Summary.
Recently, Guo et al. have reported structural as well as the binding energy data of the particular interactions between the cleavage sites of hemagglutinin and serine proteases, trypsin and furin, using molecular docking approach. Due to a wrong assignment of protonation state on the histidine, one of the catalytic triad in the active site of both enzymes, their docking results are contradictory with the fundamental principle and previous theoretical studies of the known cleavage mechanism in serine proteases.
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References
Fersht AR (1985) Enzyme structure and mechanism, 2nd edn. W. H. Freeman & Co
XL Guo L Li DQ Wei YS Zhu KC Chou (2008) ArticleTitleCleavage mechanism of the H5N1 hemagglutinin by trypsin and furin Amino Acids 35 375–382 Occurrence Handle10.1007/s00726-007-0611-3 Occurrence Handle18235997 Occurrence Handle1:CAS:528:DC%2BD1cXpvVKgtro%3D
L Hedstrom (2002) ArticleTitleSerine protease mechanism and specificity Chem Rev 102 4501–4524 Occurrence Handle12475199 Occurrence Handle10.1021/cr000033x Occurrence Handle1:CAS:528:DC%2BD38XovFCnsrk%3D
S Henrich A Cameron GP Bourenkov R Kiefersauer R Huber I Lindberg W Bode ME Than (2003) ArticleTitleThe crystal structure of the proprotein processing proteinase furin explains its stringent specificity Nature Struct Biol 10 520–526 Occurrence Handle12794637 Occurrence Handle10.1038/nsb941 Occurrence Handle1:CAS:528:DC%2BD3sXkvVentr4%3D
T Ishida S Kato (2003) ArticleTitleTheoretical perspectives on the reaction mechanism of serine proteases: the reaction free energy profiles of the acylation process J Am Chem Soc 125 12035–12048 Occurrence Handle14505425 Occurrence Handle10.1021/ja021369m Occurrence Handle1:CAS:528:DC%2BD3sXmvFehtLw%3D
JJ Perona CS Craik (1995) ArticleTitleStructural basis of substrate specificity in the serine proteases Protein Sci 4 337–360 Occurrence Handle7795518 Occurrence Handle1:CAS:528:DyaK2MXksFyjsL8%3D Occurrence Handle10.1002/pro.5560040301
M Topf PV Várnai WG Richards (2002) ArticleTitleAb initio QM/MM dynamics simulation of the tetrahedral intermediate of serine proteases: insights into the active site hydrogen-bonding network J Am Chem Soc 124 14780–14788 Occurrence Handle12465991 Occurrence Handle10.1021/ja026219q Occurrence Handle1:CAS:528:DC%2BD38Xos1Gqsbg%3D
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Authors’ address: S. Hannongbua, Department of Chemistry, Faculty of Science, Chulalongkorn University, Phayathai Road, Patumwan, Bangkok 10330, Thailand
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Rungrotmongkol, T., Decha, P., Malaisree, M. et al. Comment on “Cleavage mechanism of the H5N1 hemagglutinin by trypsin and furin” [Amino Acids 2008, January 31, Doi: 10.1007/s00726-007-0611-3]. Amino Acids 35, 511–512 (2008). https://doi.org/10.1007/s00726-008-0638-0
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DOI: https://doi.org/10.1007/s00726-008-0638-0