Abstract
This study aimed to assess the effect of rehydration during and after acute aerobic submaximal exercise on total homocysteine (tHcy) concentrations and related parameters in physically active adult males. Twenty trained males (29.4 ± 7.9 years old) completed four exercise tests: two without rehydration during exercise (NH1 and NH2), one with rehydration during exercise using water (H1) and one with rehydration during exercise using an isotonic sports drink (H2). After finishing the exercise tests, subjects followed a rehydration protocol for 2 h. Serum tHcy, vitamin B12, folate, creatine and creatinine were analysed before, after and at 2, 6 and 24 h after exercise. Data were analysed with and without correcting for haemoconcentration to assess the changes in tHcy related. The methylenetetrahydrofolate reductase (MTHFR) 677TT genotype was also analysed. THcy (uncorrected by haemoconcentration) increased significantly after exercise (P < 0.05) in the NH1 and NH2 tests [mean increase ± SD: 1.55 ± 0.33 (15.18%) and 1.76 ± 0.25 (17.69%) µmol/L, respectively], while no significant differences were found in the H1 and H2 tests [mean increase: 0.65 (6.29%) and 0.90 (8.69%) μmol/L, respectively]. The increase was partly due to haemoconcentration and partly due to the metabolism underlying acute exercise. THcy concentrations recovered to baseline after 24 h in all tests. In conclusion, adequate rehydration during acute aerobic exercise using either water or a sports drink maintains tHcy concentrations at baseline and for up to 2 h after exercise in physically active male adults and prevents further increases when compared to no rehydration.
Similar content being viewed by others
References
Alis R et al (2015) Hemoconcentration induced by exercise: revisiting the Dill and Costill equation. Scand J Med Sci Sports 25(6):e630–e637
Arnaoutis G et al (2017) The effect of hypohydration on endothelial function in young healthy adults. Eur J Nutr 56(3):1211–1217. https://doi.org/10.1007/s00394-016-1170-8
Bizheh N, Jaafari M (2011) The effect of a single bout circuit resistance exercise on homocysteine, hs-CRP and fibrinogen in sedentary middle aged men. Iran J Basic Med Sci 14:568
Boreham CA, Kennedy RA, Murphy MH, Tully M, Wallace WF, Young I (2005) Training effects of short bouts of stair climbing on cardiorespiratory fitness, blood lipids, and homocysteine in sedentary young women. Br J Sports Med 39:590–593
Boushey CJ, Berestford SA, Omenn GS, Motulsky AG (1995) A quantitative assessment of plasma homocysteine as a risk factor for cardiovascular disease. JAMA 274:1049–1057
Brosnan JT, Da Silva RP, Brosnan ME (2011) The metabolic burden of creatine synthesis. Amino Acids 40:1325–1331
Casa DJ et al (2000) National athletic trainers’ association position statement: fluid replacement for athletes. J Athl Train 35:212–224
Castañon MM, Lauricella AM, Kordich L, Quintana I (2007) Plasma homocysteine cutoff values for venous thrombosis. Clin Chem Lab Med 45:232–236
Cheuvront SN, Kenefick RW (2014) Dehydration: physiology, assessment, and performance effects. Compr Physiol 4(1):257–285
Choi JK et al (2014) Regular exercise training increases the number of endothelial progenitor cells and decreases homocysteine levels in healthy peripheral blood. Korean J Physiol Pharmacol 18:163–168
Deminice R, Jordao AA (2012) Creatine supplementation reduces oxidative stress biomarkers after acute exercise in rats. Amino Acids 43:709–715. https://doi.org/10.1007/s00726-011-1121-x
Deminice R, Vannucchi H, Simoes-Ambrosio LM, Jordao AA (2011) Creatine supplementation reduces increased homocysteine concentration induced by acute exercise in rats. Eur J Appl Physiol 111:2663–2670. https://doi.org/10.1007/s00421-011-1891-6
Deminice R, Rosa FT, Franco GS, Jordao AA, de Freitas EC (2013) Effects of creatine supplementation on oxidative stress and inflammatory markers after repeated-sprint exercise in humans. Nutrition (Burbank, Los Angel County, Calif) 29:1127–1132. https://doi.org/10.1016/j.nut.2013.03.003
Deminice R, Rosa FT, Franco GS, da Cunha SF, de Freitas EC, Jordao AA (2014) Short-term creatine supplementation does not reduce increased homocysteine concentration induced by acute exercise in humans. Eur J Nutr 53:1355–1361. https://doi.org/10.1007/s00394-013-0636-1
Deminice R, Ribeiro DF, Frajacomo FTT (2016) The effects of acute exercise and exercise training on plasma homocysteine: a meta-analysis. PLoS One 11:e0151653
