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Negative effects of a low-quality protein diet on wound healing via modulation of the MMP2 activity in rats

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Protein malnutrition is largely associated with a delay or failure of the healing process. However, the effect of dietary protein quality on wound healing is largely unknown. This study aimed to reveal the effect of dietary protein quality on wound healing and elucidate the regulatory mechanisms in a rat model of full-thickness cutaneous wounds. Rats were fed a normal diet for a week, and then they were divided into three groups that were fed the following diet for the experimental period: casein diet, gluten diet and gluten + lysine diet. The gluten diet significantly decreased body weight and wound healing compared with the casein diet, but this effect was reversed by supplementation with lysine. The numbers of leukocytes were significantly higher in the skin of the gluten group than those in the casein group. The wounded skin tissues of the gluten group showed lower amounts of collagen deposition compared with that in the casein group. Our results also showed that both matrix metalloproteinase (MMP) 2 activity and MMP14 mRNA levels were significantly increased in the skin of the gluten group, compared with the casein group. In summary, this study suggests low-quality protein diets have negative effects on wound healing via modulation of MMP2 activity in rats.

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This work was supported by JSPS KAKENHI (Grant Numbers 26861876).

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Correspondence to Yuichi Oishi.

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The authors report no conflict of interest.

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The experimental protocol was approved by the Animal Care and Research Ethics Committee of the Tokyo University of Agriculture (Permission No. 130611). All animals were treated in accordance with the NIH Guide for the Care and Use of Laboratory Animals.

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Handling Editor: F. Blachier.

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Yamane, T., Konno, R., Iwatsuki, K. et al. Negative effects of a low-quality protein diet on wound healing via modulation of the MMP2 activity in rats. Amino Acids 52, 505–510 (2020).

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