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Dietary supplementation with l-glutamate and l-aspartate alleviates oxidative stress in weaned piglets challenged with hydrogen peroxide

Amino Acids volume 48, pages 53–64 (2016)Cite this article

Abstract

This study was to evaluate the protective roles of l-glutamate (Glu) and l-aspartate (Asp) in weaned piglets challenged with H2O2. Forty weaned piglets were assigned randomly into one of five groups (8 piglets/group): (1) control group (NC) in which pigs were fed a corn- and soybean meal-based diet and received intraperitoneal administration of saline; (2) H2O2 group (PC) in which pigs were fed the basal diet and received intraperitoneal administration of 10 % H2O2 (1 ml/kg body weight once on days 8 and repeated on day 11); (3) PC + Glu group (PG) in which pigs were fed the basal diet supplemented with 2.0 % Glu before intraperitoneal administration of 10 % H2O2; (4) PC + Asp group (PA) in which pigs were fed the basal diet supplemented with 1.0 % Asp before intraperitoneal administration of 10 % H2O2; (5) PC + Glu + Asp group (PGA) in which pigs were fed the basal diet supplemented with 2.0 % Glu plus 1.0 % Asp before intraperitoneal administration of 10 % H2O2. Measured parameters included daily feed intake (DFI), average daily gain (ADG), feed conversion rate (FCR), and serum anti-oxidative enzyme activities (catalase, superoxide dismutase, glutathione peroxidase-1), serum malondialdehyde and H2O2 concentrations, serum amino acid (AA) profiles, and intestinal expression of AA transporters. Dietary supplementation with Glu, Asp or their combination attenuated the decreases in DFI, ADG and feed efficiency, the increase in oxidative stress, the alterations of serum AA concentrations, and the changed expression of intestinal AA transporters in H2O2-challenged piglets. Thus, dietary supplementation with Glu or Asp alleviates growth suppression and oxidative stress, while restoring serum the amino acid pool in H2O2-challenged piglets.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (Nos. 31110103909, 31272217, 31272450, 31101729, 31201813, 31330075 and 31272451), National Natural Science Foundation of China (no. 31272463), Hunan Provincial Natural Science Foundation of China (NO. 12JJ2014), and Changsha Science and Technology Key program (K1307007-21), The Chinese Academy of Science STS Project (KFJ-EW-STS-063), and Texas A&M AgriLife Research.

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Affiliations

  1. Scientific Observing and Experimental Station of Animal Nutrition and Feed Science in South-Central, Ministry of Agriculture, Hunan Provincial Engineering Research Center of Healthy Livestock, Key Laboratory of Agro-ecological Processes in Subtropical Region, Institute of Subtropical Agriculture, Chinese Academy of Sciences, Changsha, 410125, Hunan, China

    Jielin Duan, Jie Yin, Wenkai Ren, Zhijie Cui, Li Wu, Gang Liu, Xi Wu, Tiejun Li & Yulong Yin

  2. School of Biology, Hunan Normal Univesity, Changsha, 410018, Hunan, China

    Yulong Yin

  3. College of Animal Science and Technology, Hunan Agricultural University, Changsha, 410128, Hunan, China

    Ting Liu & Xingguo Huang

  4. Department of Animal Science, North Carolina State University, Raleigh, 27695, USA

    Sung Woo Kim

  5. Department of Animal Science, Texas A&M University, College Station, TX, 77843, USA

    Guoyao Wu

  6. Changsha Lvye Biotechnology Limited Company Academician Expert Workstation, Changsha, 410128, Hunan, China

    Tiejun Li

  7. Guangdong Wangda Group Academician Workstation for Clean Feed Technology Research and Development in Swine, Guangzhou, 510663, Guangdong, China

    Tiejun Li

  8. Guangdong Hinapharm Group Academician Workstation for Biological Feed and Feed Additives and Animal Intestinal Health, Guangzhou, 511400, Guangdong, China

    Tiejun Li

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  1. Jielin Duan
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Duan, J., Yin, J., Ren, W. et al. Dietary supplementation with l-glutamate and l-aspartate alleviates oxidative stress in weaned piglets challenged with hydrogen peroxide. Amino Acids 48, 53–64 (2016). https://doi.org/10.1007/s00726-015-2065-3

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Keywords

  • l-Glutamate
  • l-Aspartate
  • Oxidative stress
  • Hydrogen peroxide
  • Performance
  • Pigs