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Transglutaminase-dependent antiproliferative and differentiative properties of nimesulide on B16-F10 mouse melanoma cells

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Abstract

The aim of this study was to collect evidences on the role of transglutaminase (TG, E.C.2.3.2.13) in the antineoplastic properties exerted by nimesulide (NMS), a non-steroidal anti-inflammatory drug, on murine B16-F10 melanoma cells. Treatment of melanoma cells with nimesulide produces a considerable reduction of cell proliferation, paralleled by a remarkable decrease of the intracellular concentration of polyamines spermidine and spermine. NMS treatment induces cancer cell differentiation, likely through the observed enhancement of TG and tyrosinase activities and increase of melanin production, well known markers of melanocyte differentiation. The overall results highlight the possibility that nimesulide acts as antineoplastic agent likely through the induction of intracellular TG activity.

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Acknowledgment

This work was supported by grants from MIUR (PRIN 2004, project no. 2004067819) and from COST Action 922.

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Authors and Affiliations

  1. Department of Biology, University “Tor Vergata”, Via della Ricerca Scientifica, 00133, Rome, Italy

    Angelo Gismondi, Alessandro Lentini, Claudio Tabolacci, Bruno Provenzano & Simone Beninati

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  1. Angelo Gismondi
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  2. Alessandro Lentini
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  3. Claudio Tabolacci
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  4. Bruno Provenzano
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  5. Simone Beninati
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Correspondence to Simone Beninati.

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Gismondi, A., Lentini, A., Tabolacci, C. et al. Transglutaminase-dependent antiproliferative and differentiative properties of nimesulide on B16-F10 mouse melanoma cells. Amino Acids 38, 257–262 (2010). https://doi.org/10.1007/s00726-009-0244-9

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  • DOI: https://doi.org/10.1007/s00726-009-0244-9

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