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Proteome analysis of microtubule-associated proteins and their interacting partners from mammalian brain

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Abstract

The microtubule (MT) cytoskeleton is essential for a variety of cellular processes. MTs are finely regulated by distinct classes of MT-associated proteins (MAPs), which themselves bind to and are regulated by a large number of additional proteins. We have carried out proteome analyses of tubulin-rich and tubulin-depleted MAPs and their interacting partners isolated from bovine brain. In total, 573 proteins were identified giving us unprecedented access to brain-specific MT-associated proteins from mammalian brain. Most of the standard MAPs were identified and at least 500 proteins have been reported as being associated with MTs. We identified protein complexes with a large number of subunits such as brain-specific motor/adaptor/cargo complexes for kinesins, dynein, and dynactin, and proteins of an RNA-transporting granule. About 25% of the identified proteins were also found in the synaptic vesicle proteome. Analysis of the MS/MS data revealed many posttranslational modifications, amino acid changes, and alternative splice variants, particularly in tau, a key protein implicated in Alzheimer’s disease. Bioinformatic analysis of known protein–protein interactions of the identified proteins indicated that the number of MAPs and their associated proteins is larger than previously anticipated and that our database will be a useful resource to identify novel binding partners.

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Abbreviations

AMPPCP:

Adenylyl 5′-(β,γ-methylene)diphosphonate

ATP:

Adenosine triphosphate

EDTA:

Ethylenediaminetetraacetic acid

GTP:

Guanosine triphosphate

KLC:

Kinesin light chain

MAPs:

Microtubule-associated proteins

MgcRacGAP:

Rac GTPase-activating protein 1

MKLP-1:

Mitotic kinesin-like protein 1

MTs:

Microtubules

PIPES:

1,4-Piperazinediethanesulfonic acid

PMSF:

Phenylmethanesulfonylfluoride

SVP:

Synaptic vesicle protein

TAME:

N α -p-Tosyl-l-arginine methyl ester

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Authors and Affiliations

  1. The Beatson Institute for Cancer Research, Switchback Road, Bearsden, Glasgow, G61 1BD, Scotland, UK

    Frank Kozielski

  2. The Biotechnology Centre of Oslo, University of Oslo, Gaustadalleen 21, 0349, Oslo, Norway

    Tahira Riaz, Christian J. Koehler, Mario Kroening, Isabel Panse, Margarita Strozynski, Ian M. Donaldson & Bernd Thiede

  3. Institut de Biologie Structurale (CEA-CNRS-UJF), 41 rue Jules Horowitz, 38027, Grenoble Cedex 01, France

    Salvatore DeBonis

  4. The Biotechnology Centre of Oslo, University of Oslo, Gaustadalleen 21, P.O. Box 1125, Blindern, 0317, Oslo, Norway

    Bernd Thiede

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  1. Frank Kozielski
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  2. Tahira Riaz
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  3. Salvatore DeBonis
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  4. Christian J. Koehler
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  5. Mario Kroening
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  6. Isabel Panse
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  7. Margarita Strozynski
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  8. Ian M. Donaldson
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  9. Bernd Thiede
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Correspondence to Bernd Thiede.

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Kozielski, F., Riaz, T., DeBonis, S. et al. Proteome analysis of microtubule-associated proteins and their interacting partners from mammalian brain. Amino Acids 41, 363–385 (2011). https://doi.org/10.1007/s00726-010-0649-5

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  • DOI: https://doi.org/10.1007/s00726-010-0649-5

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