Abstract
Two validated, simple and precise densitometric high-performance thin-layer chromatography (HPTLC) quantification methods were proposed for both qualitative and quantitative estimation of oleuropein in Olea europaea leaves and a pharmaceutical product utilizing normal-phase and reversed-phase silica gel TLC plates. In method I, 10 × 20 cm glass plates coated with 0.2 mm thin layers of normal-phase silica gel 60 containing F254 (E-Merck, Germany) and a mixture of ethyl acetate‒methanol‒water (8:1:0.5, V/V) were used as the stationary and the mobile phase, respectively. Method II utilized 10 × 20 cm glass-backed plates supporting 0.2 mm layers of RP-18 silica gel 60 containing F254 (E-Merck, Germany) as the stationary phase and green solvents mixture composed of ethanol‒water (5.5:4.5, V/V) as the mobile phase. The two methods resulted in sharp, symmetrical, well-resolved peaks at RF values of 0.47 ± 0.02 and 0.78 ± 0.03 with linearity ranges 200‒1400 ng/spot (r2 = 0.9994) and 200‒1400 ng/spot (r2 = 0.9996) for method I and method II, respectively. Spots corresponding to oleuropein were scanned at 200 nm. The two methods complied with the ICH guidelines for validation. Due to simplicity, low cost and short analysis time, the methods can be good alternatives for the quality control of different products containing olive leaves extract or pure oleuropein.
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04 May 2021
A Correction to this paper has been published: https://doi.org/10.1007/s00764-021-00100-0
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Acknowledgements
This project was supported by the Research Grant from the Deanship of Scientific Research, Prince Sattam Bin Abdulaziz University, Al-Kharj, Kingdom of Saudi Arabia (Grant No. 2020/03/17308).
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Deanship of Scientific Research, Prince Sattam Bin Abdulaziz University, Al-Kharj, Kingdom of Saudi Arabia (Grant No. 2020/03/17308).
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PA was involved in funding acquisition, methodology, investigation, validation; MTA helped in resources and investigation; HHZ contributed to resources, validation, methodology, conceptualization, writing-reviewing and editing; MHA was involved in validation, methodology, writing-original draft preparation; MSA-K helped in data curation, visualization, supervision, conceptualization, writing-reviewing and editing.
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The original online version of this article was revised: The correct spelling of the co-author's surname is Zaatout.
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Alam, P., Alanazi, M.T., Zattout, H.H. et al. Densitometric high-performance thin-layer chromatography methods for the quantification of oleuropein in Olea europaea leaves and pharmaceutical preparation utilizing normal- and reversed-phase silica gel plates. JPC-J Planar Chromat 33, 609–616 (2020). https://doi.org/10.1007/s00764-020-00066-5
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DOI: https://doi.org/10.1007/s00764-020-00066-5