Selection and identification of novel peptides specifically targeting human cervical cancer
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Cervical cancer is the second most commonly diagnosed cancer and the third leading cause of cancer deaths among females in underdeveloped countries. This study aimed to identify several novel cervical cancer-specific targeting peptides (CSPs) to provide new methods for the effective diagnosis and treatment of cervical cancer. Peptide library screening in vivo was performed on human cervical cancer xenografts with Ph.D.™-12 and C7C phage display peptide libraries. Two specific peptide sequences (GDALFSVPLEVY and KQNLAEG), which were enriched in tumors, were screened, and respectively, named CSP-GD and CSP-KQ through three rounds of biopanning. The in vivo tumor-targeting ability of these peptides was identified by injecting them into mice with cervical cancer xenograft. CSPs were compounded and labeled with fluorescein isothiocyanate (FITC). The specificity and affinity of FITC-CSPs were evaluated in human cervical cancer cell lines and tissue microarrays in vitro by immunofluorescent staining. Results showed that FITC-CSP-GD and FITC-CSP-KQ evidently and specifically bound to the cell membrane and cytoplasm of SiHa, ME-180, and C-33A cells in vitro. In human cervical cancer tissue, FITC-CSP-GD and FITC-CSP-KQ strongly targeted human cervical adenocarcinoma and cervical squamous cell carcinoma tissues, respectively. A bright FITC signal was located mainly on the cell membrane and cytoplasm of tumor cells. In conclusion, the novel 12-residue peptide CSP-GD and 7-residue peptide CSP-KQ could specifically target human cervical cancer and may have the potential to be used in the diagnosis and targeted therapy of cervical cancer.
KeywordsPeptides Tumor targeting Cervical cancer Phage display
We would like to express our gratitude to Dr. Tang and Dr. Liang, who provided valuable guidance in each stage of our paper writing. We would also like to show our appreciation to all individuals who contributed to this paper. This study was supported by the National Natural Science Foundation of China (Grant no. 81472449 and 81101988), the Hunan Provincial Education Department Foundation of China (Grant no. 11C1091), and the Hengyang Industry University Research Project in Hunan Province of China (Grant no. 2015kc53). The study was performed using equipment purchased with funding from the construction program of the key discipline in Hunan Province, China (Basic Medicine Sciences in University of South China).
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Conflict of interest
The authors declare that they have no conflict of interest.
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