Dill DB, Costill DL (1974) Calculation of percentage changes in volumes of blood, plasma, and red cells in dehydration. J Appl Physiol 37:247–248
Evans GH, Shirreffs SM, Maughan RJ (2009) Postexercise rehydration in man: the effects of carbohydrate content and osmolality of drinks ingested ad libitum. Appl Physiol Nutr Metab Physiologie appliquee nutrition et metabolisme 34:785–793. https://doi.org/10.1139/H09-065
Frosst P et al (1995) A candidate genetic risk factor for vascular disease: a common mutation in methylenetetrahydrofolate reductase. Nat Genet 10(1):111–113
Fukuda N, Hamajima N, Wakai K, Suzuki K (2014) A cross-sectional study to find out the relationship of methylenetetrahydrofolate reductase (MTHFR) C677T genotype with plasma levels of folate and total homocysteine by daily folate intake in Japanese. J Nutr Sci Vitaminol 60:231–238
Gelecek N, Teoman N, Ozdirenc M, Pinar L, Akan P, Bediz C, Kozan O (2007) Influences of acute and chronic aerobic exercise on the plasma homocysteine level. Ann Nutr Metab 51:53–58
Gibala MJ (2001) Regulation of skeletal muscle amino acid metabolism during exercise. Int J Sport Nutr Exerc Metab 11:87–108
González VC, Perovic NR, Defagó MD (2016) Polimorfismo C677T de la enzima 5, 10-metilenetetrahidrofolato reductasa (MTHFR) y enfermedad cardiovascular. Diaeta 34:40–47
Gonzalez-Alonso J, Crandall CG, Johnson JM (2008) The cardiovascular challenge of exercising in the heat. J Physiol 586:45–53
González-Gross M, Sola R, Castillo MJ (2002) Folato: una vitamina en constante evolución. Medicina clínica 119:627–635
Guzel NA, Pinar L, Colakoglu F, Karacan S, Ozer C (2012) Long-term callisthenic exercise-related changes in blood lipids, homocysteine, nitric oxide levels and body composition in middle-aged healthy sedentary women. Chin J Physiol 55:202–209
Herrmann M, Schorr H, Obeid R, Scharhag J, Urhausen A, Kindermann W, Herrmann W (2003a) Homocysteine increases during endurance exercise. Clin Chem Lab Med CCLM/FESCC 41:1518–1524. https://doi.org/10.1515/CCLM.2003.233
Herrmann M, Schorr H, Obeid R, Scharhag J, Urhausen A, Kindermann W, Herrmann W (2003b) Homocysteine increases during endurance exercise. Clin Chem Lab Med 41:1518–1524
Herrmann M et al (2003c) Comparison of the influence of volume-oriented training and high-intensity interval training on serum homocysteine and its cofactors in young, healthy swimmers. Clin Chem Lab Med CCLM/FESCC 41:1525–1531. https://doi.org/10.1515/CCLM.2003.234
Iglesias-Gutierrez E et al (2012) Transient increase in homocysteine but not hyperhomocysteinemia during acute exercise at different intensities in sedentary individuals. PLoS One 7:e51185. https://doi.org/10.1371/journal.pone.0051185
Jakovljevic B, Gasic B, Kovacevic P, Rajkovaca Z, Kovacevic T (2015) Homocystein as a risk factor for developing complications in chronic renal failure. Mater Socio Med 27:95
Joubert LM, Manore MM (2006) Exercise, nutrition, and homocysteine. Int J Sport Nutr Exerc Metab 16:341–361
Konig D, Bisse E, Deibert P, Muller HM, Wieland H, Berg A (2003) Influence of training volume and acute physical exercise on the homocysteine levels in endurance-trained men: interactions with plasma folate and vitamin B12. Ann Nutr Metab 47:114–118
Kumudini N, Uma A, Naushad SM, Mridula R, Borgohain R, Kutala VK (2014) Association of seven functional polymorphisms of one-carbon metabolic pathway with total plasma homocysteine levels and susceptibility to Parkinson’s disease among South Indians. Neurosci Lett 568:1–5
Laitano O, Kalsi KK, Pearson J, Lotlikar M, Reischak-Oliveira A, Gonzalez-Alonso J (2012) Effects of graded exercise-induced dehydration and rehydration on circulatory markers of oxidative stress across the resting and exercising human leg. Eur J Appl Physiol 112:1937–1944. https://doi.org/10.1007/s00421-011-2170-2
Maroto-Sanchez B, Valtuena J, Albers U, Benito PJ, Gonzalez-Gross M (2013) Acute physical exercise increases homocysteine concentrations in young trained male subjects. Nutr Hosp 28:325–332. https://doi.org/10.3305/nh.2013.28.2.6300
Maroto-Sanchez B, Lopez-Torres O, Palacios G, Gonzalez-Gross M (2016) What do we know about homocysteine and exercise? A review from the literature. Clin Chem Lab Med. https://doi.org/10.1515/cclm-2015-1040
Molina-López J, Molina JM, Chirosa LJ, Florea DI, Sáez L, Planells E (2013) Effect of folic acid supplementation on homocysteine concentration and association with training in handball players. J Int Soc Sports Nutr 10:10
