Comparative phosphoproteomic analysis reveals differentially phosphorylated proteins regulate anther and pollen development in kenaf cytoplasmic male sterility line

Chen, Peng; Li, Ru; Zhou, Ruiyang

doi:10.1007/s00726-018-2564-0

Original Article
Published: 11 April 2018

Comparative phosphoproteomic analysis reveals differentially phosphorylated proteins regulate anther and pollen development in kenaf cytoplasmic male sterility line

Amino Acids volume 50, pages 841–862 (2018)Cite this article

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Abstract

Cytoplasmic male sterility (CMS) is widely used in plant breeding and represents a perfect model to understand cyto-nuclear interactions and pollen development research. Protein phosphorylation is ubiquitous and is involved in the regulation of diverse cellular processes. To reveal the possible mechanism of CMS and pollen development in kenaf, we performed an iTRAQ-based comparative phosphoproteome analysis in the anthers of a CMS line and wild-type plant (Wt). Whole transcriptome unigenes of kenaf as the reference genome, we identified a total of 3045 phosphorylated sites on 1640 peptides corresponding to 974 unique proteins. 292 of the peptides which corresponding to 247 unique proteins were differentially phosphorylated (fold change ≥ 1.20 with P value< 0.05) between these two materials. 113 and 134 proteins were characterized as up-regulated or down-regulated phosphorylated, respectively. An evaluation of the phosphoproteome and proteomic results indicated that the most significantly phosphorylated proteins were not associated with abundant changes at the protein level. Bioinformatics analysis demonstrated that many of these proteins were involved in various biological processes which may play key roles in pollen development, including carbohydrate metabolism, energy metabolism, transport, gene expression regulation, signal transduction, and cell cycle control. Our results provide insight into the CMS mechanism and pollen development in kenaf from a protein phosphorylation perspective.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (Grant no. 31560341) and the earmarked fund for Modern Agro-industry Technology Research System (CARS-16-E14). We thank LetPub (www.letpub.com) for its linguistic assistance during the preparation of this manuscript.

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Key Laboratory of Plant Genetics and Breeding, College of Agriculture, Guangxi University, Nanning, China
Peng Chen & Ruiyang Zhou
College of Life Science and Technology, Guangxi University, Nanning, China
Ru Li

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Chen, P., Li, R. & Zhou, R. Comparative phosphoproteomic analysis reveals differentially phosphorylated proteins regulate anther and pollen development in kenaf cytoplasmic male sterility line. Amino Acids 50, 841–862 (2018). https://doi.org/10.1007/s00726-018-2564-0

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Received: 18 January 2018
Accepted: 29 March 2018
Published: 11 April 2018
Issue Date: July 2018
DOI: https://doi.org/10.1007/s00726-018-2564-0

Keywords

Kenaf
Pollen
Cytoplasmic male sterility (CMS)
Phosphoproteome
iTRAQ