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Hypoxia-mediated prior induction of monocyte-expressed HSP72 and HSP32 provides protection to the disturbances to redox balance associated with human sub-maximal aerobic exercise

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Abstract

HSP72 is rapidly expressed in response to a variety of stressors in vitro and in vivo (including hypoxia). This project sought a hypoxic stimulus to elicit increases in HSP72 and HSP32 in attempts to confer protection to the sub-maximal aerobic exercise-induced disturbances to redox balance. Eight healthy recreationally active male subjects were exposed to five consecutive days of once-daily hypoxia (2,980 m, 75 min). Seven days prior to the hypoxic acclimation period, subjects performed 60 min of cycling on a cycle ergometer (exercise bout 1—EXB1), and this exercise bout was repeated 1 day post-cessation of the hypoxic period (exercise bout 2—EXB2). Blood samples were taken immediately pre- and post-exercise and 1, 4 and 8 h post-exercise for HSP72 and immediately pre, post and 1 h post-exercise for HSP32, TBARS and glutathione [reduced (GSH), oxidised (GSSG) and total (TGSH)], with additional blood samples obtained immediately pre-day 1 and post-day 5 of the hypoxic acclimation period for the same indices. Monocyte-expressed HSP32 and HSP72 were analysed by flow cytometry, with measures of oxidative stress accessed by commercially available kits. There were significant increases in HSP72 (P < 0.001), HSP32 (P = 0.03), GSSG (t = 9.5, P < 0.001) and TBARS (t = 5.6, P = 0.001) in response to the 5-day hypoxic intervention, whereas no significant changes were observed for GSH (P = 0.22) and TGSH (P = 0.25). Exercise-induced significant increases in HSP72 (P < 0.001) and HSP32 (P = 0.003) post-exercise in EXB1; this response was absent for HSP72 (P ≥ 0.79) and HSP32 (P ≥ 0.99) post-EXB2. The hypoxia-mediated increased bio-available HSP32 and HSP72 and favourable alterations in glutathione redox, prior to exercise commencing in EXB2 compared to EXB1, may acquiesce the disturbances to redox balance encountered during the second physiologically identical exercise bout.

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Abbreviations

EXB1:

Exercise bout 1

EXB2:

Exercise bout 2

GSH:

Reduced glutathione

GSSG:

Oxidised glutathione

HSP:

Heat shock protein

HSP32:

Heme oxygenase

iHSP:

Intracellular heat shock protein

LT:

Lactate threshold

Maximal oxygen consumption:

\( \dot{V}{\text{O}}_{2\max } \)

mHSP72 :

Monocyte heat shock protein

PHER :

Prolonged hypoxic exposure at rest

PO:

Power output

RONS:

Reactive oxygen and/or nitrogen species

TGSH:

Total glutathione

WBGT:

Wet bulb globe temperature

W:

Watts

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Acknowledgments

No external funding was utilised in the completion of this project. The technical and laboratory assistance/support of James Bray and Chris Towlson (laboratory technicians—University of Hull, Department of Sport and Exercise Science) was invaluable in the completion of this project.

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Authors and Affiliations

  1. Muscle Cellular and Molecular Physiology (MCMP) and Applied Sport and Exercise Science (ASEP) Research Groups, Department of Sport and Exercise Sciences, Institute of Sport and Physical Activity Research (ISPAR), University of Bedfordshire, Polhill Campus, Polhill Avenue, Bedford, Bedfordshire, MK41 9EA, UK

    Lee Taylor & Bryna Chrismas

  2. Department of Sport, Health and Exercise Science, University of Hull, Hull, UK

    Lee Taylor, Angela R. Hillman, Adrian W. Midgley, Daniel J. Peart, Bryna Chrismas & Lars R. McNaughton

  3. School of Health Sciences, Bond University, Queensland, Australia

    Lars R. McNaughton

  4. Sport and Physical Activity Department, Edge Hill University, Ormskirk, UK

    Lars R. McNaughton

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  1. Lee Taylor
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  2. Angela R. Hillman
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  3. Adrian W. Midgley
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  4. Daniel J. Peart
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Correspondence to Lee Taylor.

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Taylor, L., Hillman, A.R., Midgley, A.W. et al. Hypoxia-mediated prior induction of monocyte-expressed HSP72 and HSP32 provides protection to the disturbances to redox balance associated with human sub-maximal aerobic exercise. Amino Acids 43, 1933–1944 (2012). https://doi.org/10.1007/s00726-012-1265-3

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