Myers J, Bellin D (2000) Ramp exercise protocols for clinical and cardiopulmonary exercise testing. Sports Med 30:23–29
Okura T et al (2006) Effect of regular exercise on homocysteine concentrations: the HERITAGE Family Study. Eur J Appl Physiol 98:394–401
Petras M, Tatarkova Z, Kovalska M, Mokra D, Dobrota D, Lehotsky J, Drgova A (2014) Hyperhomocysteinemia as a risk factor for the neuronal system disorders. J Physiol Pharmacol 65:15–23
Powers SK, Howley ET (2007) Exercise physiology: theory and application to fitness and performance. McGraw-Hill, New York, NY
Randeva HS et al (2002) Exercise decreases plasma total homocysteine in overweight young women with polycystic ovary syndrome. J Clin Endocrinol Metab 87:4496–4501
Real JT, Merchante A, Gomez JL, Chaves FJ, Ascaso JF, Carmena R (2005) Effects of marathon running on plasma total homocysteine concentrations. Nutr Metab Cardiovasc Dis 15:134–139
Rennie MJ, Tipton KD (2000) Protein and amino acid metabolism during and after exercise and the effects of nutrition. Annu Rev Nutr 20:457–483
Sawka MN, Coyle EF (1999) Influence of body water and blood volume on thermoregulation and exercise performance in the heat. Exerc Sport Sci Rev 27:167–218
Sawka MN, Burke LM, Eichner ER, Maughan RJ, Montain SJ, Stachenfeld NS (2007) American College of Sports Medicine position stand exercise and fluid replacement. Med Sci Sports Exerc 39:377–390
Shen L, Ji H-F (2015) Associations between homocysteine, folic acid, vitamin B12 and Alzheimer’s disease: Insights from meta-analyses. J Alzheimer’s Dis 46:777–790
Shi Z et al (2015) Elevated total homocysteine levels in acute ischemic stroke are associated with long-term mortality. Stroke 46:2419–2425
Sotgia S, Carru C, Caria MA, Tadolini B, Deiana L, Zinellu A (2007) Acute variations in homocysteine levels are related to creatine changes induced by physical activity. Clin Nutr 26:444–449. https://doi.org/10.1016/j.clnu.2007.05.003
Van Hall G, Saltin B, Wagenmakers AJ (1999a) Muscle protein degradation and amino acid metabolism during prolonged knee-extensor exercise in humans. Clin Sci (Lond, Engl 1979) 97:557–567
Van Hall G, Saltin B, Wagenmakers AJM (1999b) Muscle protein degradation and amino acid metabolism during prolonged knee-extensor exercise in humans. Clin Sci 97:557–567
Venta R, Cruz E, Valcarcel G, Terrados N (2009) Plasma vitamins, amino acids, and renal function in postexercise hyperhomocysteinemia. Med Sci Sports Exerc 41:1645–1651. https://doi.org/10.1249/MSS.0b013e31819e02f2
Vincent KR, Braith RW, Bottiglieri T, Vincent HK, Lowenthal DT (2003) Homocysteine and lipoprotein levels following resistance training in older adults. Prev Cardiol 6:197–203
Vincent HK, Bourguignon C, Vincent KR (2006) Resistance training lowers exercise-induced oxidative stress and homocysteine levels in overweight and obese older adults. Obesity 14:1921–1930
Zinellu A, Sotgia S, Zinellu E, Chessa R, Deiana L, Carru C (2006) Assay for the simultaneous determination of guanidinoacetic acid, creatinine and creatine in plasma and urine by capillary electrophoresis UV-detection. J Sep Sci 29:704–708
Acknowledgements
Authors would like to thank Rosa María Torres, Teresa Amigo, Javier Butragueño for laboratory work, Dr. Mercedes Galindo, Dr. Javier Calderón and Dr. Javier Rojo for the medical supervision of the study and Laura Barrios for advice in the statistical analysis of the data.
Funding
The study has been financed with research funds of the ImFINE research group from the Universidad Politécnica de Madrid. Spain.
Author information
Authors and Affiliations
Corresponding authors
Ethics declarations
Conflict of interest
The authors declare no conflicts of interest.
Ethical approval
All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and/or national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards.
Informed consent
Informed consent was obtained from all individual participants included in the study.
Additional information
Handling Editor: H. Jakubowski.
Rights and permissions
About this article
Cite this article
Maroto-Sánchez, B., Lopez-Torres, O., Valtueña, J. et al. Rehydration during exercise prevents the increase of homocysteine concentrations. Amino Acids 51, 193–204 (2019). https://doi.org/10.1007/s00726-018-2655-y
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00726-018-2655-y