Amino Acids

, Volume 49, Issue 7, pp 1177–1192

Comparative proteomic analysis provides insight into 10-hydroxy-2-decenoic acid biosynthesis in honey bee workers

Original Article

Abstract

10-Hydroxy-2-decenoic acid (10-HDA) is the major compound produced from the mandibular glands (MGs) of honey bee workers. However, little information is available on the molecular mechanisms of 10-HDA biosynthesis. In our study, based on investigating the 10-HDA secretion pattern and the morphological characteristics of MGs from honey bee workers of different ages, a comparative proteomic analysis was performed in the MGs of workers with different 10-HDA production. In total, 59 up-regulated protein species representing 45 unique proteins were identified in high 10-HDA-producing workers by 2-DE–MALDI–TOF/TOF MS. These proteins were involved in carbohydrate/energy metabolism, fatty acid metabolism, protein metabolism and folding, antioxidation, cytoskeleton, development and cell signaling. Proteins related to fatty acid metabolism, including fatty acid synthase and β-oxidation enzymes, are potentially crucial proteins involved in 10-HDA biosynthesis pathway. And RNA interference (RNAi) results demonstrated that knockdown of electron transfer flavoprotein subunit beta (ETF-β), one of the protein related to fatty acid metabolism, decreased 10-HDA production of worker bees, suggesting that ETF-β was necessary for 10-HDA biosynthesis. This study reveals the characteristics of MGs of worker bees at different developmental stages and proteins associated with 10-HDA biosynthesis, which provides the first insight into the molecular mechanism of 10-HDA biosynthesis.

Keywords

Honey bee 10-Hydroxy-2-decenoic acid Biosynthesis Proteomics RNAi 

Supplementary material

726_2017_2418_MOESM1_ESM.tif (1.9 mb)
Supplementary material 1 Fig. S1 Chromatogram of 10-HDA measurements by high-performance liquid chromatography (HPLC). a 10-HDA standard, b 10-HDA and the internal standard, c sample from the MGs of worker bees (TIFF 1958 kb)
726_2017_2418_MOESM2_ESM.doc (30 kb)
Supplementary material 2 (DOC 30 kb)
726_2017_2418_MOESM3_ESM.doc (68 kb)
Supplementary material 3 (DOC 68 kb)

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Copyright information

© Springer-Verlag Wien 2017

Authors and Affiliations

  1. 1.Key Laboratory of Shandong Microbial EngineeringQilu University of TechnologyJinanChina
  2. 2.Shandong Academy of Agricultural ScienceJinanChina

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