method. Water Res 12:223–229
Drolc A, Vrtovšek J (2010) Nitrate and nitrite nitrogen measurement in waste water using on-line UV spectrometric method. Bioresour Technol 101:4228–4233
Rennie PJ, Sumner AM (1979) Measurement of nitrate in raw, potable and waste waters by ultraviolet spectrophotometry. Analyst 104:837–845
Edwards AC, Hooda PS, Cook Y (2001) Measurement of nitrate in water containing dissolved organic carbon by ultraviolet spectroscopy. Int J Environ Anal Chem 80:49–59
Sah RN (1994) Nitrate–nitrogen determination—a critical review. Commun Soil Sci Plant Anal 25:2841–2869
Greenberg AE, Clesceri LS, Eaton AD (1992) Standard methods for the examination of water and wastewater. American Public Health Association, Washington
Pham HN, Benitez A, Hommet F, Bombe D, Schoefs O, Pauss A (2008) A new quantitative and low-cost determination method of nitrate in vegetables, based on deconvolution of UV spectra. Talanta 76:936–940
Hibbert B (1999) Method validation of modern analytical techniques. Accred Qual Assur 4:352–356
ISO/IEC 17025 (2005) General requirements for the competence of testing and calibration laboratories. International Organization for Standardization (ISO), Geneva
Stefan RI, Baiulescu GE, Aboul-Enein HY, van Staden JF (1999) Estimation of uncertainties in clinical analysis. Accred Qual Assur 4:225–229
Huang H (2014) Uncertainty-based measurement quality control. Accred Qual Assur 19:65–73
Joint Committee for Guides in Metrology (JCGM) (2008) Evaluation of measurement data-guide to the expression of uncertainty in measurement (GUM 1995 with minor corrections) Sèvres, France. http://www.bipm.org/utils/common/documents/jcgm/JCGM_100_2008_E.pdf
ISO 11352 (2012) Water quality-estimation of measurement uncertainty based on validation and quality control data. International Organization for Standardization (ISO), Geneva
GBC Scientific Application Notes-UV visible (1998) Fully automated water nitrate analysis. http://www.gbcscientific.com/appnotes/uv_app_note_007.pdf
ICH Q2 (2005) Validation of analytical procedures: text and methodology. http://www.ich.org/fileadmin/Public_Web_Site/ICH_Products/Guidelines/Quality/Q2_R1/Step4/Q2_R1__Guideline.pdf
Council Directive 98/83/EC (1998) On the quality of water intended for human consumption, The Council of the European Union
Senila M, Drolc A, Pintar A, Senila L, Levei E (2014) Validation and measurement uncertainty evaluation of the ICP-OES method for the multi-elemental measurement of essential and nonessential elements from medicinal plants and their aqueous extracts. J Anal Sci Technol 5:1–9
Tanase IGH, Popa DE, David IG, Buleandra M (2010) Single laboratory validation of a method for the measurement of total inorganic arsenic by hydride generation atomic absorption spectrometry. Anal Lett 43:1172–1189
Horwitz W, Kamps LR, Boyer RW (1980) Quality assurance in the analysis of foods for trace constituents. J Assoc Off Anal Chem 63:1344–1354
Diaconu R, Vasilov M, Mancas G (2001) Colorimetric assay of salivary nitrite content. J de Med Prev 9:74–78
This work was supported by a Grant of the Romanian National Authority for Scientific Research, CNCS–UEFISCDI, project number PN–RU–PD–2011–3–0081.
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Tudorache, A., Ioniţă, D.E., Marin, N.M. et al. In-house validation of a UV spectrometric method for measurement of nitrate concentration in natural groundwater samples. Accred Qual Assur 22, 29–35 (2017). https://doi.org/10.1007/s00769-016-1244-6
- Nitrate measurement
- UV spectrometry
- Measurement uncertainty