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Inflammatory serum proteome pattern in mice fed a high-fat diet

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Abstract

To investigate the influence of diet on serum protein pattern, mice were fed for 8 weeks either control chow or a high-fat diet (containing 21 % w/w milk fat and 0.2 % w/w cholesterol); sera were collected and analyzed by 2-DE. The main positive acute-phase reactant proteins, haptoglobin and hemopexin, were significantly up-regulated in animals receiving the high-fat diet. Data on all other proteins also pointed to an inflammatory condition in these animals. The largest change in concentration was observed for carboxylesterase N, a circulating enzyme seldom connected with lipid metabolism in earlier reports. These observations agree with the notion of a link between diet-induced hyperlipidemia and the inflammatory component of its cardiovascular sequels in humans, but the effects in the experimental animals are massive and obviously affect most of the major serum proteins.

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Abbreviations

apo:

Apolipoprotein

APR:

Acute-phase reactant (protein)

CRP:

C-reactive protein

h :

Human

KO:

Knock-out

m :

Mouse

MUP:

Major urinary protein-1

PPARα:

Peroxisome proliferator-activated receptor α

TG:

Transgenic

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Acknowledgments

This investigation was supported by grants from Fondazione Cariplo, Milano (MC) and from the European Commission SOUTH-LSHM-CT2006-037498 (MC).

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Authors and Affiliations

  1. Gruppo di Studio per la Proteomica e la Struttura delle Proteine, Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, via G. Balzaretti 9, 20133, Milan, Italy

    Elisabetta Gianazza, Cristina Sensi & Ivano Eberini

  2. Laboratorio “Giovanni Galli” di Biochimica e Biologia Molecolare del Metabolismo e Spettrometria di Massa, Dipartimento di Scienze Farmacologiche e Biomolecolari, Università degli Studi di Milano, via G. Balzaretti 9, 20133, Milan, Italy

    Federica Gilardi, Marco Giudici & Maurizio Crestani

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  1. Elisabetta Gianazza
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  2. Cristina Sensi
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  3. Ivano Eberini
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  4. Federica Gilardi
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  5. Marco Giudici
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Correspondence to Elisabetta Gianazza.

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Gianazza, E., Sensi, C., Eberini, I. et al. Inflammatory serum proteome pattern in mice fed a high-fat diet. Amino Acids 44, 1001–1008 (2013). https://doi.org/10.1007/s00726-012-1433-5

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